Characterizing multiple sclerosis

ABSTRACT

A method for characterizing multiple sclerosis in a subject involves comparing ratios of expression levels of genes in a biological sample from a subject to references, wherein the multiple sclerosis is characterized based on a difference in the ratios of the expression values of genes in the biological sample from the subject as compared to the references.

RELATED APPLICATIONS

This application claims priority from U.S. Provisional Application Ser. No. 61/533,599 filed Sep. 12, 2011, the entire disclosure of which is incorporated herein by this reference.

GOVERNMENT INTEREST

This invention was made with U.S. government support under contract numbers AI53984 and AI044924 awarded by the National Institutes of Health. The U.S. government has certain rights in the invention.

TECHNICAL FIELD

The presently-disclosed subject matter relates to the characterization of multiple sclerosis (MS) in a subject, including diagnosis of MS and exclusion of a diagnosis of MS.

INTRODUCTION

Detection of brain lesions disseminated in space and time by magnetic resonance imaging (MRI) with gadolinium contrast is a cornerstone in the diagnosis of multiple sclerosis (MS)¹⁻³. Laboratory and clinical findings include detection of immunologic abnormalities in cerebrospinal fluid and evoked potential testing^(4, 5, 31, 32). Clinically isolated syndrome (CIS) is a first neurologic episode lasting at least 24 hours possibly caused by focal inflammation or demyelination^(33, 34). Approximately 10,000-15,000 new diagnoses of MS are made in the United States each year³⁵. Approximately 2-3 times that number experience a CIS each year indicating that a far greater number of subjects experience a CIS than develop MS^(36, 37, 38, 39). The cost to healthcare of determining if a subject with a CIS has MS is significant considering the cost of MRI and additional testing that is performed and the fact that many more subjects have a CIS than develop MS.

Therefore, improved tests that can effectively, efficiently, and noninvasively characterize MS are needed, including tests to diagnose MS and/or to exclude a diagnosis of MS.

SUMMARY

The presently-disclosed subject matter meets some or all of the above-identified needs, as will become evident to those of ordinary skill in the art after a study of information provided in this document.

This Summary describes several embodiments of the presently-disclosed subject matter, and in many cases lists variations and permutations of these embodiments. This Summary is merely exemplary of the numerous and varied embodiments. Mention of one or more representative features of a given embodiment is likewise exemplary. Such an embodiment can typically exist with or without the feature(s) mentioned; likewise, those features can be applied to other embodiments of the presently-disclosed subject matter, whether listed in this Summary or not. To avoid excessive repetition, this Summary does not list or suggest all possible combinations of such features.

The presently-disclosed subject matter includes methods useful for characterizing an auto-immune disease, and more particularly, for characterizing multiple sclerosis. The presently-disclosed subject matter further includes kits and devices useful for characterizing an auto-immune disease.

In some embodiments, a method for characterizing multiple sclerosis (MS) in a subject involves providing a biological sample from the subject; determining expression levels of at least two genes in the biological sample; calculating one or more ratios of the expression levels of the at least two genes; and comparing each ratios to a reference, wherein the is multiple sclerosisis characterized based on a difference in the ratios of the expression values of the at least two genes in the biological sample from the subject as compared to the references.

The at least two genes can be selected from those represented by SEQ ID NOs: 1-47, those corresponding to the genes set forth in Table A, or those corresponding to the genes set forth in Table B. In embodiments of the method, the expression levels of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes can be determined. In some embodiments, expression levels of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP are determined. In some embodiments, expression levels of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP are determined.

In accordance with the presently-disclosed subject matter, ratios of expression levels of genes are used to characterize an auto-immune disease. In this regard, ratios of interest for use in characterizing MS in a subject include the one or more ratios of expression levels of genes corresponding to those set forth in Table A, wherein each ratio is calculated by dividing the expression level of a first gene in Table A by the expression level of a second gene in Table A. In some embodiments, the at least one ratio is selected from the ratios set forth in Table B. In some embodiments, the one or more ratios consist of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, or 83 ratios set forth in Table B. In some embodiments, the one or more ratios consist of the ratios set forth in Column 1 (MS vs. CTRL) of Table B. In some embodiments, the one or more ratios consist of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, or 42 ratios set forth in Column 1 (MS vs. CTRL) of Table B. In some embodiments, the one or more ratios consist of the ratios set forth in Column 2 (MS vs. OND) of Table B. In some embodiments, the one or more ratios consist of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, or 41 ratios set forth in Column 2 (MS vs. OND) of Table B.

Various references can be selected for use in accordance with the presently-disclosed subject matter. In some embodiments, the reference is a standard reference ratio or a threshold value. For another example, in some embodiments, the reference is a reference ratio of a comparator group. In some embodiments, a “comparator group” or “reference group” includes individuals having a common characterization, for example, healthy control individuals, individuals who have been diagnosed with a condition often confused with an auto-immune disease of interest in the context of clinical diagnosis, individuals who have been diagnosed with an auto-immune disease of interest, or individuals who have another common characterization of interest. Expression values of biomarkers obtained from biological samples of individuals in a comparator group can be used to calculate reference ratios.

Methods of the presently-disclosed subject matter and also include comparing each subject ratio to a second reference. For example, in some embodiments, the reference can be a healthy control, and the second reference is not a healthy control. In some embodiments, the second reference comprises other neurologic disorders (OND).

Characterizing MS in a subject is inclusive of providing a diagnosis, prognosis and/or theranosis of the condition. As such, in some embodiments, characterization comprises diagnosing or prognosticating MS. In some embodiments, MS is predicted. In some embodiments, MS is not predicted. In some embodiments, the characterization comprises an exclusion of a diagnosis of MS. In some embodiments, the method also includes providing a series of biological sample obtained from the subject over a period of time. A change in the ratios in each of the biological samples from the subject can be useful for characterizing MS in the subject.

The presently-disclosed subject matter further includes kits and devices useful for detecting and/or determining expression levels of at least two genes in a biological sample.

The kits of the presently-disclosed subject matter can include primer pairs for determining expression levels of at least two genes, which can be useful for calculating ratios as disclosed herein. In some embodiments, the kit includes primer pairs for determining expression levels of at least two genes represented by SEQ ID NOs: 1-47. In some embodiments, the kit includes primer pairs for determining expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes represented by SEQ ID NOs: 1-47. In some embodiments, the kit includes primer pairs for determining expression levels of at least two genes corresponding to those set forth in Table A. In some embodiments, the kit includes primer pairs for determining expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes corresponding to those set forth in Table A. In some embodiments, the kit includes primer pairs for determining expression levels of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP. In some embodiments, the kit includes primer pairs for determining expression levels of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. In some embodiments, the kit includes primer pairs for determining expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, or 34 genes corresponding to those set forth in Table B.

The devices of the presently-disclosed subject matter can include a probe for selectively binding each of at least two gene expression products to detect at least two genes, which can be useful for determining expression levels of the genes and for calculating ratios as disclosed herein. Such probes can selectively bind the gene products, for example, by hybridization of the probe and a nucleotide gene product. In some embodiments, the device includes probes for detecting each of at least two genes represented by SEQ ID NOs: 1-47. In some embodiments, the device includes probes for detecting each of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes represented by SEQ ID NOs: 1-47. In some embodiments, the device includes probes for detecting each of at least two genes corresponding to those set forth in Table A. In some embodiments, the device includes probes for detecting each of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes corresponding to those set forth in Table A. In some embodiments, the device includes probes for detecting each of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP. In some embodiments, the device includes probes for detecting each of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. In some embodiments, the device includes probes for detecting each of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, or 34 genes corresponding to those set forth in Table B.

BRIEF DESCRIPTION OF THE DRAWINGS

The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are used, and the accompanying drawings of which:

FIG. 1 Gene expression profiles across multiple autoimmune diseases. Expression levels of 44 target genes were determined by quantitative RT-PCR and normalized to expression of GAPDH. Expression levels of 31 genes are shown; expression levels of the remainder were not statistically different between CTRL and any disease cohort. Genes are identified that showed statistically significant [P <0.05 after Bonferroni's correction] increased or decreased expression in individual disease cohorts relative to CTRL subjects. Numerical expression ratios [disease group average/CTRL average] are displayed within the colored boxes.

FIG. 2 Discrimination between MS and CTRL subjects with an 8 ratio scoring system. (a) Performance of the single ratio, ANAPC1/CHEK2 to discriminate MS and CTRL subjects. (b) Genes making up 8 unique discriminatory ratios. P values compare expression levels of ratios between MS and CTRL subjects. (c) Increased sensitivity with increasing numbers of ratios. (d) Score distributions among subjects using 8 ratios. (e) Validation of results by analyzing 40 new MS subjects and 40 new CTRL subjects. (f) Score distribution between OND-I and OND-NI subjects. (g) Mean scores±std. dev. among subjects with CIS, initial diagnosis of MS, and established MS. P value is not significant among groups. (h) Mean scores±std. dev. among MS subjects from different geographic locations. P value is not significant among groups.

FIG. 3 Discrimination of MS subjects from subjects with inflammatory neurologic diseases, TM or NMO. Most discriminatory gene expression ratios were identified that segregate MS subjects from TM and NMO subjects (CTRL is included for reference). The point system was applied to combine ratio performance into a single discriminator.

FIG. 4 Discrimination of subjects with Parkinson's disease from MS and CTRL. Most discriminatory gene expression ratios were identified that segregate Parkinson's disease subjects from MS subjects and CTRL subjects. Using the point system, the % of Parkinson's subjects with a score >0, Y-axis, relative to the number of ratios, X-axis, for the different comparator groups was determined.

FIG. 5 Discrimination of MS subjects from heterogeneous comparator groups. The top 15 gene expression ratios with the greatest ability to discriminate MS from OND-I, OND-NI, or ALL (OND-I, OND-NI, and CTRL) were identified. Using the point system, the % of MS subjects with a score >0, Y-axis, relative to the number of ratios, X-axis, for the different comparator groups [CTRL is included for reference] were determined.

FIG. 6 Discrimination between MS and OND-I subjects using 10 gene expression ratios. (a) Genes making up 10 unique discriminatory ratios. P values compare individual ratio values between MS and OND-I subjects. (b) Increasing number of ratios increases sensitivity or ability to discriminate between MS and OND-I subjects. (c) The score distribution in MS and OND-I subjects using 10 ratios. (d) Validation of results by analyzing 40 new MS subjects and 40 new OND-I subjects (20 TM+20 NMO). (e) Mean scores±std. dev. among subjects with CIS, initial diagnosis of MS and established MS. P is not significant for CIS versus MS naïve, 0.03 for CIS versus established MS, and <0.0001 for MS naïve versus established MS. (f) Mean scores±std. dev. among subjects based upon geographic sites. P is not significant for Nashville versus Europe, <0.0001 for Nashville versus U.S. non-Nashville, and <0.0001 for Europe versus U.S. non-Nashville. (g) Score distributions between [CIS and MS-naïve] and established MS.

FIG. 7 Discrimination between MS and OND-NI subjects using 10 gene expression ratios. (a) Identification of genes making up the 10 unique discriminatory ratios. P values compare individual ratio values between MS and OND-NI subjects. (b) Increasing the number of gene expression ratio increases the ability to discriminate between MS and OND-NI subjects. (c) Score distribution using 10 ratios in the training set. (d) Validation of results by analyzing 40 new MS subjects and 40 new OND-NI subjects. (e) Mean scores±std. dev. among subjects with CIS, initial diagnosis of MS and established MS. P values were not significant among any of the comparisons. (f) Mean scores±std. dev. among subjects based upon geographic sites. P values were not significant for any of the comparisons.

FIG. 8 Flow chart describing sample collection and processing, data generation, and methods of data analysis.

FIG. 9 Gene-expression profiles in subjects with CIS, MS-naïve or MS-established. (a) Expression levels of 23 target genes were determined by quantitative reverse-transcription PCR and normalized to expression of GAPDH. Results are expressed as the ratio of the expression level of the indicated genes in the disease cohort relative to the CTRL cohort, log₂. Genes are identified that showed statistically significant (P <0.05 after Bonferroni's correction for multiple testing) increased or decreased expression. Numerical expression ratios, log), of the test/CTRL cohorts are displayed within the boxes. (b) Cumulative percentage of over- and under-expressed genes in each disease cohort relative to CTRL. (c) Statistical significance of the expression level of each target gene between each disease cohort and CTRL was determined using Student's T test. P values are expressed as log₁₀.

FIG. 10 (a) Ratios that make up the ratioscore discriminating MS from CTRL. Columns represent individual ratios. Rows represent individual subjects within the MS cohort. Black/dark grey in the heatmap denotes individual subjects with the value of the individual ratio greater than the value of the ratio in all subjects within the CTRL cohort. Light grey/white denotes individual subjects with the value of the individual ratio less than or equal to the highest ratio value in all subjects within the CTRL cohort. (b) Results from inputting independent CIS→MS subjects into the ratioscore algorithm.

FIG. 11 (a) The ratioscore method discriminates between MS and OND subjects. Ratios that make up the ratioscore to discriminate MS from OND. Columns represent individual ratios. Rows represent individual subjects within the MS cohort. Black/dark grey in the heatmap denotes individual subjects with the value of the individual ratio greater than the value of the ratio in all subjects within the CTRL cohort. Light grey/white denotes individual subjects with the value of the individual ratio less than or equal to the highest ratio value in all subjects within the CTRL cohort. (b) Results from inputting independent CIS→MS subjects into the ratioscore algorithm.

FIG. 12 a. Ability of the radioscore method to discriminate between MS and combined CTRL plus OND subjects. Columns represent individual ratios. Rows represent individual subjects within the MS cohort. Black/dark grey in the heatmap denotes individual subject with the value of the individual ratio greater than the value of the ratio in all subjects within the CTRL cohort. Light grey/white denotes individual subjects with the value of the individual ratio less than or equal to the highest ratio value in all subjects within the CTRL cohort. b. Results from inputting independent CIS→MS subjects into the ratioscore alcorithm.

FIG. 13 Ratios making up the ratioscore that discriminate MS from OND-NI or OND-I. a. Optimum ratios to discriminate MS from OND-I. b. Results for individual CIS→MS subjects using the MS: OND-I ratioscore. c. Optimum ratios to discriminate MS from OND-NI. d. Results for individual CIS→MS subjects using the OND-NI ratioscore.

DESCRIPTION OF EXEMPLARY EMBODIMENTS

The details of one or more embodiments of the presently-disclosed subject matter are set forth in this document. Modifications to embodiments described in this document, and other embodiments, will be evident to those of ordinary skill in the art after a study of the information provided in this document. The information provided in this document, and particularly the specific details of the described exemplary embodiments, is provided primarily for clearness of understanding and no unnecessary limitations are to be understood therefrom. In case of conflict, the specification of this document, including definitions, will control.

The presently-disclosed subject matter includes methods, devices, and kits useful for characterizing an auto-immune disease in a subject and, more particularly, for characterizing multiple sclerosis (MS) in a subject. In some embodiments, the method involves providing a biological sample from the subject; determining expression values of at least two genes in the biological sample; calculating one or more ratios of the expression values of the at least two genes; and comparing each ratios to a reference, wherein the MS is characterized based on a difference in the ratios of the expression values of the at least two genes in the biological sample from the subject as compared to the references. In some embodiments, the biological sample is blood obtained from the subject or another biological sample containing a cell obtained from the subject, e.g., a subject suspected of having MS. The method can be used, in some embodiments, to diagnose the subject with MS. In some embodiments, the method can be used to exclude the subject from a diagnosis of MS.

Methods of the presently-disclosed methods include determining expression values of genes in biological samples. As such, nucleic acid molecules or nucleotides are relevant to the disclosed subject matter. Nucleotides or genes, the expression of which is desired to be determined for characterizing an auto-immune disease, include, but are not limited to those identified in Table A, the isolated nucleic acid molecules of any one of SEQ ID NOs: 1-47, fragments of the isolated nucleic acid molecules of any one of SEQ ID NOs: 1-47 where detection of such fragments are indicative of expression of an associated gene, e.g., as identified in Table A, complementary nucleic acid molecules, isolated nucleic acid molecules capable of hybridizing to any one of the SEQ ID NOs: 1-47 under conditions disclosed herein, and corresponding RNA and/or DNA molecules.

As used herein, “nucleic acid” and “nucleic acid molecule” refer to any of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), oligonucleotides, fragments generated by the polymerase chain reaction (PCR), and fragments generated by any of ligation, scission, endonuclease action, and exonuclease action. The term “isolated”, when used in the context of an isolated DNA molecule or an isolated polypeptide, is a DNA molecule or polypeptide that, by the hand of man, exists apart from its native environment and is therefore not a product of nature.

Unless otherwise indicated, a particular nucleotide sequence also implicitly encompasses complementary sequences, subsequences, elongated sequences, as well as the sequence explicitly indicated. The terms “nucleic acid molecule” or “nucleotide sequence” can also be used in place of “gene”, “cDNA”, or “mRNA”. Nucleic acids can be derived from any source, including any organism. In one embodiment, a nucleic acid is derived from a biological sample isolated from a subject.

The terms “complementary” and “complementary sequences”, as used herein, refer to two nucleotide sequences that comprise antiparallel nucleotide sequences capable of pairing with one another upon formation of hydrogen bonds between base pairs. As used herein, the term “complementary sequences” means nucleotide sequences which are substantially complementary, as can be assessed by the same nucleotide comparison set forth herein, or is defined as being capable of hybridizing to the nucleic acid segment in question under conditions such as those described herein. In one embodiment, a complementary sequence is at least 80% complementary to the nucleotide sequence with which is it capable of pairing. In another embodiment, a complementary sequence is at least 85% complementary to the nucleotide sequence with which is it capable of pairing. In another embodiment, a complementary sequence is at least 90% complementary to the nucleotide sequence with which is it capable of pairing. In another embodiment, a complementary sequence is at least 95% complementary to the nucleotide sequence with which is it capable of pairing. In another embodiment, a complementary sequence is at least 98% complementary to the nucleotide sequence with which is it capable of pairing. In another embodiment, a complementary sequence is at least 99% complementary to the nucleotide sequence with which is it capable of pairing. In still another embodiment, a complementary sequence is at 100% complementary to the nucleotide sequence with which is it capable of pairing. A particular example of a complementary nucleic acid segment is an antisense oligonucleotide.

“Stringent hybridization conditions” in the context of nucleic acid hybridization experiments are both sequence- and environment-dependent. Longer sequences hybridize specifically at higher temperatures. Generally, highly stringent hybridization and wash conditions are selected to be about 5° C. lower than the thermal melting point (T_(m)) for the specific sequence at a defined ionic strength and pH. The T_(m) is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched probe. Very stringent conditions are selected to be equal to the T_(m) for a particular probe. Typically, under “stringent conditions” a probe hybridizes specifically to its target sequence, but to no other sequences. An extensive guide to the hybridization of nucleic acids is found in Tijssen 1993, which is incorporated herein by this reference. In general, a signal to noise ratio of 2-fold (or higher) than that observed for a negative control probe in a same hybridization assay indicates detection of specific or substantial hybridization.

It is understood that in order to determine a gene expression level by hybridization, a full-length cDNA need not be employed. To determine the expression level of a gene represented by one of SEQ ID NOs: 1-47, any representative fragment or subsequence of the sequences set forth in SEQ ID NOs: 1-47 can be employed in conjunction with the hybridization conditions disclosed herein. As a result, a nucleic acid sequence used to assay a gene expression level can comprise sequences corresponding to the open reading frame (or a portion thereof), the 5′ untranslated region, and/or the 3′ untranslated region. It is understood that any nucleic acid sequence that allows the expression level of a reference gene to be specifically determined can be employed with the methods and compositions of the presently disclosed subject matter.

As used herein, the terms “corresponding to” and “representing”, “represented by” and grammatical derivatives thereof, when used in the context of a nucleic acid sequence corresponding to or representing a gene, refers to a nucleic acid sequence that results from transcription, reverse transcription, or replication from a particular genetic locus, gene, or gene product (for example, an mRNA). In other words, a partial cDNA, or full-length cDNA corresponding to a particular reference gene is a nucleic acid sequence that one of ordinary skill in the art would recognize as being a product of either transcription or replication of that reference gene (for example, a product produced by transcription of the reference gene). One of ordinary skill in the art would understand that the partial cDNA, or full-length cDNA itself is produced by in vitro manipulation to convert the mRNA into a cDNA, for example by reverse transcription of an isolated RNA molecule that was transcribed from the reference gene. One of ordinary skill in the art will also understand that the product of a reverse transcription is a double-stranded DNA molecule, and that a given strand of that double-stranded molecule can embody either the coding strand or the non-coding strand of the gene. The sequences presented in the Sequence Listing are single-stranded, however, and it is to be understood that the presently claimed subject matter is intended to encompass the genes represented by the sequences presented in SEQ ID NOs: 1-47, including the specific sequences set forth as well as the reverse/complement of each of these sequences.

The term “gene expression” generally refers to the cellular processes by which a biologically active polypeptide is produced from a DNA sequence. Generally, gene expression comprises the processes of transcription and translation, along with those modifications that normally occur in the cell to modify the newly translated protein to an active form and to direct it to its proper subcellular or extracellular location.

The terms “gene expression level” and “expression level” as used herein refer to an amount of gene-specific RNA or polypeptide that is present in a biological sample. When used in relation to an RNA molecule, the term “abundance” can be used interchangeably with the terms “gene expression level” and “expression level”.

Determination of expression levels of genes of interest can be achieved using any technique known the skilled artisan. For example, in some embodiments, RNA can be purified from the biological sample, converted to the more-stable complementary DNA (cDNA), before the gene expression products of genes of interest are detected. As will be recognized by the skilled artisan, where amplification of the sample is desired, polymerase chain reaction amplification can be employed. Determining the expression levels can be achieved, for example, using reverse transcription-polymerase chain reaction (RT-PCR), microarray analysis, or other techniques known to the skilled artisan.

In some embodiments, determining the expression levels of genes in the biological sample includes determining the expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, or 47 genes represented by SEQ ID NOs: 1-47. In some embodiments, determining the expression levels of genes in the biological sample includes determining the expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes corresponding to those set forth in Table A.

TABLE A Genes Gene ABI Assay SEQ ID Abbreviation Gene NCBI Ref. No. Number: NO: ABR active BCR-related gene, transcript NM_001159746.1 Hs00254300_m1 1 variant 3 ACTB actin, beta NM_001101.3 Hs99999903_m1 2 ACTR1A ARP1 actin-related protein 1 NM_005736.3 Hs00194913_m1 3 homolog A, centractin alpha (yeast) ADAMTSL4 ADAMTS-like 4 (ADAMTSL4), NM_019032.4 Hs00296775_m1 4 transcript variant 1 ANAPC1 anaphase promoting complex NM_022662.2 Hs00224096_m1 5 subunit 1 APOBEC3F apolipoprotein B mRNA editing NM_145298.5 Hs00272529_m1 6 enzyme, catalytic polypeptide-like 3F ASL argininosuccinate lyase NM_001024943.1 Hs00163695_m1 7 B2M beta-2-microglobulin NM_004048.2 Hs99999907_m1 8 BRCA1 breast cancer 1, early onset NR_027676.1 Hs00173237_m1 9 (BRCA1), transcript variant 6, non- coding RNA CD55 CD55 molecule, decay accelerating NM_000574.3 Hs00167090_m1 10 factor for complement (Cromer blood group), transcript variant 1 CDH1 cadherin 1, type 1, E-cadherin NM_004360.3 Hs00170423_m1 11 (epithelial) CDKN1B cyclin-dependent kinase inhibitor NM_004064.3 Hs00153277_m1 12 1B (p27, Kip1) CHEK2 checkpoint kinase 2 (CHEK2), NM_001005735.1 Hs00200485_m1 13 transcript variant 3 CSF3R colony stimulating factor 3 receptor NM_156039.3 Hs00167918_m1 14 (granulocyte), transcript variant 3 CTSS cathepsin S, transcript variant 1 NM_004079.4 Hs00175403_m1 15 EPHX2 epoxide hydrolase 2, cytoplasmic NM_001979.4 Hs00157403_m1 16 EXT2 exostosin 2, transcript variant 2 NM_207122.1 Hs00181158_m1 17 FOS FBJ murine osteosarcoma viral NM_005252.3 Hs00170630_m1 18 oncogene homolog FOSL1 FOS-like antigen 1 NM_005438.3 Hs00759776_s1 19 FOXN3 forkhead box N3, transcript variant 1 NM_001085471.1 Hs00231993_m1 20 GAPDH-1 glyceraldehyde-3-phosphate NM_002046.3 Hs99999905_m1 21 dehydrogenase GAPDH-2 glyceraldehyde-3-phosphate NM_002046.3 Hs99999905_m1 22 dehydrogenase GATA3 GATA binding protein 3 NM_001002295.1 Hs00231122_m1 23 GNB5 guanine nucleotide binding protein NM_006578.3 Hs00275095_m1 24 (G protein), beta 5, transcript and variant 1 Hs01034253_m1 GSTM4 glutathione S-transferase mu 4, NM_147148.2 Hs00426432_m1 25 transcript variant 2 HLA-DRA major histocompatibility complex, NM_019111.4 Hs00219575_m1 26 class II, DR alpha HRAS v-Ha-ras Harvey rat sarcoma viral NM_001130442.1 Hs00610483_m1 27 oncogene homolog (HRAS), transcript variant 3 IFI27 interferon, alpha-inducible protein NM_001130080.1 Hs00271467_m1 28 27 (IFI27), transcript variant 1 IL11RA interleukin 11 receptor, alpha, NM_001142784.1 Hs00234415_m1 29 transcript variant 3 JUN jun proto-oncogene NM_002228.3 Hs00277190_s1 30 KRAS v-Ki-ras2 Kirsten rat sarcoma viral NM_004985.3 Hs00270666_m1 31 oncogene homolog, transcript variant b LEPREL4 leprecan-like 4 NM_006455.2 Hs00197668_m1 32 LLGL2 lethal giant larvae homolog 2 NM_001015002.1 Hs00189729_m1 33 (Drosophila), transcript variant 2 NRAS neuroblastoma RAS viral (v-ras) NM_002524.4 Hs00180035_m1 34 oncogene homolog OAS1 2′-5′-oligoadenylate synthetase 1, NM_001032409.1 Hs00242943_m1 35 40/46 kDa, transcript variant 3, ORC1 origin recognition complex, subunit NM_001190819.1 Hs00172751_m1 36 1 (ORC1), transcript variant 3 PGK1 phosphoglycerate kinase 1 NM_000291.3 Hs99999906_m1 37 PMAIP1 phorbol-12-myristate-13-acetate- NM_021127.2 Hs00560402_m1 38 induced protein 1 POU6F1 POU class 6 homeobox 1, NR_026893.1 Hs00231276_m1 39 transcript variant 2 RANGAP1 Ran GTPase activating protein 1 NM_002883.2 Hs00610049_m1 40 SPIB Spi-B transcription factor (Spi-1/PU.1 NM_003121.3 Hs00162150_m1 41 related) TAF11 TAF11 RNA polymerase II, TATA NM_005643.2 Hs00194573_m1 42 box binding protein (TBP)- associated factor, 28 kDa TBP TATA box binding protein, NM_001172085.1 Hs00427620_m1 43 transcript variant 2 TGFBR2 transforming growth factor, beta NM_001024847.2 Hs00559661_m1 44 receptor II (70/80 kDa), transcript variant 1 TP53 tumor protein p53 (TP53), NM_001126113.1 Hs00153340_m1 45 transcript variant 4 TP53-2 tumor protein p53 (TP53), NM_001126112.1 Hs01034253_m1 46 transcript variant 2 TXK TXK tyrosine kinase NM_003328.2 Hs00177433_m1 47 IL11R1

In some embodiments, determining the expression levels of genes in the biological sample includes determining the expression levels of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP. In some embodiments, determining the expression levels of genes in the biological sample includes determining the expression levels of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. In some embodiments, determining the expression levels of genes in the biological sample includes determining the expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, or 34 genes corresponding to those set forth in Table B.

As used herein, a “ratio” or “expression ratio” is the expression value of a first biomarker (numerator) divided by the expression value of a second biomarker (denominator), e.g., Gene A/Gene B. As such, once the expression levels of at least two genes are determined, a ratio can be calculated. Ratios can be calculated using expression levels of genes in a biological sample obtained from a subject. In some embodiments, a reference can be a ratio calculated using expression levels of genes from another source. As such, the term “subject ratio” can used herein to refer to a ratio calculated using expression values of a gene pair in a biological sample obtained from a subject, while the term “reference ratio” can be used to refer to a ratio of the same biomarker pair in a reference sample, which serves as a reference to which the subject ratio is compared.

TABLE B Ratios MS vs. CTRL MS vs. OND Expression Ratios Expression Ratios Numerator Denominator Numerator Denominator JUN CD55 PGK1 CTSS JUN SPIB CDKN1B GAPDH-1 TAF11 FOS KRAS CTSS PMAIP1 TBP ACTB TBP TAF11 FOSL1 APOBEC3F GAPDH-2 KRAS ASL KRAS OAS1 GATA3 ANAPC1 KRAS ASL B2M FOSL1 CSF3R CD55 OAS1 GAPDH-2 OAS1 GSTM4 TBP GSTM4 CSF3R TBP CTSS CDKN1B APOBEC3F LLGL2 PMAIP1 ASL APOBEC3F TAF11 GSTM4 CDKN1B TP53-1 CDKN1B TP53 LLGL2 FOS CD55 GATA3 LLGL2 APOBEC3F CDKN1B GAPDH-2 EXT2 OAS1 B2M GAPDH-1 EXT2 GNB5 EXT2 RANGAP1 ASL RANGAP1 IL11RA TP53 POU6F1 FOS B2M CSF3R LLGL2 TGFBR2 B2M IL11RA EXT2 APOBEC3F ASL IL11R1 TAF11 TGFBR2 CDKN1B ANAPC1 LLGL2 ANAPC1 EXT2 FOS OAS1 APOBEC3F TBP ANAPC1 POU6F1 GSTM4 EXT2 CSF3R CDKN1B GNB5 CDKN1B GSTM4 EXT2 TGFBR2 EXT2 ANAPC1 ASL JUN PMAIP1 HLA-DRA GNB5 RANGAP1 TBP TP53-2 KRAS ANAPC1 ASL GSTM4 EPHX2 TP53-2 B2M GAPDH-1 TBP TBP CTSS EPHX2 OAS1 TP53-2 CTSS JUN TAF11 EPHX2 PMAIP1 RANGAP1 EPHX2 ACTB ASL CSF3R TBP ASL PMAIP1 HLA-DRA LLGL2 OAS1 FOSL1 ANAPC1 TBP CSF3R TP53-1 ANAPC1 EPHX2 EPHX2 CDKN1B CTSS CD55 CTSS EXT2 TP53-2 ACTB B2M CD55 CTSS PMAIP1

In embodiments of the presently-disclosed subject matter, the method involves calculating one or more ratios of expression levels of genes corresponding to those set forth in Table A, wherein each ratio is calculated by dividing the expression level of a first gene in Table A by the expression level of a second gene in Table A.

In embodiments of the presently-disclosed subject matter, the method involves calculating one or more ratios set forth in Table B. In some embodiments, the method includes calculating 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, or 83 ratios set forth in Table B.

In embodiments of the presently-disclosed subject matter, the method involves calculating one or more ratios set forth in Column 1 (MS vs. CTRL) of Table B. In some embodiments, the method includes calculating 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, or 42 ratios set forth in Column 1 (MS vs. CTRL) of Table B.

In embodiments of the presently-disclosed subject matter, the method involves calculating one or more ratios set forth in Column 2 (MS vs. OND) of Table B. In some embodiments, the method includes calculating 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, or 41 ratios set forth in Column 2 (MS vs. OND) of Table B.

Various references are appropriate for use in connection with the presently-disclosed subject matter, with non-limiting examples described herein. In some embodiments, the reference comprises a reference ratio calculated using of the expression level of two genes in a biological sample taken from one or more individuals, which two genes are the same two genes used to calculate the subject ratio. The expression levels of genes in biological samples from one or more individuals can be a expression levels from a reference group or comparator group.

In some embodiments, a “comparator group” or “reference group” includes individuals having a common characterization, for example, healthy control individuals, individuals who have been diagnosed with a condition often confused with an auto-immune disease of interest in the context of clinical diagnosis, individuals who have been diagnosed with an auto-immune disease of interest, or individuals who have another common characterization of interest. Expression values of biomarkers obtained from biological samples of individuals in a comparator group can be used to calculate reference ratios. Data associated with one or more comparator groups can be stored, for example, in a database that can be accessed when practicing a method in accordance with the presently-disclosed subject matter.

With reference to Table B, for example, ratios-of-interest are provided for use with a healthy control comparator group (CTRL, column 1) or a comparator group of individuals having other neurologic disorders (OND, column 2). Examples of comparator groups relevant to characterization of MS include, but are not limited to: healthy control (CTRL), clinically isolated syndrome (CIS), CIS later developing MS (CIS→MS), MS diagnosed, newly diagnosed with MS who have not yet begun treatment (MS Naïve), established MS (>1 year), other neurologic disorders (OND), e.g., Alzheimer's disease, ataxia, Bell's palsy, cerebellar ataxia, cerebral bleed, cervical radiculopathy, Charcot-Marie tooth disease, CNS Lupus, dizziness/pituitary, drug-induced movement disorder, drug-induced tremor, dystonia, epilepsy, essential tremor, Huntington's disease, hydrocephalus, median neuropathy, meningioma, meningitis, migraine, Parkinson's disease, peripheral neuropathy, pituitary adenoma, pseudotumor cerebri, RLS, seizures, spasmodic torticollis, stroke, tension headache, Tourette's syndrome, transient ischemic attack, tremor, and trigeminal tremor. For some comparator groups, ONDs can be grouped by those typically considered inflammatory (OND-I) and those typically considered non-inflammatory (OND-NI).

Because a comparator group can include data from multiple individuals, as will be recognized by one of ordinary skill in the art, it is expected that the expression values of biomarkers in biological samples obtained from different individuals in the same comparator group might differ. As such, identification of a reference ratio for a particular gene pair can be made with reference to a “threshold reference ratio” for the gene pair within the comparator group. In some embodiments, for example, the threshold expression ratio could be a median, an average, a value based on statistical analysis of the distribution of ratios of expression levels of the gene pair within the comparator group, or another threshold value, e.g., top value in the group, second highest value in the group, third highest value in the group, etc.

In some embodiments, the reference comprises a reference ratio calculated using a standard sample containing standard biomarker amounts, which can be analyzed in the same manner or even concurrently with the biological sample. In some embodiments, the reference comprises ratio values, such as standard threshold values. Such values can be published in a format useful for the practitioner, such as in a list, table, database, or incorporated into a software or system for use in connection with the presently-disclosed subject matter. Such values can in some cases be based, for example, on information obtained from a comparator group.

Ratios of interest, or ratios of gene pairs that are useful for characterizing MS, have the ability to distinguish to groups, e.g., MS group and health control group. Table B includes examples of ratios of interest for MS vs. healthy control (CTRL) and MS vs. other neurologic disorders (OND). In this regard, an auto-immune disease can be characterized based on a difference in the ratios of the expression values of at least two genes in a biological sample from the subject as compared to a reference ratio.

In some embodiments, it can be useful to compare one or more subject ratios to one or more first reference ratios, e.g., from a first comparator group, and also to compare the one or more subject ratios to one or more second reference ratios, e.g., from a second comparator group. Such a multi-tiered approach can improve the efficacy of the characterization of the MS, as will be explained further in the Examples section.

Characterizing can include providing a diagnosis, prognosis, and/or theragnosis of an auto-immune disease in a subject.

“Making a diagnosis” or “diagnosing,” as used herein, are further inclusive of making a prognosis, which can provide for predicting a clinical outcome (with or without medical treatment), selecting an appropriate treatment (or whether treatment would be effective), or monitoring a potential auto-immune disease, based on calculated ratios of expression levels of genes. Diagnostic testing that involves treatment, such as treatment monitoring or decision making can be referred to as “theranosis.” Further, in some embodiments of the presently disclosed subject matter, multiple determinations of ratios of expression levels of genes over time can be made to facilitate diagnosis (including prognosis), evaluating treatment efficacy, and/or progression of a potential auto-immune disease or auto-immune disease. A temporal change in one or more ratios can be used to predict a clinical outcome, monitor the progression of the condition, and/or efficacy of administered therapies. In such an embodiment for example, one could observe a change in a particular ratio in a biological sample over time during the progression of a condition and/or during the course of a therapy.

The presently disclosed subject matter further provides in some embodiments a method for theranostic testing, such as evaluating progression of a condition and/or treatment efficacy in a subject. In some embodiments, the method comprises providing a series of biological samples over a time period from the subject; determining expression values of at least two genes in each of the biological samples; calculating one or more ratios of the expression values of the at least two genes for each of the biological samples; and determining any measurable change in the ratios in each of the biological samples from the series to thereby evaluate progression of the condition and/or treatment efficacy.

Any changes in the ratios, and changes in the ratios relative to references, over the time period can be used to make a diagnosis, predict clinical outcome, determine whether to initiate or continue the therapy, and whether a current therapy is effectively.

The phrase “determining the prognosis” as used herein refers to methods by which the skilled artisan can predict the course or outcome of a condition in a subject. The term “prognosis” can refer to the ability to predict the course or outcome of a condition with up to 100% accuracy, or predict that a given course or outcome is more or less likely to occur based on the ratios of expression values of genes of interest. The term “prognosis” can also refer to an increased probability that a certain course or outcome will occur; that is, that a course or outcome is more likely to occur in a subject when compared to individuals in a comparator group. For example, in individuals exhibiting subject ratios-of-interest that are higher than reference ratio-of-interest, the chance of a given outcome (e.g., MS diagnosis) may be very high. In certain embodiments, a prognosis is about a 5% chance of a given expected outcome, about a 7% chance, about a 10% chance, about a 12% chance, about a 15% chance, about a 20% chance, about a 25% chance, about a 30% chance, about a 40% chance, about a 50% chance, about a 60% chance, about a 75% chance, about a 90% chance, or about a 95% chance.

The skilled artisan will understand that associating a prognostic indicator with a predisposition to an adverse outcome can be performed using statistical analysis. For example, subject ratios that are higher than reference ratios in some embodiments can signal that a subject is more likely to suffer from an auto-immune disease than subjects with ratios that are substantially equal to reference ratios, as determined by a level of statistical significance. Statistical significance is often determined by comparing two or more populations, and determining a confidence interval and/or a p value. See, e.g., Dowdy and Wearden, Statistics for Research, John Wiley & Sons, New York, 1983, incorporated herein by reference in its entirety. Exemplary confidence intervals of the present subject matter are 90%, 95%, 97.5%, 98%, 99%, 99.5%, 99.9% and 99.99%, while exemplary p values are 0.1, 0.05, 0.025, 0.02, 0.01, 0.005, 0.001, and 0.0001. When performing multiple statistical tests, p values can be corrected for multiple comparisons using techniques known in the art.

Further with respect to the methods of the presently disclosed subject matter, a preferred subject is a vertebrate subject. A preferred vertebrate is warm-blooded; a preferred warm-blooded vertebrate is a mammal. A mammal is most preferably a human. As used herein, the term “subject” includes both human and animal subjects. Thus, veterinary therapeutic uses are provided in accordance with the presently disclosed subject matter.

As such, the presently disclosed subject matter provides for the diagnosis of mammals such as humans, as well as those mammals of importance due to being endangered, such as Siberian tigers; of economic importance, such as animals raised on farms for consumption by humans; and/or animals of social importance to humans, such as animals kept as pets or in zoos. Examples of such animals include but are not limited to: carnivores such as cats and dogs; swine, including pigs, hogs, and wild boars; ruminants and/or ungulates such as cattle, oxen, sheep, giraffes, deer, goats, bison, and camels; and horses. Also provided is the treatment of birds, including the treatment of those kinds of birds that are endangered and/or kept in zoos, as well as fowl, and more particularly domesticated fowl, i.e., poultry, such as turkeys, chickens, ducks, geese, guinea fowl, and the like, as they are also of economic importance to humans. Thus, also provided is the treatment of livestock, including, but not limited to, domesticated swine, ruminants, ungulates, horses (including race horses), poultry, and the like.

The presently-disclosed subject matter further includes kits and devices useful for detecting and/or determining expression levels of at least two genes in a biological sample.

The kits of the presently-disclosed subject matter can include primer pairs for determining expression levels of at least two genes, which can be useful for calculating ratios as disclosed herein. In some embodiments, the kit includes primer pairs for determining expression levels of at least two genes represented by SEQ ID NOs: 1-47. In some embodiments, the kit includes primer pairs for determining expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes represented by SEQ ID NOs: 1-47. In some embodiments, the kit includes primer pairs for determining expression levels of at least two genes corresponding to those set forth in Table A. In some embodiments, the kit includes primer pairs for determining expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes corresponding to those set forth in Table A. In some embodiments, the kit includes primer pairs for determining expression levels of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP. In some embodiments, the kit includes primer pairs for determining expression levels of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. In some embodiments, the kit includes primer pairs for determining expression levels of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, or 34 genes corresponding to those set forth in Table B.

The devices of the presently-disclosed subject matter can include a probe for selectively binding each of at least two gene expression products to detect at least two genes, which can be useful for determining expression levels of the genes and for calculating ratios as disclosed herein. Such probes can selectively bind the gene products, for example, by hybridization of the probe and a nucleotide gene product. In some embodiments, the device includes probes for detecting each of at least two genes represented by SEQ ID NOs: 1-47. In some embodiments, the device includes probes for detecting each of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes represented by SEQ ID NOs: 1-47. In some embodiments, the device includes probes for detecting each of at least two genes corresponding to those set forth in Table A. In some embodiments, the device includes probes for detecting each of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 genes corresponding to those set forth in Table A. In some embodiments, the device includes probes for detecting each of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP. In some embodiments, the device includes probes for detecting each of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. In some embodiments, the device includes probes for detecting each of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, or 34 genes corresponding to those set forth in Table B.

Some of the gene sequences disclosed herein are cross-referenced to GENBANK® accession numbers. The sequences cross-referenced in the GENBANK® database are expressly incorporated by reference as are equivalent and related sequences present in GENBANK® or other public databases. Also expressly incorporated herein by reference are all annotations present in the GENBANK® database associated with the sequences disclosed herein. Unless otherwise indicated or apparent, the references to the GENBANK® database are references to the most recent version of the database, as of the filing date of this application.

While the terms used herein are believed to be well understood by one of ordinary skill in the art, definitions are set forth to facilitate explanation of the presently-disclosed subject matter.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the presently-disclosed subject matter belongs. Although any methods, devices, and materials similar or equivalent to those described herein can be used in the practice or testing of the presently-disclosed subject matter, representative methods, devices, and materials are now described.

Following long-standing patent law convention, the terms “a”, “an”, and “the” refer to “one or more” when used in this application, including the claims. Thus, for example, reference to “a cell” includes a plurality of such cells, and so forth.

Unless otherwise indicated, all numbers expressing quantities of ingredients, properties such as reaction conditions, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about”. Accordingly, unless indicated to the contrary, the numerical parameters set forth in this specification and claims are approximations that can vary depending upon the desired properties sought to be obtained by the presently-disclosed subject matter.

As used herein, the term “about,” when referring to a value or to an amount of mass, weight, time, volume, concentration or percentage is meant to encompass variations of in some embodiments ±20%, in some embodiments ±10%, in some embodiments ±5%, in some embodiments ±1%, in some embodiments ±0.5%, and in some embodiments ±0.1% from the specified amount, as such variations are appropriate to perform the disclosed method.

As used herein, ranges can be expressed as from “about” one particular value, and/or to “about” another particular value. It is also understood that there are a number of values disclosed herein, and that each value is also herein disclosed as “about” that particular value in addition to the value itself. For example, if the value “10” is disclosed, then “about 10” is also disclosed. It is also understood that each unit between two particular units are also disclosed. For example, if 10 and 15 are disclosed, then 11, 12, 13, and 14 are also disclosed.

The presently-disclosed subject matter is further illustrated by the following specific but non-limiting examples. The following examples may include compilations of data that are representative of data gathered at various times during the course of development and experimentation related to the present invention.

EXAMPLES Example 1 Gene Expression Patterns in Distinct Neurologic Diseases

Expression patterns of a common set of genes assayed using a common platform in control subjects and subjects with different neurologic conditions, including autoimmune diseases, were measured. Expression levels of individual genes were determined by quantitative RT-PCR by normalization to GAPDH expression levels. A heatmap was employed to depict those genes differentially expressed in individual disease cohorts relative to the control cohort, P <0.05 (after Bonferroni correction for multiple testing) (FIG. 1). Ratios of expression levels of individual genes in the indicated disease cohort relative to the control cohort were calculated and depicted within each colored box. Each disease exhibited an underlying unique pattern of gene expression. However, these profiles were sufficiently overlapping to prohibit accurate discrimination of one disease from another disease using the expression profile alone. For example, LLGL2, RANGAP1, ACTB, and POU6F1 were under-expressed in 4, 3, 4, and 4 of 5 different conditions, respectively. In contrast, other genes, e.g., ANAPC1 in Parkinson's disease, EXT2 and FOS in TM, HRAS in NMO, were only differentially expressed in a single disease cohort. Overall, individual genes were either over-expressed, e.g. B2M, CD55, PMAIP1, or under-expressed, e.g. LLGL2, RANGAP1, ACTB, across multiple disease cohorts. Thus, each gene was differentially expressed in at least one disease cohort relative to the CTRL cohort. However, each individual disease cohort did not possess a unique expression profile distinguishing it from all other disease cohorts.

Discrimination of MS from Homogeneous Comparator Groups: Identification of an Optimum Panel of Gene Expression Ratios

Healthy control subjects, subjects with MS, and subjects with other inflammatory neurologic disorders (OND-I), and subjects with neurologic disorders typically considered non-inflammatory (OND-NI) were recruited from multiple U.S. and European sites (Table 1-A and Table 1-B). Demographic characteristics of the different disease groups, MS, OND-I, or OND-NI were matched to the CTRL cohort (compilation of patient characteristics data not shown). Subjects with MS included subjects with clinically isolated syndrome (CIS), newly diagnosed MS subjects who were treatment naïve and subjects with established disease (>1 yr duration) on different therapies. Expression levels of test and control genes in blood were determined by quantitative reverse transcription polymerase chain reaction (RT-PCR) (Table 1-C).

TABLE 1-A Characteristics of Subjects Sites Nashville U.S.* Europe** MULTIPLE SCLEROSIS (total) 84 81 80 CIS 14 10 Treatment naïve 30 4 55 Established disease (on meds) 40 77 15 OND-I (total) 1 85 Acute disseminated 4 encephalomyelitis Bell's Palsy 3 CNS lupus 2 Guillaine Barre 4 Myasthenia Gravis 3 Neuromyelitis optica 27 Optic neuritis 1 1 Transverse myelitis 41 OND-NI (total) 1 128 Alzheimer's 6 Cerebral ataxia 2 Cerebral bleed 2 Cervical radiculopathy 6 Drug-induced movement 1 disorder Dystonia 1 Epilepsy 1 4 Essential tremor 9 Huntington's disease 1 Hydrocephalus 1 Median Neuropathy 2 Meningioma 1 Migraine 30 Parkinson's 3 0 Peripheral Neuropathy 1 Pseudotumor 3 Restless Leg Syndrome 1 Seizures 6 Spasmodic torticollis 1 Stroke 18 Tourette's Syndrome 1 Transient Ischemia 1 CONTROLS 48 61 *six additional sites in U.S.: MA, MD, NY, SC, AZ, TX, CA, samples from sites in MS, MD, NY, AZ, and CA were obtained through the Accelerated Cure Project. **Denmark, Netherlands

TABLE 1-B Demographic characteristics of the different subject populations. ETHNIC- GENDER ITY (%, AGE P* (% F) P C/AA/As/H)** P MS 43 ± 10 NS 76 NS 80/20/0/0 NS OND-NI 46 ± 10 NS 67 NS 68/26/3/1 NS OND-I 46 ± 10 NS 68 NS 67/33/0/0 NS CTRL 41 ± 11 77 71/22/3/3 *P calculated by Student's T-test (Age) or Fisher's exact test. NS: P > 0.05. **C, Caucasian; AA, African American; As, Asian; H, Hispanic.

TABLE 1-C Gene probes on TLDA plate ABR EPHX2 OAS1 ACTB EXT2 ORC1L ACTR1A FOS PGK1 ADAMTSL4 FOSL1 PMAIP1 ANAPC1 GAPDH POU6F1 APOBEC3F GATA3 RANGAP1 ASL GNB5 SC65 B2M GSTM4 SPIB BRCA1 HLA-DRA TAF11 CD55 HRAS TBP CDH1 IFI27 TGFBR2 CDKN1B IL11RA TP53 GAPDH JUN TXK CHEK2 KRAS GNB5 CSF3R LLGL2 TP53 CTSS NRAS

A search algorithm was employed to identify those ratios of gene expression levels in which the greatest number of subjects in the test group possessed a ratio value greater than the highest ratio value in the comparator group. A second algorithm was employed to perform permutation testing of one subject group to identify the optimum set of discriminatory ratios.

It was reasoned that examination of expression levels of ratios of genes rather than individual genes would serve the following purposes. First, calculation of ratios normalized for differences in mRNA or cDNA template quantity and quality among different samples. Second, they obviated the need for inclusion of a ‘housekeeping’ gene in the analysis and the assumption that expression levels of ‘housekeeping’ genes did not vary among different subject populations. Third, comparisons of ratios or combinations of ratios may more accurately identify cellular phenotypes that may contribute to disease. For example, a ratio containing one gene in the numerator that is over-expressed in the test group relative to the comparator group and one gene in the denominator that is under-expressed in the test group relative to the comparator group should produce a greater ratio value difference between individuals in the two groups than a single expression value. A point system was employed to award one point to a subject if a ratio value of the test subject was greater than the ratio values of all subjects in the comparator group.

This approach was applied to determine how accurately it would distinguish subjects with MS from healthy control subjects. First, ratios capable of discriminating MS subjects from control subjects were identified. The single ratio with the greatest discriminatory power was ANAPC1/CHEK2 (FIG. 2 a). Fifty % of MS subjects achieved a ratio value higher than all the CTRL subjects and were awarded one point. Second, those ratios that identified fewer than 20% of MS subjects were eliminated. Third, since many ratios identified the same MS subjects, another reduction was performed to preserve only one ratio with this characteristic. A total of 8 ratios remained after this minimization process (FIG. 2 b). Using the point system, the combination of these 8 ratios positively identified 97% of MS subjects and eliminated 100% of CTRL subjects (FIG. 2 c). The score distribution was 0-6 for MS subjects and 0 for CTRL subjects (FIG. 2 d).

Discrimination of MS from Homogeneous Comparator Groups: Validation and Analysis

The analyses depended upon determination of multiple ratios, which may create Type 1 errors. Various methods are available to correct for false discovery rates. Rather than relying upon these methods, which all make underlying assumptions, a second evaluation was performed using an independent cohort of 40 new MS subjects and 40 new CTRL subjects to validate results obtained from the initial training set. These subjects were recruited separately and the PCR analyses were performed separately. The same ratio values were used, as defined from the original CTRL and MS test set to award points to subjects in the validation cohort. All 40 controls were awarded a score of 0 while 4% of MS subjects received a score of 0. The remaining 96% of MS subjects achieved a score of 1-6 and the distribution of scores was similar to that observed in the training set (FIG. 2 e). Taken together, this demonstrates that results obtained in the training set can be replicated in an independent cohort of CTRL and MS subjects.

The point system was applied to OND-I and OND-NI subjects. In contrast to CTRL subjects, 90% of OND-I and 59% of OND-NI subjects scored ≧1 (FIG. 2 f). Scores among subjects with CIS were compared, with newly diagnosed MS not yet on medications, and with established MS on different medications. Scores did not differ significantly among these three groups (FIG. 2 g). Also compared were scores within the MS group as a function of geographic origin. Scores also did not vary significantly among MS subjects from different geographic sites (FIG. 2 h). Thus, subjects with CIS or subjects after their initial diagnosis of MS had a similar mean score to subjects with established MS on therapies. However, a high percentage of subjects with other neurologic conditions, especially inflammatory neurologic conditions, also scored ≧0 in this analysis. Given its extremely high specificity and relatively low sensitivity, embodiments of this test have greater application to exclude an individual from the diagnosis of MS rather than to establish a diagnosis of MS.

Further, follow-up clinical information on 8 CIS subjects >2 yr. after the initial consent and blood draw were able to be obtained. Of these subjects, the 7 CIS subjects who achieved a score >0 in the analysis now have documented MS. The 1 CIS subject who achieved a score of 0 does not have a documented case of MS.

NMO and TM are inflammatory neurologic diseases that scored positive in the analysis. Therefore, it was determined whether a similar approach could be employed to discriminate MS from TM and MS from NMO. A series of ratios were identified that, when combined using the point system, were able to discriminate TM from MS and NMO from MS with similar overall accuracy to the MS and CTRL comparisons (FIG. 3). Thus, using the approach, it was possible to distinguish MS from TM and MS from NMO with a similar degree of accuracy as obtained for the comparison of MS to CTRL. However, since each disease possessed a unique signature, it was necessary to employ separate combinations of ratios to accurately distinguish MS from NMO and MS from TM.

Above results demonstrate it is possible to distinguish MS from either a control cohort or even a related inflammatory disease cohort if the disease cohort is a single disease.

Next, it was determined whether MS could be discriminated from Parkinson's disease, a disorder typically considered non-inflammatory. To test this hypothesis in it was determined if subjects with Parkinson's disease (N=24) segregated from MS (N=182) and from CTRL (N=109) using the ratio and point system. Ten (10) ratios capable of discriminating 97% of MS subjects from 100% of Parkinson's subjects and 9 ratios capable of discriminating 88% of Parkinson's patients from 100% of CTRL subjects were identified (FIG. 4). These results demonstrate that subjects with Parkinson's disease express unique gene expression signatures in blood distinguishing them from CTRL and MS subjects.

Discrimination of MS from Heterogeneous Comparator Groups

Next, it was determined whether MS could be distinguished from more heterogeneous groups of subjects. To do so, subjects with neurologic conditions typically considered as inflammatory (other neurologic disorders-inflammatory, OND-I in Table 1-B) were combined into one group. Subjects with neurologic conditions typically considered non-inflammatory (other neurologic disorders-non-inflammatory, OND-NI, OND in Table 1-B) were combined into a second group. A third group consisting of CTRL+OND-I+OND-NI subjects (ALL) was prepared. The 15 best ratios were determined using permutation testing for each comparison. Overall, comparison of MS to these heterogeneous comparator groups resulted in a marked reduction in overall discrimination ability (FIG. 5). It was concluded that a binary comparison such as this exhibits much reduced accuracy as the heterogeneity of the comparator group is increased.

Discrimination of MS from OND-I: Identification of Optimum Panels of Gene Expression Ratios

For additional analysis, OND-I was combined into one group of non-MS inflammatory neurologic disorders and investigated the ability of the approach to discriminate this combination of diseases from MS. The conditions were relaxed somewhat to identify ratios with the ability to detect 0 or 1 non-MS subjects. The best results were obtained with 10 ratios (FIG. 6 a). The combination of which identified 86% of MS subjects with a score >0 and only 8% of OND-I subjects with a score >0 (FIG. 6 b). Scores ranged from 0-7 for MS subjects and 0-1 for OND-I subjects (FIG. 6 c).

Discrimination of MS from OND-I: Validation and Analysis

Additional analyses were performed with 40 new MS subjects and 40 new OND-I subjects (20 NMO and 20 TM) not included in the training set. In the validation set, 88% of MS subjects achieved a score ≧1 and 12% of OND-I subjects achieved a score of 1 (FIG. 6 d), which was similar to the score distribution observed in the training set. The mean scores among subjects with CIS were determined, subjects with newly diagnosed MS prior to onset of therapies, and subjects with established MS on therapies using the 10 ratios identified above. Mean scores were significantly higher in the CIS and MS-naïve groups than in the MS group with established disease (FIG. 6 e). Mean scores based upon geographic origins of MS subjects were also determined. Subjects from Nashville and Europe had mean scores significantly greater than U.S. subjects from locations other than Nashville (FIG. 6 f). These results are consistent with results comparing CIS, MS-naïve, and MS-established. The majority of subjects from U.S. sites outside Nashville had established MS and were on therapies (76 of 80 subjects) while all European subjects were either CIS or newly diagnosed MS subjects not yet on therapies (N=101). The Nashville site also provided more samples with established disease (N=37) compared to CIS or treatment naïve MS (N=16) (P <0.0001, Chi-squared test for independence among three geographic locations). The distribution of scores in the CIS and newly diagnosed MS group was also higher than that found in the established MS group. Greater than 50% of subjects with established MS achieved scores of 0 or 1 while 48% of CIS and newly diagnosed MS subjects achieved scores ≧3 (FIG. 6 g). Thus, subjects with CIS, newly diagnosed MS, and established MS from different geographic sites can be distinguished from subjects with OND-I with reasonable accuracy based upon gene expression profiles in whole blood.

Discrimination of MS from OND-NI: Identification of Optimum Panels of Gene Expression Ratios

Next, gene expression differences between MS and OND-NI subjects were compared, which included Parkinson's disease, essential tremors, migraines, and strokes. The same search strategy used to compare MS and OND-I subjects was employed and identified 10 expression ratios to construct the point system. ABOBEC3F, CSF3R, and ANAPC1 were each in the numerators of two ratios and TAF11 was in the denominator of two ratios. Each ratio alone detected >10% of MS subjects relative to OND-NI subjects (FIG. 7 a). Combining ratios using the point system improved overall ability to discriminate MS subjects from OND-NI subjects (FIG. 7 b). Using the point system, 79% of MS subjects achieved a score ≧1 and 91% of OND-NI subjects achieved a score of 0, 9% achieved a score of 1 (FIG. 7 c).

Discrimination of MS from OND-NI: Validation and Analysis

Additional analyses were performed with 40 new MS subjects and 40 new OND-NI subjects not included in the training set as outlined above. In the validation set, 88% of MS subjects achieved a score ≧1 (FIG. 7 d), which was a similar frequency to that observed in the training set, and 90% of OND-I subjects achieved a score of 0, 10% achieved a score of 1. As above, mean scores of subjects with CIS, newly diagnosed MS and established MS were determined and these were not statistically different among the three MS groups (FIG. 7 e). Similarly, mean scores of MS subjects from different geographic sites were not statistically different (FIG. 7 f). Using the point system, ˜80% of MS subjects achieved a score ≧1 and 9% of OND-NI subjects achieved a score=1 in the test set. These results demonstrate that expression in whole blood of a different set of gene ratios discriminated subjects with MS from subjects with OND-NI with reasonable accuracy.

All comparisons in these analyses were binary. Therefore, exclusion of a specific disorder by the analysis may be more accurate than inclusion of a specific disorder (see flow chart).

-   -   Flow Chart: Tiered approach using expression ratios to determine         probability of the presence or absence of MS

Analysis 1: MS versus control

-   -   score=0: >95% probability subject does not have MS     -   score ≧1: move to analysis 2

Analysis 2A: MS versus OND-I

-   -   score=0: ˜8-fold greater likelihood of OND-I than MS     -   score ≧1: ˜8-fold greater likelihood of MS than OND-I

Analysis 2B: MS versus OND-NI

-   -   score=0: ˜8-fold greater likelihood of OND-NI than MS     -   score ≧1: ˜8-fold greater likelihood of OND-NI than MS

Analysis 3A: MS versus NMO

-   -   score=0: ˜90% probability subject does not have MS     -   score ≧1: ˜90% probability subject does not have NMO

Analysis 3B: MS versus TM

-   -   score=0: ˜90% probability subject does not have MS     -   score ≧1: ˜90% probability subject does not have TM

Thus, a score of 0 in the MS versus CTRL test decreased the probability that a subject had MS. A second analysis comparing MS to OND-I and MS to OND-NI would be interpreted similarly. Scores of 0 decreased the probability of MS and favored the probability of OND-I or OND-NI, respectively. Finally, specific inflammatory neurologic disorders, NMO or TM, were distinguished from MS with high degrees of accuracy. Thus, results from this single platform can be analyzed in a tiered approach to provide meaningful disease classification.

Discussion

Although the focus was on MS and other inflammatory and non-inflammatory neurologic disorders, the results support the notion that this approach could be applicable to an array of diseases. First, discrimination between MS and healthy controls or subjects with individual diseases can be achieved with a relatively high degree of accuracy. However, subjects with OND-I and OND-NI also scored positive in MS-CTRL comparisons. As such, this single comparison has greater utility as an exclusionary test rather than a test of MS inclusion. Second, it is possible to discriminate MS from groups of diseases, such as inflammatory or non-inflammatory neurologic diseases, and validate results in independent cohorts, although overall accuracy is somewhat compromised. Third, discrimination of MS from a diverse comparator group including CTRL, OND-I, and OND-NI causes a further reduction in overall accuracy. Nevertheless, a score >0 in this analysis is highly predictive of the presence of MS. Fourth, it is possible to identify small numbers of ratios with high degrees of discriminatory power whose accuracy can be validated in independent cohorts analyzed separately.

One interpretation of the results is that many individual diseases express unique but overlapping gene expression signatures in whole blood. Given the attention paid to analyses of autoimmune diseases, it is not surprising that inflammatory neurologic diseases such as NMO and TM also express unique gene expression signatures. Perhaps somewhat surprising is that Parkinson's disease, a disorder typically considered non-inflammatory, also possesses a unique gene expression signature distinguishing it from both CTRL and MS. Implications may be that the immune system can sense specific neurologic damage caused by Parkinson's via responses to cytokine mediators, adhesion molecules, neurotransmitters, or other mediators read by immune cells. Alternatively, genetic risk factors associated with Parkinson's disease may contribute to altered gene expression signatures by either direct or indirect mechanisms.

Mechanisms underlying gene expression differences among study groups or relationships to MS disease mechanism are not altogether clear. However, defects in DNA damage repair, cellular responses to DNA damage, and regulation of cell cycle progression and arrest are common properties of lymphocytes in certain autoimmune diseases, including MS, and ANAPC1, CHEK2, CDKN1B, ACTB, FOSL1, LLGL2, and NRAS encode proteins playing key roles in these fundamental cellular processes²³⁻²⁷. These genes are highly represented in the ratios used to distinguish MS from comparator groups. Genes, such as ADAMTSL4, B2M, IL11RA, TXK and POU6F1, encode proteins playing key functions in regulating cells of both innate and adaptive arms of the immune system^(28, 29). As such, alterations in expression of these genes may contribute to pathogenesis of MS or may represent an altered response by the immune system to MS pathogenesis.

The follow-up analysis of CIS patients supports the idea that initial scores >0 will correlate with progression to MS. Future longitudinal studies are planned to better evaluate utility of these tests in this setting. Further, the binary analysis is also predicated on the fact that MS is best represented by a single set of gene expression ratios and this may not be the case. Additional analyses, such as analyses of gene expression ratios in multi-dimensional space, will address this possibility. Several different combinations of gene expression ratios were identified, which performed equivalently in their ability to discriminate among subject groups. In conclusion, these minimally invasive and relatively inexpensive tests may have utility to either exclude the diagnosis of MS or to contribute to establishing a diagnosis of MS.

Materials and Methods

Patients.

Blood samples in PAXgene tubes were obtained from patients with a) clinically isolated syndrome (CIS), b) an initial diagnosis of MS before onset of therapy, and c) established relapsing-remitting MS on medication. Blood samples were also obtained from healthy control subjects (CTRL) and subjects with different inflammatory (OND-I) or non-inflammatory (OND-NI) neurologic conditions. MS samples were obtained from a total of 9 different sites in the U.S. and Europe. Samples from subjects with OND-I and OND-NI were obtained from 7 sites in the U.S. CTRL samples were obtained from 3 U.S. sites. Inclusion criteria for MS and other neurologic conditions were diagnosis by a neurologist using established methods and ability to provide informed consent, thus providing an un-biased study cohort. Age, race and gender were not statistically different among the different study groups. Time of the blood draw, e.g. morning/afternoon clinics, was also not statistically different among the different study groups. Relevant institutional review board approval from all participating sites was obtained.

Procedures.

Total RNA, purified using Qiagen's isolation kits by standard protocols, was reverse-transcribed using SuperScript III (Invitrogen). A TaqMan Low Density Array (TLDA) was designed to analyze expression levels of 44 genes previously identified from the microarray analysis and of 4 “housekeeping” genes in 300 ng cDNA per sample. Patient diagnosis was blinded for all experimental procedures. Relative expression levels were determined directly from the observed threshold cycle (C_(T)), the cycle number at which fluorescence generated within reactions crosses an assigned threshold reflecting the point where sufficient amplicons have accumulated to be statistically significant above baseline. Linear expression values were determined using the formula, 2^((40-CT)).

Identification of Discriminatory Gene Expression Ratios.

A computational algorithm was designed to identify the most discriminatory combinations of ratios²². All possible gene expression ratios were computed (e.g. ACTR1A/BRCA1, TAF11/ACTR1A, etc). To analyze individual results, R_(i,j) ^(control) was used to denote the i^(th) ratio for the j^(th) control and let R_(i,k) ^(MS) was used to denote the i^(th) ratio for the k^(th) MS patient. Here, j=1, . . . , N_(control) and k=1, . . . , N_(MS), where N_(control) equals the total number of controls and N_(MS) equals the total number of MS patients in the data set. The second largest member of each data set of ratios was calculated first by {R_(i,1) ^(control), R_(i,2) ^(control), . . . , R_(i,N) _(control) ^(control)}, and designated R_(i) ⁽²⁾. This was then applied to the MS data set {R_(i,1) ^(MS), R_(i,2) ^(MS), . . . , R_(i,N) _(MS) ^(MS)}. C_(i) was used to designate the number of MS set of ratios larger than R_(i) ⁽²⁾ such that 0≦C_(i)≦N_(MS). This process was repeated for each possible ratio. The ratio that produced the largest C_(i) was selected as the discriminator of the two sets. This process was repeated using all possible ratios. Although more than one optimal ratio could be identified for each number of components queried, only one discriminator has been presented for each combination. Ratios were included only if >20% of subjects within the MS group had expression values greater than all subjects in the CTRL group. A scoring system was developed to combine multiple ratios. To do so, subjects were assigned one point for each ratio in which their expression value was higher than the highest expression value within the CTRL subject group. By this approach, it was also possible to relax search criteria by setting cutoffs to the second highest expression ratio, third highest expression ratio, etc., of the comparator subject group. Using these relaxed criteria, an individual was awarded one point if the value of their expression ratio was higher than the second or third, etc., highest expression value of individuals in the comparator group, respectively. These combined ratios established a score discriminating the MS group from comparator groups.

Search Algorithm for Best Ratios.

Let D denote the set of 44 gene-expression levels associated with the disease group and C denote the set of gene-expression levels associated with the control group. For example, when D is the set of MS patients, then D is a set of 182 44-tuples; if C is associated with the Controls, then C is a set of 51 44-tuples. The algorithm that searches for the “best” set of gene ratios is the following:

-   -   80% of the control group was randomly selected and compared to         the disease group in the following manner. Gene-expression level         ratios were formed for elements in D and C. For each ratio, the         number of elements in the disease group that were larger than         the largest ratio in the control group was computed. The top 500         ratios that separate elements in D and C were saved. This         calculation was repeated 200 times resulting in a set of 200         subsets of ratios (each subset having 500 ratios).     -   The 500 subsets were processed to identify the smallest number         of ratio, R={r₁, r₂, . . . , r_(n)}, that produced the maximum         of separation of D and C. Associated with each of the ratios in         R, there were threshold values, T={t₁, t₂, . . . , t_(n)}, which         corresponded to the highest value in the control group for each         of the ratios in R.     -   For each member of the disease group D, the ratios in R were         computed, {α₁, α₂, . . . , α_(n)}. If α₁≧t₁, then the ratio a         was assigned 1; otherwise, it was assigned a 0. In this way, an         n-tuple of 1's and 0's was generated for each member of D. For         example, if n=6, then a typical 6-tuple would be {1,1,0,0,1,0}.         This meant that this individual in the disease group would have         3 ratios that exceeded the corresponding ratios in the control         group.     -   Lastly, the percentage of members in the disease group that had         nonzero n-tuples was calculated. The larger the percentage, the         better the separation of D and C.

Statistical Analysis

The Welch's corrected T-test not assuming equal variances was used to calculate P values in two-way comparisons. The Chi-squared test for independence was used to calculate P values in three-way comparisons. The Bonferroni method was employed to correct for multiple testing³⁰.

Example 2 Using Biomarkers to Predict Progression from Clinically Isolated Syndrome to Multiple Sclerosis

Patients.

A total of 562 subjects were included in the study: 199 with clinically definite MS, 203 with OND segregated into 84 OND-I subjects and 119 OND-NI subjects, 114 healthy control subjects and 46 subjects whose blood sample was obtained at the time of their CIS but who now have progressed to clinically definite MS, CIS→MS (Table 2-A). MS patients were divided into two additional categories: those at their initial diagnosis of MS but before initiation of therapies; MS-naïve, and those ≧1 year after diagnosis of MS and on different therapies; MS-established. The overall laboratory and analytic processes are summarized in FIG. 8.

TABLE 2-A Demographic characteristics of the different subject populations. ETHNIC- GENDER ITY (%, # AGE P* (% F) P C/AA/As/H)** P MS 199 43 ± 10 NS 76 NS 80/20/0/0 NS OND-I 84 46 ± 10 NS 68 NS 67/33/0/0 NS OND-NI 119 46 ± 10 NS 67 NS 68/26/3/1 NS CTRL 114 41 ± 11 77 71/22/3/3 CIS →MS 46 35 ± 6  NS 72 NS 82/14/4/0 NS MS = MS-treatment naïve (N = 85), MS with established disease on medications (N = 114), OND-I = other inflammatory neurologic disorders, acute disseminated encephalomyelitis (N = 4), Bell's Palsy (N = 3), CNS lupus (N = 2), Guillaine Barre (N = 4), Myasthenia Gravis (N = 3), Neuromyelitis optica (N = 26), Optic neuritis (N = 1), Transverse myelitis_(N = 41), OND-NI = other non-inflammatory neurologic disorders, Alzheimer's (N = 6), cerebral ataxia (N = 2), cerebral bleed (N = 2), cervical radiculopathy (N = 6), drug-induced movement disorder (N = 1), dystonia (N = 1), epilepsy (N = 4), essential tremor (N = 9), Huntington's disease (N = 1), hydrocephalus (N = 1), median neuropathy (N = 2), meningioma (N = 1), migraine (N = 30), Parkinsons (N = 23), peripheral neuropathy (N = 1), pseudotumor (N = 3), restless leg syndrome (N = 6), seizures (N = 9), stroke (N = 10), CIS →MS: subjects who had clinically isolated syndrome at the time of the blood draw who have developed clinically definite MS. U.S. sites: TN, MA, MD, NY, SC, AZ, TX, CA, samples from sites in MS, MD, NY, AZ, and CA were obtained through the Accelerated Cure Project, European sites: Denmark, Netherlands *P calculated by Student's T-test²¹ or Fisher's exact test, NS: P > 0.05, calculated relative to CTRL. **C, Caucasian; AA, African American; As, Asian; H, Hispanic.

Transcript Profiles.

The transcript level in blood was determined for each target gene relative to GAPDH in the three study groups, CIS→MS, MS-naïve, MS-established and the CTRL group using TLDA plates. Target genes were selected from previous microarray studies.¹⁷⁻¹⁹ The ratio, log₂, of the expression level of each gene in each study group was calculated relative to CTRL and results are presented in a heatmap. Numerical ratios, log₂, are displayed within each box (FIG. 9 a). Transcript profiles in the three study groups, CIS→MS, MS-naïve, and MS-established were highly dynamic. In the CIS→MS cohort, most genes were significantly over-expressed relative to CTRL. In contrast, the majority of target genes were significantly under-expressed in the MS-established cohort. The MS-naïve cohort was intermediate with an almost equal number of over- and under-expressed genes (FIG. 9 b). Using the student's T test, P-values, log₁₀, were determined comparing each study group cohort to the CTRL cohort (FIG. 9 c). Differences in transcript levels of many genes were highly significant among the different study groups. Of note, the P-value, log₁₀, for PGK1 expression between the CIS→MS cohort and CTRL cohort was −13.3. Similarly, expression differences of LLGL2 was most significant in the MS-naïve cohort, log₁₀=−9.6 and expression differences of POU6F1 was most significant in the MS-established cohort, log₁₀=10.3. One interpretation of these results is that each subject within each of these three disease cohorts, CIS→MS, MS-naïve, and MS-established, has a very similar target gene transcript profile suggesting that each is mediated by a common underlying molecular pathway(s) or event(s). Even though this is a cross-sectional rather than a longitudinal study, a second interpretation of these results is that target gene transcript profiles are highly dynamic as a subject progresses from CIS to clinically definite MS to MS disease of some duration.

Ratioscore Algorithm.

The previously described ratioscore method was used to compute all gene expression ratios and permutation testing to identify the set best able to discriminate the MS cohort, naïve and established combined, from the CTRL cohort⁴⁰. A heatmap was generated to depict which ratios (columns) were positive for each MS subject (rows) (FIG. 10 a). One or more positive ratios produces a score ≧1 making a subject positive for the indicated disease, in this case, MS. A total of 173 of 199 MS subjects (87%) were assigned to the MS category using the ratioscore method and 100% of CTRL subjects were excluded from the MS category. Using these gene expression ratios, data was input from the CIS→MS cohort to determine if these subjects would fall into the MS or CTRL category. As above, a heatmap was constructed to depict which ratios (columns) were positive in each CIS→MS subject (rows). A total of 44 of 46 CIS→MS subjects (96%) were assigned to the MS category using the ratioscore defined for MS (FIG. 10 b).

Using a similar approach, the ratioscore algorithm was used to compute ratios to discriminate MS, combined MS-naïve and MS-established from OND. As above, a heatmap was generated to depict which ratios (columns) were positive for each MS subject (rows) (FIG. 11 a). A total of 140 of 199 MS subjects (70%) were assigned to the MS category using the ratioscore method and 203 of 203 (100%) of OND subjects were excluded from the MS category. As above, using these gene expression ratios, data was input from the CIS→MS cohort to determine if these subjects would fall into the MS or CTRL category. A similar heatmap was constructed to depict which ratios (columns) were positive in each CIS→MS subject (rows). A total of 46 of 46 CIS→MS subjects (100%) fell into the MS category using the ratioscore method (FIG. 11 b).

The rationale for performing this two-tier analysis rather than combining the CTRL and OND subjects into one cohort was that previous studies demonstrated that accuracy was severely compromised. To confirm that this was the case in this analysis the MS cohort was compared to the combined CTRL plus OND cohort and these data were inputted into the ratioscore algorithm. As expected, overall ability to discriminate MS from this combined cohort was compromised. Only 58% of MS subjects were assigned to the MS category while 100% of subjects in the combined CTRL plus OND cohort were excluded from the MS category (FIG. 12 a). When data was input from the CIS→MS cohort, only 28 of 46 subjects (61%) were categorized as MS (FIG. 12 b). Thus, overall accuracy of the ratioscore method was much improved by performing two tiers of analysis, first MS versus CTRL, then MS versus OND.

The OND cohort was also subdivided into OND-I and OND-NI (Table 2-A) and the ratioscore algorithm was repeated to assess how well these sub-groups could be distinguished from MS (FIG. 13 a &13 b). In the OND-I versus MS comparison, 90% of MS subjects were assigned to the MS class and 100% of OND-I subjects were excluded from the MS class. When data was input from the CIS→MS cohort, 46 of 46 subjects (100%) were categorized as MS. In the OND-NI versus MS comparison, 86% of MS subjects were assigned to the MS class and 100% of OND-NI subjects were excluded from the MS class. When data was input from the CIS→MS cohort, 46 of 46 subjects (100%) were categorized as MS. It was conclude that this further subdivision of OND subjects produces only limited improvement in overall accuracy.

Accuracy of Ratioscore and SVM Methods.

A support vector machine (SVM) was also trained with ratios identified by the ratioscore method using 60% of CTRL subjects and 60% of cases (see Methods). SVM was validated with the remaining 40% of CTRLs and cases. Subjects within the CIS→MS cohort were input into the SVM to ascertain if the SVM would identify them as controls or cases. New SVMs were created using 60% of OND, OND-NI, and OND-I cohorts as controls, respectively and 60% of MS subjects as the case cohort. SVMs were validated with the remaining 40% of the respective control cohort and remaining 40% of the case cohort²⁰. As above, subjects within the CIS→MS cohort were input into each SVM to ascertain if the SVM would identify them as controls or cases. Results from the SVM method were compared to results from the ratioscore method by calculating sensitivity and specificity (Table 2-B). Overall, ratioscore and SVM produced comparable sensitivity and specificity in control: case comparisons. More relevant, subjects within the CIS→MS cohort were identified as MS by both methods with a high degree of specificity. Thus, this tiered approach, MS:CTRL then MS:OND, could be employed to predict if a subject with CIS will develop MS with a reasonable level of overall accuracy.

TABLE 2-B Sensitivity and specificity of ratioscore and SVM methods RATIOSCORE SVM sensi- speci- sensi- speci- CONTROL CASE tivity ficity tivity ficity #1 CONTROL MS .87 1.00 0.86 0.93 CONTROL CIS → MS .96 0.95 #2 OND MS 0.70 1.00 0.82 0.78 OND CIS → MS 1.00 1.00 #3 OND-NI MS 0.86 1.00 0.84 0.94 OND-NI CIS → MS 1.00 1.00 #4 OND-I MS 0.90 1.00 0.77 0.93 OND-I CIS → MS 1.00 0.98 Optimum ratios for the ratioscore method were from FIGS. 10, 11 and 13. CIS → MS subject data were inputted and scores computed. For the SVM, 60% of controls and cases were randomly selected for the training set and 40% were used for the validation set. Sensitivity and specificity were calculated for the combined sets. These results defined the SVM. CIS → MS subject data were applied to the SVM and subjects received a score of 0 if assigned to the CONTROL cohort or 1 if assigned to the CASE cohort. Sensitivity was calculated from this output. Sensitivity = # true positives/(# true positives + # false negatives) Specificity = # true negatives/(# true negatives + # false positives)

To summarize, overall transcript profiles in the CIS→MS, MS-naïve, and MS-established were markedly different and these dynamic transitions may reflect differences in underlying etiology. Studying the molecular origins of the robust transcript signature in CIS→MS subjects may produce insights into the origins of MS. In spite of the differences in overall transcript profiles in these three subject groups, ratioscore and SVM methods were able to assign CIS→MS subjects to the MS category with a high degree of accuracy. This is due, in part, to the fact that the ratioscore method does not require that all subjects within these three cohorts representing three distinct stages of disease progression possess identical gene expression signatures. In contrast, many other standard methods of analysis of gene expression signatures are dependent upon identification of overall differences between or among groups.

This study did not include subjects with an initial CIS that did not develop MS. The rationale for not including this parameter is three-fold. First, there is not a uniform clinical definition of CIS. Second, subjects with a CIS may or may not have MRI findings indicating inflammation or demyelination and the probability that a subject with CIS will develop MS is greater if MRI lesions are also detected. Third, with the current knowledge, it is uncertain if it is experimentally possible to absolutely conclude that a person with CIS will not develop MS. In fact, the period of time between an initial CIS and diagnosis of clinically definite MS is quite variable and can exceed 5 years.

Methods

Patients.

Blood samples in PAXgene tubes were obtained from CTRL, MS, OND-I and OND-NI subjects. Demographic characteristics of these cohorts have been previously described. Age, race and gender were not statistically different among the different study groups. Time of blood draw, for example, morning/afternoon clinics, was also not statistically significant among the different study groups. Relevant institutional review board approval was obtained from all participating sites.

Samples were also obtained from subjects with a clinically isolated syndrome (CIS) at the time of the blood draw. All of these subjects have gone on to develop MS according to the McDonald's criteria for the diagnosis of MS.

Transcript Determinations.

Total RNA purification, cDNA synthesis, and analysis using the 384-well Taqman Low Density Array (TLDA) were as previously described (FIG. 8).⁴⁰ Patient diagnosis was blinded for all experimental procedures. Relative expression levels were determined directly from the observed threshold cycle (C_(T)). Linear expression levels were determined using the formula, 2^((40-CT)).

Ratioscore and Support Vector Machine Algorithms.

The identification of the gene expression ratios and permutation testing strategy employed to identify discriminatory combinations of ratios to create the ratioscore have been previously described.^(40 and Example 1) Briefly, all possible gene-expression ratios of the 35 genes were computed. Ratios in which the greatest number of subjects in case groups possessed a ratio value greater than the highest ratio value in the control group were saved. Permutation testing was performed by randomly selecting 80% of the control group to compare with the case group and repeating this process 200 times producing 200 subsets of ratios. From these subsets of ratios, the smallest number of ratios to identify the ratioscore with maximum separation between case groups and control groups were identified. For example, MS versus CTRL, MS versus OND, etc. were compared. Each comparison produced a unique set of ratios that were used to define the ratioscore algorithm for that pairing of the case-control groups.

A support vector machine (SVM) was created from each set of ratioscores using LS-SVMLab software (http://www.esat.kuleuven.be/sista/Issvmab). For example, the gene-expression ratios from the MS versus CTRL were used to create a SVM for this type of comparison. The SVM was trained with L-fold cross-validation using 60% of the data. In this type of training a certain fraction of the training set was omitted from training and the remaining portion of the partial training set was used to estimate the parameters in the SVM. Once the SVM was trained, the SVM was applied to the total data set. Numbers of correct and incorrect classifications were tabulated for total sets (training and validation), training sets and validation sets. As expected, the overall accuracy in the training sets was greater than overall accuracy of the validation sets.

SEQUENCES

The following are complementary DNA (cDNA) sequences of genes-of-interest identified in Table A. The portion of the sequences bolded and underlined are Applied BioSystems context sequences, the region of that can be amplified in some embodiments of the presently-disclosed subject matter. ABI assay numbers for the sequences are provided in Table A.

SEQ ID NO: 1 - Homo sapiens active BCR-related gene (ABR), transcript variant 3, mRNA GGACTGCAGAGGGAACTTGCCTTGAAGAGGCCTGGTCCTTAAAGAGACACAGCACACACGGCCCGACCGG CAGCCCCAGAGCAGAGGCTCCACTGATGGCAGGCGCCCCTGGCTAGGCTCTGAGGTTCCTTTGCCCTCGC CTTGCTGAATGGTGAGCCGCTGCCTCTCGGAGCCCGTCTCCTTGACAGCCTGCCCTCGGCTCCTGCAGCC ACTCCTGGGCCTGATGGGGACAGGGCCAGCCTGGTGGGTGGTGTCAGAGGTCCTGGCAGAGCAGCGTAGG CCTGGGATGCGTCTGCAGAATTCTGGCTGAACGAGCGAGGAGCACGGCCAGCTTCGGGGCCGTCGTGACC ACAGGAGGGCAGAGGGCCAGCCCGTGAGCTCTGACCCCAGCTGGACGTGCTCTTGTTTCCCTTGGGGCTA AGGAGATTGGAGCCACTGAACTGAATCTCTGGGTTTTGGAGACTTAGAGAATCCATTGGACTCTTCTGCT GGCGTCTTTCTGAATGCTGATGGGGACTTGGTGACTTCAGCTACGGGACGGACGAGTACGACGGAGAGGG GAATGAGGAGCAGAAGGGGCCCCCGGAGGGCTCAGAGACCATGCCGTACATCGATGAGTCGCCCACCATG TCCCCGCAGCTCAGCGCCCGCAGCCAGGGCGGGGGGGATGGCGTCTCCCCGACTCCACCTGAGGGACT GG CTCCTGGGGTGGAAGCAGGGAAA GGCCTGGAGATGAGGAAGCTGGTTCTCTCGGGGTTCTTGGCCAGCGA AGAGATCTACATTAACCAGCTGGAAGCCCTGTTGCTGCCCATGAAACCCCTGAAGGCCACCGCCACCACC TCCCAGCCCGTGCTCACCATCCAGCAGATCGAGACCATCTTCTACAAGATCCAGGACATCTATGAGATCC ACAAGGAGTTCTATGACAACCTGTGCCCCAAGGTGCAACAGTGGGACAGCCAGGTCACCATGGGCCACCT CTTCCAGAAGCTGGCCAGCCAGCTCGGTGTGTACAAAGCGTTTGTCGATAACTATAAAGTCGCTCTGGAG ACAGCTGAGAAGTGCAGCCAGTCCAACAACCAGTTCCAGAAGATCTCAGAGGAACTCAAAGTGAAAGGTC CCAAGGACTCCAAGGACAGCCACACGTCTGTCACCATGGAAGCTCTGCTCTACAAGCCCATTGACCGGGT CACTCGGAGCACCCTAGTCCTACACGACCTGCTGAAGCACACACCTGTGGACCACCCCGACTACCCGCTG CTGCAGGATGCCCTCCGCATCTCCCAGAACTTCCTGTCCAGCATCAACGAGGACATCGACCCCCGCCGGA CTGCAGTGACAACGCCCAAGGGGGAGACGCGACAGCTGGTGAAGGACGGCTTCCTGGTGGAAGTGTCAGA GAGCTCCCGGAAGCTGCGGCACGTCTTCCTCTTTACAGATGTCCTACTGTGTGCCAAGCTGAAGAAGACC TCTGCAGGGAAGCACCAGCAGTATGACTGTAAGTGGTACATCCCCCTGGCCGACCTGGTGTTTCCATCCC CCGAGGAGTCTGAGGCCAGCCCCCAGGTGCACCCCTTCCCAGACCATGAGCTGGAGGACATGAAGATGAA GATCTCTGCCCTCAAGAGTGAAATCCAGAAGGAGAAAGCCAACAAAGGCCAGAGCCGGGCCATCGAGCGC CTGAAGAAGAAGATGTTTGAGAATGAGTTCCTGCTGCTGCTCAACTCCCCCACAATCCCGTTCAGGATCC ACAATCGGAATGGAAAGAGTTACCTGTTCCTACTGTCCTCGGACTACGAGAGGTCAGAGTGGAGAGAAGC AATTCAGAAACTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCAGTGGAGCTCCAGGTGCTCACA GGATCCTGTTTCAAGCTTAGGACTGTACACAACATTCCTGTCACCAGCAATAAAGACGACGATGAGTCTC CAGGACTCTATGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGATTTAAGCAATCAGCCAACCTGTA CTGTACCCTGGAGGTGGATTCCTTCGGCTATTTTGTCAGCAAAGCCAAAACCAGGGTGTTCCGGGACACA GCGGAGCCCAAGTGGGATGAGGAGTTTGAGATCGAGCTGGAGGGCTCCCAGTCCCTGAGGATCCTGTGCT ATGAGAAGTGCTATGACAAGACCAAGGTCAACAAGGACAACAATGAGATCGTGGACAAGATCATGGGCAA AGGACAGATCCAGCTGGACCCACAAACCGTGGAGACCAAGAACTGGCACACGGACGTGATTGAGATGAAC GGGATCAAAGTGGAATTTTCCATGAAATTCACCAGCCGAGATATGAGCCTGAAGAGGACCCCGTCCAAAA AGCAGACCGGCGTCTTCGGTGTGAAGATCAGCGTGGTGACGAAGCGGGAGCGCTCCAAGGTGCCCTACAT CGTCCGGCAGTGTGTGGAGGAGGTGGAGAAGAGGGGTATCGAGGAGGTTGGCATCTACAGGATATCGGGC GTGGCCACGGACATCCAGGCGCTCAAGGCCGTCTTCGATGCCAATAACAAGGACATCCTGCTGATGCTGA GTGACATGGACATCAACGCCATCGCCGGGACGCTCAAGCTGTACTTCCGGGAACTGCCCGAGCCGCTCCT CACGGACCGACTCTACCCAGCCTTCATGGAGGGCATCGCCCTGTCAGACCCTGCTGCCAAGGAAAACTGC ATGATGCACCTGCTCCGCTCCCTGCCCGACCCCAACCTCATCACCTTCCTCTTCCTGCTGGAACACTTGA AAAGGGTTGCCGAGAAGGAGCCCATCAACAAAATGTCACTTCACAACCTGGCTACCGTGTTTGGACCCAC GTTACTGAGACCCTCAGAAGTGGAGAGCAAAGCACACCTCACCTCGGCTGCGGACATCTGGTCCCATGAC GTCATGGCGCAGGTCCAGGTCCTCCTCTACTACCTGCAGCACCCCCCCATTTCCTTCGCAGAACTCAAGC GGAACACACTGTACTTCTCCACCGACGTGTAGCCCGAGGCAGGGTGGCTGCGGGCGGGTGGTGGAACCAG CCCCTCCAGCCTGGGGTCCAACTCAGACTTGAAAGACTGCAATAGAAAACTCCCAAACCCAGCACTCCAG ACTCGAGGGAAGCCAGCTTCCAAGAACTGGAATGCGTACGTCTTTTGTGCCACCTTGTACAAAGCCGGCT GCCCAGCCCCAGCCTCACCACCGCATCCCACCTCCTGCCCTCCATACCTCTAGTTGTGTCTGATGCTCCG TGCTGTTCGGGAATTGTTTTATGTACACTTGTCAGGCAGAAAAGGTAGTGACCGGCCCGGCGTGGGCACA CAGACAGCCCGCTTTGTTCTTTCATTTCCTCCAGCACTTTCTTTCCGCCTGAGTCCAGCCCAAGGCCTTT TATTTTGCGCTGTGTAACTGCTGCCAGCTTCTCTCTTGGCCCTGCTCCCAGATGGCGGTCTCCTGGCAGC CTCCCCTCAGTCTTCCTCCACCCGCTCTTCCTTCCCAGCCTGCCTGCATGCATGTGCACCCTTGGTCTTC GCTCCATCGCCTTGAAAGCTCTGAAGAGGCCCTGGGTTGCCGCGGCAGCAGTGGTCTGTTTGATGCTGCC GTTTGCCGCTGCCGGCCCCTCCTCAGACTCCGCCTTTGGGAGCACACCTGCTTTGCCTTGCTGCCTGTGC AAATGTTGGACAAGCAGACACACTCACACTCGTCCCCAGCTTAGCACAGAGCTGGAGCGCCCATTTCTGG AATTTTCCGTTTGGGAATCTCCACTTCTGGGGTTTACCTGTTCGGCCTCCTGTCTATCAGTGAGGCATCT CTGACTGTTTCTTCTACTGCTTTTCAGTTCCCTTCCCTGCTGTTCTATTTCCTTTGAGTGTAAAGACTCA CAGGTGACCTGCTATCGAGATAGCCAGAGGGTCAGGAGAGAATGGGGGAGGAGGCGGTCAGGCTGCTGAG GAAACACCACAGGCTGAACGGGGGAGGAATGCACATGCCACGCTGGGTGTCCCGGGTCGCGGGGAGGCAG CTCAGCTCTTAGGAGCAAGTTGTGGGGGCTTTTCAAGAGGGGCCAGGCTTCCTGGAGGGTGACTGATGTG GCCGAAGCAGGTGTCCAGGCAGGTAGGCTGCAGCCAGGAGCTCCCTGGCACCGCAGGACCTCGTGGTACT CTTGCCTTAGATTTTACACACACTCCACAGCCAAGCACTGCCACGGTCCTCCAGGACCTGGGAAGCAAAG GCACAGGCCCACGGTGGCCAGCCATTGTGGTGCCGCCCCAGCTTCTGGATACAGCCTTTTGGGTAAACAC TGGGAACTCCAGAAGTTGTGGGGAGAGTGGGGAATCAGACAGCCGCCTCTAGGGGCTGGGTTCTGCTGGG GCCTCCTTGTTGGTGCTGTAGGCACCCGCCAGGGAGCAGGGACCCGACTTGCAGACGCATTGCCCGGTAC TAGGAAGGAGTGAGGTGTGTTCCCACCGTACACTTCCCACACGAGCTGCGGCTGCCAGCCTCGGGCCATC AGCCTAGGAGAGCAGATGCAGCTCCAGGGGCTCGACTTATAGCCAGTTACAGCTCCCCGGCTCTTCTGTG TGGCAGAGCGTCGTTTCCGGGCCCTCAGGGCTGGGGAGCTCAGTTCCCATTGCTTGTGCTCAGGGCTGAG TCTTAAAGAAGGGTTTGCCGGCCCTAACGCTGCAGCGCGTGCGCGGTGAGAGGCCCTTTTTGAGCCTGTT TACTCCTGTGGCCTTGGGCAGAACAGTAAATACTCTGTGCACGGAGGAAAGACATGCCCAAGAGGAAGGA AGTACTGACCATCGGCTGCCTGTGAGCAGCTTAGCAAGGAGCCCTTGCTCCCTGGGAAAGGCGGTGAACT TGAGTCTAAAGATGCAGTGCCTGGCCCTTCCTAAGGTCCCTGCCTGGCATCCGAGTGTCGGTGTGTGGCA CAGAAGGCTCCTGCTTGCTTCCAAAGTGATGGACAGGAAGGGGCAGAGTGAGTCACGGCCCAGACTGGGC ACCTTCGCGTCTCAGCCTCAGGGAGCCCCACAGCCCCAAGCTCGCTGAGGCAACGTGAGAACAGGCTATG GGAAGGCTGCAAAGGCTGAGAAATGCAAAGGCTCATATTTATAAATCCCACCCCCAGAGTGGGGAGGGTC AGGTGCCAGACCTGGACTAAACTGCACCAAGGAAACACCCAGCAGGGTCTCCTGTGAGCCGGGGACCATG CAGCCCGAAACCTCCAGTCACTGCGCCCGGCAGGAGTCAGGAGCCAGGGACTGTGCAGCCTGGAACCTCC AGTCACTGTGCCCAGCAGGGTGGGCTGTGCCCAGCAGGAGTCAGGCTAAGAAACGCCAGGTCTGCCTGTT CTTGCTGGGCAATGGCTGATGGCTGCCAGTTTCTGCTGATACACAGGTAGGATGGGACCCTTCATGAATA TCTGACTTTAATAAGTTGGTAAGGATATATTTTTTTGTCTATGTTCTGTTTCAACTTATGTAGATTATTA TAAATTGATGTAAACCACGTGAGAGGAAAATGTTAATAAAAAATGCAAAGCCCCATCATTTGCACAAAAC TCA SEQ ID NO: 2 - Homo sapiens actin, beta (ACTB), mRNA ACCGCCGAGACCGCGTCCGCCCCGCGAGCACAGAGCCTCG CCTTTGCCGATCCGCCGCCCGTCCA CACCC GCCGCCAGCTCACCATGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGC CGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGGC GTGATGGTGGGCATGGGTCAGAAGGATTCCTATGTGGGCGACGAGGCCCAGAGCAAGAGAGGCATCCTCA CCCTGAAGTACCCCATCGAGCACGGCATCGTCACCAACTGGGACGACATGGAGAAAATCTGGCACCACAC CTTCTACAATGAGCTGCGTGTGGCTCCCGAGGAGCACCCCGTGCTGCTGACCGAGGCCCCCCTGAACCCC AAGGCCAACCGCGAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCATGTACGTTGCTA TCCAGGCTGTGCTATCCCTGTACGCCTCTGGCCGTACCACTGGCATCGTGATGGACTCCGGTGACGGGGT CACCCACACTGTGCCCATCTACGAGGGGTATGCCCTCCCCCATGCCATCCTGCGTCTGGACCTGGCTGGC CGGGACCTGACTGACTACCTCATGAAGATCCTCACCGAGCGCGGCTACAGCTTCACCACCACGGCCGAGC GGGAAATCGTGCGTGACATTAAGGAGAAGCTGTGCTACGTCGCCCTGGACTTCGAGCAAGAGATGGCCAC GGCTGCTTCCAGCTCCTCCCTGGAGAAGAGCTACGAGCTGCCTGACGGCCAGGTCATCACCATTGGCAAT GAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCTGGGCATGGAGTCCTGTGGCATCCACG AAACTACCTTCAACTCCATCATGAAGTGTGACGTGGACATCCGCAAAGACCTGTACGCCAACACAGTGCT GTCTGGCGGCACCACCATGTACCCTGGCATTGCCGACAGGATGCAGAAGGAGATCACTGCCCTGGCACCC AGCACAATGAAGATCAAGATCATTGCTCCTCCTGAGCGCAAGTACTCCGTGTGGATCGGCGGCTCCATCC TGGCCTCGCTGTCCACCTTCCAGCAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCCCCTCCAT CGTCCACCGCAAATGCTTCTAGGCGGACTATGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAAC TTGCGCAGAAAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTGTTTTGTTTTGGTTTTTTTTTT TTTTTTGGCTTGACTCAGGATTTAAAAACTGGAACGGTGAAGGTGACAGCAGTCGGTTGGAGCGAGCATC CCCCAAAGTTCACAATGTGGCCGAGGACTTTGATTGCACATTGTTGTTTTTTTAATAGTCATTCCAAATA TGAGATGCGTTCTTACAGGAAGTCCCTTCCCATCCTAAAAGCCACCCCACTTCTCTCTAAGGAGAATGGC CCAGTCCTCTCCCAAGTCCACACAGGGGAGGTGATAGCATTGCTTTCGTGTAAATTATGTAATGCAAAAT TTTTTTAATCTTCGCCTTAATACTTTTTTATTTTGTTTTATTTTGAATGATGAGCCTTCGTGCCCCCCCT TCCCCCTTTTTTGTCCCCCAACTTGAGATGTATGAAGGCTTTTGGTCTCCCTGGGAGTGGGTGGAGGCAG CCAGGGCTTACCTGTACACTGACTTGAGACCAGTTGAATAAAAGTGCACACCTTAAAAATGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 3 - Homo sapiens ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (ACTR1A), mRNA GCTCCCTCGCCGCCCTGAACCGGCGGCTAGACTGCGCATGCGTGTCAGTGGCGCTAGCGGCGGACCCGGC TGGGCAGTTCCTTCCCCAGAAGGAGAGATTCCTCTGCCATGGAGTCCTACGATGTGATCGCCAACCAGCC TGTCGTGATCGACAACGGATCCGGTGTGATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGC TTTCCAAACTATGTGGGCCGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCA TTGGCCCCAAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTCAA GGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAGACTTTCTCAGAG GAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAACCGGGAACGAGCTGCCGAAGTTT TCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCCATGCAAGCTGTACTCAGCCTTTACGCTACAGG CAGGACCACAGGGGTGGTGCTGGATTCTGGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTT GCCATGCCCCACTCCATCATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACC TGCGTAAGGAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCA AGGCCATAAAAGAAAGAGC CTGTTA CCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAGTACTACCTGCCT GATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAGTTGCTCTTCAGGCCAGATTTGA TTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTGTTCGCCATTCAGAAGTCAGACATGGACCTGCG GCGCACGCTTTTCTCTAACATTGTCCTCTCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTC CTGAGTGAAGTGAAGAAACTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGT ATTCCACGTGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAAAA GGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAATGTCGGGACATCATCTTCACC TCTCTCTGAAGTTAACTCCACTTTAAAACTCGCTTTCTTGAGTCGGAGTGTTTGCGAGGAACTGCCTGTG TGTGAGTGCGTGTGTGGATATGAGTGTGTGTGCACATGCGAGTGCCGTGTGGCCCTGGGACCCTGGGCCC AGAAAGGACGATGAACTACCTGCAGTGGTGATGGCCTGAGGCCTGGGGTTGACCACTAACTGGCTCCTGA CAGGGAAGAGCGCTGGCAGAGGCTGTGCTCCCTCCTCAGGTGGCCTCTGGCTGGCTGTGGGGGACTCCGT TTACTACCACAGGGAGACAGAGGGAGGTAAGCCATCCCCCGGGAGACCTTGCTGCTGACCATCCTAGGCT GGGCTGGCCCCACCCTCACCCCCACCCCCAGGGTGCCCTGAGGCCCCAGGCAGCTGCTGCCTCCACTATC GATGCCTCCTGACTGCACACTGAGGACTGGGACTGGGGTTGAGTTCTGTCTGGTTTTGTTGCCATTTTGG TTTGGGAGGCTGGAAAAGCACCCCAAGAGCTATTACAGAGACTGGAGTCAGGAGAGAGCAGGAGGCCCTC ATGTTCACCAGGGAACAGGACCACACCGGCCACTGGAGGAGGGCAGGAGCAGTCCTCACTCTGAATGGCT GCAGAGTTAATGTTCCCAGCCCAGTCCCCTTTCGGGGGCCTTGGGAGAGTTTAAGGCACCTGCTGGTTCC AGGACCTCGCTTTCCATCTGTTCTTGTTGCAATGCCATCTTCAAACCGTTTTATTTATTGAAGTGTTTGT TCAGTTAGGGGCTGGAGAGAGGGAGCTTGCTGCCTCCTGCCTTGCTACACTAATGTTTACAGCACCTAAG CTTAGCCTCCAGGGCCCCACCTCTCCCAGCTGATGGTGAGCTGACAGTGTCCACAGGTTCCAGGACCATT TGAGATTGGAAGCTACACTCAAAGACACTCCCACCAGGCTCTTTCTCCCTTTTCCTCTTGCTCACTGCCC TGGAATCAACAGGCTGGTTGCTGGTTAGATTTTCTGAAACAGGAGGTAAAATTTTTCTTTGGCAGAGGCC CCTAAGCAAGGGAGGGGTGTTGGAGAGCCAGTGCCCTTAAGACTGGAGAAAGCTGCAATTTACCAAGTTG CCTTTTGCCACTGTAGCTGACCAGGGGACTAGGTTGTAGAGGTGGGAAGGCCCCCTCTGGGCTGATCTTG TGCCATTCTTGACCTTGGACCTGCTTGGTTAAGGAGGGAGTGGGCCAGACCAGAGTGCCAGGAGCTAATG GAGCCAGGCCTGACTCCTAGGAGTGGTCCAAAGGCCTTCAGCCTAGATGGTGCAAAGCTGGGGCCAGCCT GTCTTCACCGGCACCCTCACCTGTGACACCAAGACCCACCCCAATCCCAGACTTCACACAGTATTCTCCC CCACGCCGTCCTATGACCAAAGGCCCCTGCCAGGTGTGGGCCACAGCAGCAGGTATGTGTGAAAGCAACG TAGCGCCCCGCGGACTGCAGTGCGCTTAACCAACTCACCTCCCTTCTCTTAGCCCAAGCCTGTCCCTCGC ACAGCCTCGCACAAACCACATTGCCTGGTGGGGCCCAGTGTACTGAAATAAAGTCGTTCCGATAGACACG TCAAAAAAAAAAAAAAAAAAA SEQ ID NO: 4 - Homo sapiens ADAMTS-like 4 (ADAMTSL4), transcript variant 1, mRNA CCGCCGCGGAGCGAGGTTGCCTGGAGAGAGCGCCTGGGCGCAGAAGGGTTAACGGGCCACCGGGGGCTCG CAGAGCAGGAGGGTGCTCTCGGACGGTGTGTCCCCCACTGCACTCCTGAACTTGGAGGACAGGGTCGCCG CGAGGGACGCAGAGAGCACCCTCCACGCCCAGATGCCTGCGTAGTTTTTGTGACCAGTCCGCTCCTGCCT CCCCCTGGGGCAGTAGAGGGGGAGCGATGGAGAACTGGACTGGCAGGCCCTGGCTGTATCTGCTGCTGCT TCTGTCCCTCCCTCAGCTCTGCTTGGATCAGGAGGTGTTGTCCGGACACTCTCTTCAGACACCTACAGAG GAGGGCCAGGGCCCCGAAGGTGTCTGGGGACCTTGGGTCCAGTGGGCCTCTTGCTCCCAGCCCTGCGGGG TGGGGGTGCAGCGCAGGAGCCGGACATGTCAGCTCCCTACAGTGCAGCTCCACCCGAGTCTGCCCCTCCC TCCCCGGCCCCCAAGACATCCAGAAGCCCTCCTCCCCCGGGGCCAGGGTCCCAGACCCCAGACTTCTCCA GAAACCCTCCCCTTGTACAGGACACAGTCTCGGGGAAGGGGTGGCCCACTTCGAGGTCCCGCTTCCCACC TAGGGAGAGAGGAGACCCAGGAGATTCGAGCGGCCAGGAGGTCCCGGCTTCGAGACCCCATCAAGCCAGG AATGTTCGGTTATGGGAGAGTGCCCTTTGCATTGCCACTGCACCGGAACCGCAGGCACCCTCGGAGCCCA CCCAGATCTGAGCTGTCCCTGATCTCTTCTAGAGGGGAAGAGGCTATTCCGTCCCCTACTCCAAGAGCAG AGCCATTCTCCGCAAACGGCAGCCCCCAAACTGAGCTCCCTCCCACAGAACTGTCTGTCCACACCCCATC CCCCCAAGCAGAACCTCTAAGCCCTGAAACTGCTCAGACAGAGGTGGCCCCCAGAACCAGGCCTGCCCCC CTACGGCATCACCCCAGAGCCCAGGCCTCTGGCACAGAGCCCCCCTCACCCACGCACTCCTTAGGAGAAG GTGGCTTCTTCCGTGCATCCCCTCAGCCACGAAGGCCAAGTTCCCAGGGTTGGGCCAGTCCCCAGGTAGC AGGGAGACGCCCTGATCCTTTTCCTTCGGTCCCTCGGGGCCGAGGCCAGCAGGGCCAAGGGCCTTGGGGA ACGGGGGGGACTCCTCACGGGCCCCGCCTGGAGCCTGACCCTCAGCACCCGGGCGCCTGGCTGCCCCTGC TGAGCAACGGCCCCCATGCCAGCTCCCTCTGGAGCCTCTTTGCTCCCAGTAGCCCTATTCCAAGATGTTC TGGGGAGAGTGAACAGCTAAGAGCCTGCAGCCAAGCGCCCTGCCCCCCTGAGCAGCCAGACCCCCGGGCC CTGCAGTGCGCAGCCTTTAACTCCCAGGAATTCATGGGCCAGCTGTATCAGTGGGAGCCCTTCACTGAAG TCCAGGGCTCCCAGCGCTGTGAACTGAACTGCCGGCCCCGTGGCTTCCGCTTCTATGTCCGTCACACTGA AAAGGTCCAGGATGGGACCCTGTGTCAGCCTGGAGCCCCTGACATCTGTGTGGCTGGACGCTGTCTGAGC CCCGGCTGTGATGGGATCCTTGGCTCTGGCAGGCGTCCTGATGGCTGTGGAGTCTGTGGGGGTGATGATT CTACCTGTCGCCTTGTTTCGGGGAACCTCACTGACCGAGGGGGCCCCCTGGGCTATCAGAAGATCTTGTG GATTCCAGCGGGAGCCTTGCGGCTCCAGATTGCCCAGCTCCGGCCTAGCTCCAACTACCTGGCACTTCGT GGCCCTGGGGGCCGGTCCATCATCAATGGGAACTGGGCTGTGGATCCCCCTGGGTCCTACAGGGCCGGCG GGACCGTCTTTCGATATAACCGTCCTCCCAGGGAGGAGGGCAAAGGGGAGAGTCTGTCGGCTGAAGGCCC CACCACCCAGCCTGTGGATGTCTATATGATCTTTCAGGAGGAAAACCCAGGCGTTTTTTATCAGTATGTC ATCTCTTCACCTCCTCCAATCCTTGAGAACCCCACCCCAGAGCCCCCTGTCCCCCAGCTTCAGCCGGAGA TTCTGAGGGTGGAGCCCCCACTTGCTCCGGCACCCCGCCCAGCCCGGACCCCAGGCACCCTCCAGCGTCA GGTGCGGATCCCCCAGATGCCCGCCCCGCCCCATCCCAGGACACCCCTGGGGTCTCCAGCTGCGTACTGG AAACGAGTGGGACACTCTGCATGCTCAGCGTCCTGCGGGAAAGGTGTCTGGCGCCCCATTTTCCTCTGCA TCTCCCGTGAGTCGGGAGAGGAACTGGATGAACGCAGCTGTGCCGCGGGTGCCAGGCCCCCAGCCTCCCC TGAACCCTGCCACGGCACCCCATGCCCCCCATACTGGGAGGCTGGCGAGTGGACATCCTGCAGCCGCTCC TGTGGCCCCGGCACCCAGCACCGCCAGCTGCAGTGCCGGCAGGAATTTGGGGGGGGTGGCTCCTCGGTGC CCCCGGAGCGCTGTGGACATCTCCCCCGGCCCAACATCACCCAGTCTTGCCAGCTGCGCCTCTGTGGCCA TTGGGAAGTTGGCTCTCCTTGGAGCCAGTGCTCCGTGCGGTGCGGCCGGGGCCAGAGAAGCCGGCAGGTT CGCTGTGTTGGGAACAATGGTGATGAAGTGAGCGAGCAGGAGTGTGCGTCAGGCCCCCCGCAGCCCCCCA GCAGAGAGGCCTGTGACATGGGGCCCTGTACTACTGCCTGGTTCCACAGCGACTGGAGCTCCAAGTGCTC AGCCGAGTGTGGGACGGGAATCCAGCGGCGCTCTGTGGTCTGCCTTGGGAGTGGGGCAGCCCTCGGGCCA GGCCAGGGGGAAGCAGGAGCAGGAACTGGGCAGAGCTGTCCAACAGGAAGCCGGCCCCCTGACATGCGCG CCTGCAGCCTGGGGCCCTGTGAGAGAACTTGGCGCTGGTACACAGGGCCCTGGGGTGAGTGCTCCTCCGA ATGTGGCTCTGGCACACAGCGTAGAGACATCATCTGTGTATCCAAACTGGGGACGGAGTTCAACGTGACT TCTCCGAGCAACTGTTCTCACCTCCCCAGGCCCCCTGCCCTGCAGCCCTGTCAAGGGCAGGCCTGCCAGG ACCGATGGTTTTCCACGC CCT GGAGCCCATGTTCTCGCTCCTG CCAAGGGGGAACGCAGACACGGGAGGT CCAGTGCCTGAGCACCAACCAGACCCTCAGCACCCGATGCCCTCCTCAACTGCGGCCCTCCAGGAAGCGC CCCTGTAACAGCCAACCCTGCAGCCAGCGCCCTGATGATCAATGCAAGGACAGCTCTCCACATTGCCCCC TGGTGGTACAGGCCCGGCTCTGCGTCTACCCCTACTACACAGCCACCTGTTGCCGCTCTTGCGCACATGT CCTGGAGCGGTCTCCCCAGGATCCCTCCTGAAAGGGGTCCGGGGCACCTTCACGGTTTTCTGTGCCACCA TCGGTCACCCATTGATCGGCCCACTCTGAACCCCCTGGCTCTCCAGCCTGTCCCAGTCTCAGCAGGGATG TCCTCCAGGTGACAGAGGGTGGCAAGGTGACTGACACAAAGTGACTTTCAGGGCTGTGGTCAGGCCCATG TGGTGGTGTGATGGGTGTGTGCACATATGCCTCAGGTGTGCTTTTGGGACTGCATGGATATGTGTGTGCT CAAACGTGTATCACTTTTCAAAAAGAGGTTACACAGACTGAGAAGGACAAGACCTGTTTCCTTGAGACTT TCCTAGGTGGAAAGGAAAGCAAGTCTGCAGTTCCTTGCTAATCTGAGCTACTTAGAGTGTGGTCTCCCCA CCAACTCCAGTTTTGTGCCCTAAGCCTCATTTCTCATGTTCAGACCTCACATCTTCTAAGCCGCCCTGTG TCTCTGACCCCTTCTCATTTGCCTAGTATCTCTGCCCCTGCCTCCCTAATTAGCTAGGGCTGGGGTCAGC CACTGCCAATCCTGCCTTACTCAGGAAGGCAGGAGGAAAGAGACTGCCTCTCCAGAGCAAGGCCCAGCTG GGCAGAGGGTGAAAAAGAGAAATGTGAGCATCCGCTCCCCCACCACCCCGCCCAGCCCCTAGCCCCACTC CCTGCCTCCTGAAATGGTTCCCACCCAGAACTAATTTATTTTTTATTAAAGATGGTCATGACAAATGAGA AAAAAAAAA SEQ ID NO: 5 - Homo sapiens anaphase promoting complex subunit 1 (ANAPC1), mRNA CGCGTCCATTTGAACGTCTCGCACGCCTTCCTGCCATTAGCACTCGAGCCCGCTGCTGTTGCCCGTTCTT CCTCCAGAATAGGGGAGGGAGAGGGAATGAGAAGCTGCTGCGGCCCAAGAGTCACTGTGAAGGACCCCGC CGCTGCCCTCGGGCCTCCTCGGCCCCTGCGCCTCCGGGGAGCAGCCGGGGCTCGCCGCGCCTGACGCGTC CCGAGTTATACAGAAATAATGTTGATATTTGGAACCCATGTCGAACTTCTATGAAGAAAGGACAACGATG ATTGCAGCAAGGGATTTGCAGGAATTTGTTCCTTTTGGTCGAGACCACTGCAAGCACCACCCTAATGCTT TGAACCTTCAACTTCGCCAGCTGCAGCCAGCTTCTGAATTATGGTCTTCTGATGGTGCTGCTGGCTTGGT GGGATCCCTTCAGGAGGTTACAATCCACGAGAAACAGAAGGAAAGCTGGCAGTTAAGGAAAGGAGTAAGT GAAATTGGAGAAGATGTGGACTATGATGAGGAACTCTATGTTGCTGGAAATATGGTGATATGGAGCAAAG GAAGTAAAAGCCAGGCATTGGCAGTTTATAAAGCATTTACAGTTGACAGTCCTGTTCAGCAGGCATTGTG GTGTGACTTCATTATATCACAGGATAAGTCTGAAAAGGCCTACAGTAGCAATGAAGTAGAAAAATGCATA TGTATATTGCAAAGCTCATGTATTAACATGCATAGCATAGAAGGAAAGGATTACATAGCTTCATTACCAT TTCAGGTTGCAAATGTTTGGCCCACTAAATATGGATTGCTGTTTGAACGAAGCGCTTCTTCACATGAAGT ACCTCCAGGTTCACCCAGAGAACCTTTACCTACTATGTTCAGCATGCTGCACCCACTAGATGAAATAACT CCACTTGTTTGTAAATCTGGAAGTCTTTTTGGTTCATCACGGGTGCAATATGTTGTAGATCATGCAATGA AAATTGTTTTCCTCAATACTGACCCCTCTATTGTAATGACTTATGATGCTGTTCAAAATGTGCATTCTGT GTGGACTCTCCGGAGAGTCAAATCAGAGGAAGAGAATGTTGTTTTAAAGTTCTCTGAACAGGGGGGAACC CCACAGAATGTGGCCACTAGCAGCTCCCTCACAGCACATCTCAGAAGCCTCTCCAAAGGAGATTCCCCTG TGACTTCACCTTTCCAGAATTACTCCTCCATTCACAGCCAGAGTCGCTCAACCTCATCACCCAGTCTACA TTCTCGCTCACCTTCTATTTCCAACATGGCAGCTCTAAGTCGTGCTCATTCTCCTGCGTTAGGAGTGCAC TCTTTTTCAGGGGTGCAAAGGTTCAACATTTCAAGCCATAATCAGTCTCCAAAGAGACATAGTATTTCTC ATTCTCCAAATAGTAATTCTAATGGCTCCTTTCTTGCACCAGAAACGGAGCCAATTGTTCCTGAACTGTG TATTGACCATTTGTGGACAGAAACGATTACTAATATAAGAGAGAAAAATTCACAAGCCTCAAAAGTGTTT ATTACATCTGACCTATGTGGGCAAAAGTTCCTGTGCTTTTTAGTAGAGTCCCAGCTCCAGTTACGCTGTG TAAAGTTTCAAGAGAGTAATGATAAAACCCAGCTCATCTTTGGTTCAGTGACCAACATACCAGCAAAGGA TGCAGCACCAGTGGAGAAAATAGACACCATGCTGGTCTTGGAAGGCAGTGGAAACCTGGTGCTATACACA GGAGTGGTTCGGGTGGGAAAGGTTTTTATTCCTGGACTGCCAGCTCCCTCTCTGACGATGTCCAACACAA TGCCTCGGCCCAGTACTCCACTAGATGGCGTTAGTACTCCAAAGCCTCTTAGTAAACTCCTTGGATCATT GGACGAGGTTGTTCTGTTGTCCCCAGTTCCAGAACTGAGGGATTCTTCAAAACTTCATGATTCTCTCTAT AATGAGGATTGTACTTTCCAACAGCTTGGAACTTACATTCATTCTATCAGAGATCCTGTCCATAACAGAG TCACCCTGGAACTGAGTAATGGCTCCATGGTTAGGATCACTATTCCTGAAATTGCCACCTCTGAGTTAGT ACAAACGTGTTTGCAAGCAATTAAGTTTATCCTGCCAAAAGAAATAGCAGTTCAGATGCTTGTCAAGTGG TACAATGTCCACAGTGCTCCAGGAGGACCCAGTTATCACTCAGAGTGGAATTTATTTGTGACTTGTCTCA TGAACATGATGGGTTATAACACAGACCGCTTAGCATGGACTAGAAATTTTGACTTTGAAGGATCACTTTC TCCTGTCATTGCGCCCAAAAAAGCAAGGCCTTCCGAGACTGGATCTGATGATGACTGGGAATATTTACTA AATTCAGACTACCACCAGAATGTTGAGTCTCATCTTTTGAACAGATCTTTATGTCTGAGTCCTTCAGAAG CTTCACAGATGAAGGATGAGGATTTTTCACAGAATCTCAGTCTGGATTCTTCTACACTTCTCTTTACTCA CATACCTGCAATTTTTTTCGTTCTTCACCTTGTGTATGAGGAGCTTAAGTTGAATACTCTAATGGGAGAA GGAATTTGTTCACTTGTTGAACTTCTCGTTCAGTTGGCAAGGGACTTAAAATTGGGGCCTTATGTAGATC ATTACTATAGAGACTACCCAACGCTTGTCAGAACTACTGGACAAGTGTGCACAATTGATCCAGGTCAAAC AGGATTTATGCATCATCCATCATTTTTTACGTCTGAGCCACCAAGTATTTATCAGTGGGTGAGTTCTTGT CTGAAGGGTGAAGGAATGCCACCTTATCCTTACCTCCCTGGAATCTGTGAAAGAAGCAGACTTGTAGTCT TGAGTATTGCACTGTACATACTTGGTGATGAGAGCTTGGTTTCTGATGAATCCTCACAGTATTTAACCAG AATAACTATAGCCCCCCAGAAGTTGCAAGTAGAACAAGAGGAAAACAGGTTTAGTTTCAGGCATTCTACA TCTGTTTCTAGTCTAGCTGAAAGATTGGTTGTCTGGATGACTAATGTAGGATTCACTTTAAGAGATTTGG AAACTCTTCCCTTTGGAATTGCTCTTCCCATCAGAGATGCAATTTATCACTGTCGTGAGCAGCCTGCCTC AGACTGGCCAGAAGCTGTCTGTCTCTTGATTGGACGTCAGGATCTTTCCAAGCAGGCCTGCGAAGGAAAC TTACCCAAAGGGAAGTCTGTGCTCTCATCAGATGTTCCTTCAGGAACAGAAACTGAGGAGGAAGATGACG GCATGAATGACATGAATCACGAGGTCATGTCATTAATATGGAGTGAAGATTTAAGGGTGCAGGATGTGCG AAGGCTTCTTCAGAGTGCGCATCCTGTCCGTGTCAACGTAGTGCAGTACCCAGAGCTCAGTGACCACGAG TTCATCGAGGAAAAGGAAAACAGATTGCTCCAATTGTGTCAGCGAACTATGGCTCTTCCTGTAGGACGAG GAATGTTTACCTTGTTTTCGTACCATCCTGTTCCAACAGAGCCATTGCCTATTCCTAAATTGAATCTGAC TGGGCGTGCCCCTCCTCGGAACACAACAGTAGACCTTAATAGTGGAAACATCGATGTGCCTCCCAACATG ACAAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCTGGCCTGAAGATAGCTCCTGCCTCCCAGATCGACT CAGCTTGGATTGTTTACAATAAGCCCAAGCATGCTGAGTTGGCCAATGAGTATGCTGGCTTTCTCATGGC TCTGGGTTTGAATGGGCACCTTACCAAGCTGGCGACTCTCAATATCCATGACTACTTGACCAAGGGCCAT GAAATGACAAGCATTGGACTGCTACTTGGTGTTTCTGCTGCAAAACTAGGCACCATGGATATGTCTATTA CTCGGCTTCTTAGCATTCACATTCCTGCTCTCTTACCCCCAACGTCCACAGAGCTGGATGTTCCTCACAA TGTCCAAGTGGCTGCAGTGGTTGGCATTGGCCTTGTATATCAAGGGACAGCTCACAGACATACTGCAGAA GTCCTGTTGGCTGAGATAGGACGGCCTCCTGGTCCTGAAATGGAATACTGCACTGACAGAGAGTCATACT CCTTAGCTGCTGGCTTGGCCCTGGGCATGGTCTGCTTGGGGCATGGCAGCAATTTGATAGGTATGTCTGA TCTCAATGTGCCTGAGCAGCTCTATCAGTACATGGTTGGAGGACATAGGCGCTTTCAAACAGGAATGCAT AGGGAGAAACATAAATCACCAAGTTATCAAATCAAAGAAGGAGATACCATAAATGTGGATGTGACTTGTC CAGGTGCTACTCTAGCTTTGGCTATGATCTACTTAAAAACCAATAACAGATCTATTGCAGATTGGCTCCG AGCCCCTGACACCATGTATTTGTTGGACTTTGTGAAGCCAGAATTTCTCTTGCTTAGGACACTTGCTCGA TGCCTGATTTTGTGGGATGATATTTTACCAAATTCCAAGTGGGTTGACAGCAATGTTCCTCAAATTATAA GAGAAAATAGTATCTCTCTCAGTGAAATCGAATTGCCGTGCTCAGAGGATTTGAATTTGGAAACTTTGTC CCAAGCACATGTCTACATAATTGCAGGAGCCTGCTTGTCTCTGGGTTTTCGATTTGCTGGCTCAGAAAAC TTATCAGCATTTAACTGTTTGCATAAATTTGCCAAAGATTTTATGACTTATTTGTCCGCACCTAATGCTT CTGTTACAGGTCCTCATAACCTAGAAACTTGTCTGAGCGTGGTGCTGCTGTCTCTCGCCATGGTCATGGC TGGCTCAGGAAACCTAAAGGTTTTGCAGCTTTGTCGCTTCTTACACATGAAAACGGGTGGTGAAATGAAC TATGGTTTTCACTTAGCCCACCACATGGCCCTTGGACTTCTATTTTTGGGAGGAGGAAGGTACTCTTTGA GCACATCAAATTCTTCCATTGCCGCTCTTCTCTGTGCCCTTTATCCGCACTTCCCAGCTCACAGCACTG A CAA CCGGTATCATCTCCAGGCTCT CCGGCACCTCTATGTGCTGGCCGCGGAGCCCAGGCTTCTAGTGCCT GTGGATGTGGACACAAACACGCCCTGCTATGCCCTCTTAGAAGTTACCTACAAGGGCACTCAGTGGTATG AACAAACCAAAGAAGAATTGATGGCTCCTACCCTTCTTCCAGAACTCCATCTTTTAAAGCAGATTAAAGT AAAAGGCCCAAGATACTGGGAACTGCTCATAGATTTAAGCAAAGGAACACAACACTTGAAGTCCATCCTT TCCAAGGATGGGGTTTTATATGTTAAACTCCGGGCGGGTCAGCTCTCCTACAAAGAAGATCCAATGGGAT GGCAAAGTTTGTTGGCTCAGACTGTTGCTAACAGGAACTCTGAAGCCCGGGCTTTCAAGCCAGAAACAAT CTCAGCATTCACTTCTGATCCAGCACTTCTGTCATTTGCTGAATATTTCTGCAAGCCAACTGTGAACATG GGTCAGAAACAGGAAATTCTGGATCTCTTTTCTTCAGTACTCTATGAATGTGTTACCCAGGAGACCCCAG AGATGTTGCCTGCATACATAGCAATGGATCAGGCTATAAGAAGACTTGGGAGAAGAGAAATGTCTGAGAC TTCTGAACTTTGGCAGATAAAGTTGGTGTTAGAGTTTTTCAGCTCCCGAAGCCATCAGGAGCGGCTGCAG AACCACCCTAAGCGGGGGCTCTTTATGAACTCGGAATTCCTCCCTGTTGTGAAGTGCACCATTGATAATA CCCTGGACCAGTGGCTACAAGTCGGGGGTGATATGTGTGTGCACGCCTACCTCAGCGGGCAGCCCTTGGA GGAATCACAGCTGAGCATGCTGGCCTGCTTCCTCGTCTACCACTCTGTGCCAGCTCCACAGCACCTGCCA CCTATAGGACTAGAAGGGAGCACAAGCTTTGCTGAACTGCTCTTCAAATTTAAGCAGCTAAAAATGCCAG TGCGAGCTTTGCTGAGATTGGCTCCTTTGCTTCTTGGAAATCCACAGCCAATGGTGATGTGACCGTGTCT GGCGGTGAACCTACCCTGAAACGTGACTTCTGCACAACAAACGTGACCAAACATCAAAGCTAAAGCAATG TTTATAAAGTTTTATGGTATAACTAGGGGGAAATGAGCTGCACAAACCTCAATGTATTTTAAATCTGTTG CTGTCATCATTAACGGTATATGACATATAAAAGCAAGTTAAAATTTACTTTTGTAAATAAAGTTTTTGGT TTGTTTCCAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 6 - Homo sapiens apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F (APOBEC3F), transcript variant 1, mRNA TTCCCTTTGCAATTGCCTTGGGTCCTGCCGCACAGAGCGGCCTGTCTTTATCAGAGGTCCCTCTGCCAGG GGGAGGGCCCCAGAGAAAACCAGAAAGAGGGTGAGAGACTGAGGAAGATAAAGCGTCCCAGGGCCTCCTA CACCAGCGCCTGAGCAGGAAGGGGGAGGGGCCATGACTACGAGGCCCTGGGAGGTCACTTTAGGGAGGGC TGTCCTGAAACCTGGAGCCTGGAGCAGAAAGTGAAACCCTGGTGCTCCAGACAAAGATCTTAGTCGGGAC TAGCCGGCCAAGGATGAAGCCTCACTTCAGAAACACAGTGGAGCGAATGTATCGAGACACATTCTCCTAC AACTTTTATAATAGACCCATCCTTTCTCGTCGGAATACCGTCTGGCTGTGCTACGAAGTGAAAACAAAGG GTCCCTCAAGGCCCCGTTTGGACGCAAAGATCTTTCGAGGCCAGGTGTATTCCCAGCCTGAGCACCACGC AGAAATGTGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGTTTCCAGATCACCTGG TTTGTATCCTGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCCGAATTCCTGGCTGAGCACCCCAATG TCACCCTGACCATCTCCGCCGCCCGCCTCTACTACTACTGGGAAAGAGATTACCGAAGGGCGCTCTGCAG GCTGAGTCAGGCAGGGGCCCGCGTGAAGATTATGGACGATGAAGAATTTGCATACTGCTGGGAAAACTTT GTGTACAGTGAAGGTCAGCCATTCATGCCTTGGTACAAATTCGATGACAATTATGCATTCCTGCACCGCA CGCTAAAG GAG ATTCTCAGAAACCCGATGGAGG CAATGTATCCACACATATTCTACTTCCACTTTAAAAA CCTACGCAAAGCCTATGGTCGGAACGAAAGCTGGCTGTGCTTCACCATGGAAGTTGTAAAGCACCACTCA CCTGTCTCCTGGAAGAGGGGCGTCTTCCGAAACCAGGTGGATCCTGAGACCCATTGTCATGCAGAAAGGT GCTTCCTCTCTTGGTTCTGTGACGACATACTGTCTCCTAACACAAACTACGAGGTCACCTGGTACACATC TTGGAGCCCTTGCCCAGAGTGTGCAGGGGAGGTGGCCGAGTTCCTGGCCAGGCACAGCAACGTGAATCTC ACCATCTTCACCGCCCGCCTCTACTACTTCTGGGATACAGATTACCAGGAGGGGCTCCGCAGCCTGAGTC AGGAAGGGGCCTCCGTGGAGATCATGGGCTACAAAGATTTTAAATATTGTTGGGAAAACTTTGTGTACAA TGATGATGAGCCATTCAAGCCTTGGAAAGGACTAAAATACAACTTTCTATTCCTGGACAGCAAGCTGCAG GAGATTCTCGAGTGAGGGGTCTCCCCGGGCCTCATGGTCTGTCTCCTCTAGCCTCCTGCTCATGTTGTGC AGGCCTCCCCTCCATCCTGGACCAGCTGTGCTTTTGCCTGGTCATCCTGAGCCCCTCCTGGCCTCAGGGC CATTCCATAGTGCTCCCCTGCCTCACCACCTCCTCTCCGCTCTCCCAGGCTCTTCCTGCAGAGGCCTCTT TCTGCCTCCATGGCTATCCATCCACCCACCAAGACCCTGTTCCCTGAGCCTGCATGCCCCTAACCTGCCT TTTCCCATCTCCCCAGCATAACCTAATATTTTTTTTTTTTTTTTGAGACGGAATTTCGCTCTGTCACCCA GACTGGAGTGCAATGGCTTGATCTTGGCTCACTGCAAACTCTGCCTACCAGGTTCAAGCGATTCTCCTGC CTCCGCCTCCCGAGTAGCTGGAATTACAGACGCCTGCCACCACGCACAGCTAACTTTTTTTTTTTTTGTA TTTTTAGTAGTGACTGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCGC CTATCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGGCCCGGCGGCACAACCAAATCTTA TTAAACTCACCCTAGGCTGGCCGCGGTGACTCATGCCTATAATCCCCCAGCAATTTGGGAGGCAGAGGTG AGAGAATCGCTTGAGCCCAGGAATTCGAGACCAGCCTGGGCCACATGACAAAGCCCCATCTCTACAAAAA AATTACAAAAAAAAAAAAAACAGGTGTGGTGGCATGCACCTGTAGTTGAAGCTACTTGGAAGGATGAAGT GGGAGGATTGCTTGAGCCGGGGAGGTGGAGGCTGCAGTGAACTGAGATCACGTCACTGAACTCCAGTCTG AGCAACAGATCGAGACCCTGCCTGAAAATAAATCAATAAATAAACTCAACCGAAATGGGTATGAAAGTTG AAATGGGTATGTAAGTTGAAAACCAGAAGTTTTGAGAAACATCCTTTGTTAACTTTCATCCTACAAATTG GGTCATTCATGTCCTACGCAGCTAAAACAGAGCCCAGGAGCCAGGGAGGAAAAGCAGTCAGGCCACACAC CATTGCTCCCAAAATGGACTTCTCTGCAAGCCTGACTCCTGAAACTGTGCATTGTACCCTGAAACCAGCT TTATCCATAGCTTCTGCAATAAATGGCTGTAAGTCTTGGACTCCTTGCTATAATCGCAGCTATTCAGCAA TGGAACCTCCCAGTTCCCAACCCTTCCTAGTGCCCATGGGCTTTCCCATAGGACAAGAGAACATTTCTCC TTTTCTTTTTTTTTTTCTTTGAAATGGAGTCTCGCCCTGTCACCCAGGCTGGAGTGCAATGGTGCGGTCT CGGCTCACTGCAACCTCTGCCTCCCTTGTTCAAGTGATTCTCCTGTCTCAGCCTCCCGAGTAGCTGGGAT TACAGGCGTCCACCACCAAACCAGGCTAATTTTTGTATTTTTCATAAAAACGGGTTTCATCATGTTTCCC AGGCTGGTCTTATTTTTATTTTATTTTTTGAGATGGAGTCTTGCTCTGTTGCCCAGGCTGGGGTGCAGTG GTGCAATCTGGGTTCACTGCAGCCTCTGCCGCCTGAGTTCAAGCTATTTTCCTACCTAAGCCTCCCAAGT AGCTGGGATTACATGCGCGTGCCACCACGCCTAGCTAATTTTTGTGTTTTTAGTAGAGACGGGGTTTCAA CATCTTGACCAGGCTGGTCTTGAACTCCTGACCTCGTGATCCACCCGTCTCGGCCTCCCAAAGTGCTGGG ATTACAGGCGTGAGCCACCTGGCCAGGCTTAGGCTGGTCTTAAACTCCTGACCTCAAGTGATCCAACCTC CTTGGCCTCCCAAATTGCTGGGATTGCTGGTGTGAGCCACAGCGCCTAGCCCATTTCTCCTTTTAATAGG ACCTGTTGCTGTCTCTGTTCTCCCAACATGGTGAACACCACCCGGACTGCGTGTATGTCCCAAATTACAA TTCTTTCTTTGCAAATGAAATGTGAAATTTAGAGGCCCTTCTCCACACTTTAAATTTGACTTGACATTTT CTAGGCAGATATAAGTTATTAGAGAATGAGATTCTCTATAAAAATGATCCCTTCATGCTGTGGCCTCCAC AGAAGATGCCCTGGGCCAGGTGCCCACATGAATAATGCGGGCCACAGGCAGGCATTTATTTTCTCACAGA TATGGAGGCTACAAGTCCAAGGTGGAGGGGTCGGCGGGGTTGTTTGCTCTGAGGCCGCTCCTCCTGGATG GCAGGGATCCCTTCTGGCTGTGTCCTCTGTGGCCTTTCCTCTATGAACCTGTACTGTACCTCTGGGGTCT CTCTGCTTCCAAATATCTTTTTTTTTTTTTTCAGACAGTTTTGCTCTTGTTTTCTAGGCTGGAGTGCAAT GGCACAATCTCAGCTCACTGCAACCTCTGCCTTCCGAGTTCAAGCGATTCTCGTGCCTCAGCCTCCTGAG TAGCTGGGACTACAGGCGTGTGCCACCACGCCTGGCTAATTTTGTAGTTTTAGTAGAGACGGGGTTTCTC CATGTTGCTCAGGCTGGTCTTGAACTCATGAGCTCAGGCGATCCACTCTCCTCAGCCTCCCAAAGTGCTG GGATTACAGATATAAGCCACCATACACAACTTTTTTTTTTTTTTGAGATGGAGTTTCACTCTGTTGCCCA GGCTGGAGTGCTAAATAGCAGAATCACTGCTCACTGCAACCTCTGCCTGCTGGGTTCAAGCAATTCTCCC ACCTCAGCCTCCTGAGTAGCTGGGATTACAGATGCCCAGAACCAATCTCTGCTAATTTTTCTATTTTTTA GTAGAGATGGGGTTTCACTGAGGAAGGAGACCACCTCTCTCATTGTCTCCTATTTCAGAAGGAAGCAAAA AGTTAGAAAGATGCAGAAGTAAGATCAATGGCCAGACTGTTTGGCGCTGCTACCTGGGCCTGGTAGTTAA AGATCAACTCCTGACCTGACCGCTTGTTTTATCTAAAGATTCCAGACATTGTATGAGGAAGCATTGTGAA ACTTTCTGGTCTGTTCTGCTAGCCCCCACCACTGATGCATGTAGCCCCCCAGTCACGTAGCCCACGCTTG CACAATCTATCACGACCCTTTCACGTGGACCCCTTAGAATTGTAAGCCCTTAAAAGGGCCAGGGACTTCT TCAGGGAGCTCCAATCTTCAGATGCAAGTCTGTCAACGCTCCCAGCTGATTAAAGCCTCTTCCTTCCTAA AAAAAAAAAAAAAAAA SEQ ID NO: 7 - Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA CCAGGCGGAGGTGAGTGCGCGGCGGCCGGATGGGCGGGACGGGCGTGGAGGACGCCGAGCACCGTGGCGC GCGCTCACGTCCGCGTCCCCAAGGGCTGCGCTCCCTCAAGCGCAGTGCCCAGAACTCGGAGCCAGCCCGG CCCGGGGGACCCTGCTGGCCAAGGAGGTCGTCAGTCCGGTCTTGTCTTCCAGACCCGGAGGACCGAAGCT TCCGGACGACGAGGAACCGCCCAACATGGCCTCGGAGAGTGGGAAGCTTTGGGGTGGCCGGTTTGTGGGT GCAGTGGACCCCATCATGGAGAAGTTCAACGCGTCCATTGCCTACGACCGGCACCTTTGGGAGGTGGATG TTCAAGGCAGCAAAGCCTACAGCAGGGGCCTGGAGAAGGCAGGGCTCCTCACCAAGGCCGAGATGGACCA GATACTCCATGGCCTAGACAAGGTGGCTGAGGAGTGGGCCCAGGGCACCTTCAAACTGAACTCCAATGAT GAGGACATCCACACAGCCAATGAGCGCCGCCTGAAGGAGCTCATTGGTGCAACGGCAGGGAAGCTGCACA CGGGACGGAGCCGGAATGACCAGGTGGTCACAGACCTCAGGCTGTGGATGCGGCAGACCTGCTCCACGCT CTCGGGCCTCCTCTGGGAGCTCATTAGGACCATGGTGGATCGG GCAGAGGCGGAACGTGATGTTCTCT TC CCGGGGTACACCCATTTGCAGAGGGCCCAGCCCATCCGCTGGAGCCACTGGATTCTGAGCCACGCCGTGG CACTGACCCGAGACTCTGAGCGGCTGCTGGAGGTGCGGAAGCGGATCAATGTCCTGCCCCTGGGGAGTGG GGCCATTGCAGGCAATCCCCTGGGTGTGGACCGAGAGCTGCTCCGAGCAGAACTCAACTTTGGGGCCATC ACTCTCAACAGCATGGATGCCACTAGTGAGCGGGACTTTGTGGCCGAGTTCCTGTTCTGGGCTTCGCTGT GCATGACCCATCTCAGCAGGATGGCCGAGGACCTCATCCTCTACTGCACCAAGGAATTCAGCTTCGTGCA GCTCTCAGATGCCTACAGCACGGGAAGCAGCCTGATGCCCCAGAAGAAAAACCCCGACAGTTTGGAGCTG ATCCGGAGCAAGGCTGGGCGTGTGTTTGGGCGGTGTGCCGGGCTCCTGATGACCCTCAAGGGACTTCCCA GCACCTACAACAAAGACTTACAGGAGGACAAGGAAGCTGTGTTTGAAGTGTCAGACACTATGAGTGCCGT GCTCCAGGTGGCCACTGGCGTCATCTCTACGCTGCAGATTCACCAAGAGAACATGGGACAGGCTCTCAGC CCCGACATGCTGGCCACTGACCTTGCCTATTACCTGGTCCGCAAAGGGATGCCATTCCGCCAGGCCCACG AGGCCTCCGGGAAAGCTGTGTTCATGGCCGAGACCAAGGGGGTCGCCCTCAACCAGCTGTCACTGCAGGA GCTGCAGACCATCAGCCCCCTGTTCTCGGGCGACGTGATCTGCGTGTGGGACTACGGGCACAGTGTGGAG CAGTATGGTGCCCTGGGCGGCACTGCGCGCTCCAGCGTCGACTGGCAGATCCGCCAGGTGCGGGCGCTAC TGCAGGCACAGCAGGCCTAGGTCCTCCCACACCTGCCCCCTAATAAAGTGGGCGCGAGAGGAGGCTGCTG TGTGTTTCCTGCCCCAGCCTGGCTCCCTCGTTGCTGGGCTTTCGGGGCTGGCCAGTGGGGACAGTCAGGG ACTGGAGAGGCAGGGCAGGGTGGCCTGTAATCCCAGCACTTTGGAAGGGCAAGGTGCGAGGATGCTTGAG GCCAGGAGTTTGACACAGCCTGGGCAACACAGGGAGACCCCCATCTCTACTCAATAATAAAACAAATAGC CTGGCGTGGTGGCCCATGCATATAGTCCCAGCTACTTGTAAGGCTGAGGTGAGAGGACACTTGTGCCCAG GAGTGGAGGCTGCAGTGAGCTATGATCACGCCACTGCATTCCAGCCTGGATAACAGAGTGAGAACCTATC TCTAAAAATAAATAAATAAACGAAAAATAAA SEQ ID NO: 8 - Homo sapiens beta-2-microglobulin (B2M), mRNA AATATAAGTGGAGGCGTCGCGCTGGCGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCT CCGTGGCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCTGGCCTGGAGGCTATCCAGCGTACTCCAAAGAT TCAGGTTTACTCACGTCATCCAGCAGAGAATGGAAAGTCAAATTTCCTGAATTGCTATGTGTCTGGGTTT CATCCATCCGACATTGAAGTTGACTTACTGAAGAATGGAGAGAGAATTGAAAAAGTGGAGCATTCAGACT TGTCTTTCAGCAAGGACTGGTCTTTCTATCTCTTGTACTACACTGAATTCACCCCCACTGAAAAAGATGA GTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATA GTT AAGTGGGATCGAGACATGTAA G CAGCATCATGGAGGTTTGAAGATGCCGCATTTGGATTGGATGAATTCCAAATTCTGCTTGCTTGCTTTT TAATATTGATATGCTTATACACTTACACTTTATGCACAAAATGTAGGGTTATAATAATGTTAACATGGAC ATGATCTTCTTTATAATTCTACTTTGAGTGCTGTCTCCATGTTTGATGTATCTGAGCAGGTTGCTCCACA GGTAGCTCTAGGAGGGCTGGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTATCCAACATCAACATCTT GGTCAGATTTGAACTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATAAGTTAAC TTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGAAA TTTGTTATAATGAATGAAACATTTTGTCATATAAGATTCATATTTACTTCTTATACATTTGATAAAGTAA GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAACTTGATGTGTTA TCTCTTA SEQ ID NO: 9 - Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 6, non-coding RNA AGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCTGCTCTGGGTAAAGGTAGTAGAGTCCCGGGA AAGGGACAGGGGGCCCAAGTGATGCTCTGGGGTACTGGCGTGGGAGAGTGGATTTCCGAAGCTGACAGAT GGTTCATTGGAACAGAAAGAAATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCATTAATG CTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGTTGATCAAGGAACCTGTCTCCACAAAGTGTGA CCACATATTTTGCAAATTTTGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTA TGAGCCTACAAGAAAGTACGAGATTTAGTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCA GCTTGACACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCCTGAACAT CTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAAACCGTGCCAAAAGACTTCTACAGAGTG AACCCGAAAATCCTTCCTTGGAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCT GAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAATTGGGATCTGATTCTTCTGAA GATACCGTTAATAAGGCAACTTATTGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAA CCAGGGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGGATGTAACAAA TACTGAACATCATCAACCCAGTAATAATGATTTGAACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCA GAAAAGTATCAGGGTAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCT CATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTG TAATAAAAGCAAACAGCCTGGCTTAGCAAGGAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGT AATGATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAG AATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACT AAATAGCAGCATTCAGAAAGTTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCA CATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCTAAATGAGGTAGATGAAT ATTCTGGTTCTTCAGAGAAAATAGACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGA AAGAGTTCACTCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTATCGGAAGAAG GCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAATTATAGGAGCATTTGTTACTGAGCCACAGA TAATACAAGAGCGTCCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGA GGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGATAAATCAGGGAACTAACCAA ACGGAGCAGAATGGTCAAGTGATGAATATTACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTA TTCAGAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAACGAAAGCTGA ACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTAAATATCCACAATTCAAAAGCACCTAAAAAG AATAGGCTGAGGAGGAAGTCTTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAA GCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAAGAGATAAAGAAAAAAAAGTA CAACCAAATGCCAGTCAGGCACAGCAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCC AAGAAGAGTAACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGCGATACTTTCCCAGAGCTGAAGT TAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATACCAGTGAACTTAAAGAATTTGTCAATCCTAG CCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAA GATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGAGAGTAGCAGTATTTCATTGG TACCTGGTACTGATTATGGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAA AACAGAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAATTCATGGTTGT TCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATCCATTGGGACATGAAGTTAACCACAGTCGGG AAACAAGCATAGAAATGGAAGAAAGTGAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAA GCGCCAGTCATTTGCTCCGTTTTCAAATCCAGGAAATGCAGAAGAGGAATGTGCAACATTCTCTGCCCAC TCTGGGTCCTTAAAGAAACAAAGTCCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAA AGAATGAGTCTAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAA AGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTC AGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCAC CACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTGCTAGAGGAAAACTTTGAGGA ACATTCAATGTCACCTGAAAGAGAAATGGGAAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGT AATAACATTAGAGAAAATGTTTTTAAAGAAGCCAGCTCAAGCAATATTAATGAAGTAGGTTCCAGTACTA ATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAA CAGAGGGCCAAAATTGAATGCTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTT CCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGAAGTAGTTCAGACTGTTAATA CAGATTTCTCTCCATATCTGATTTCAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGT TTGTTCTGAGACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTTTGCTGAAAAT GACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCCAGAAAGGAGAGCTTAGCAGGAGTCCTAGCC CTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAA CTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCT TCTCAGTCTACTAGGCATAGCACCGTTGCTACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTAT CATTGAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGGAACATCACCT TAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCA AATACAAACACCCAGGATCCTTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGG GAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGAACGGGCTTGGAAGAAAATAA TCAAGAAGAGCAAAGCATGGATTCAAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTC TCTGAAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGATACCATGCAAC ATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCC TTCTAACAGCTACCCTTCCATCATAAGTGACTCTTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGC ACATCAGAAAAAGCAGTATTAACTTCACAGAAAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCC TTTCTGCTGACAAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGAAAG GTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGCACAGTTGCTCTGGGAGTCTTCAG AATAGAAACTACCCATCTCAAGAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGT CTGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAACCCCTTACCTGGA ATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCT CGTGTTGGCAACATACCATCTTCAACCTCTGCATTGAAAGTTCCCCAATTGAAAGTTGCAGAATCTGCCC AGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATGGAAGAAAGTGTGAGCAGGGA GAAGCCAGAATTGACAGCTTCAACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTG ACC CCA GAAGAATTTATGCTCGTGT ACAAGTTTGCCAGAAAACACCACATCACTTTAACTAATCTAATTACTG AAGAGACTACTCATGTTGTTATGAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCT AGGAATTGCGGGAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAAATG CTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAGAAACCACCAAGGTCCAAAGCGAG CAAGAGAATCCCAGGACAGAAAGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACAT GCCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGAGCTTTCATCATTC ACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCC ATGCAATTGGGCAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACTCTA CCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACAGCCACTACTGACTGCAGCCAGCC ACAGGTACAGAGCCACAGGACCCCAAGAATGAGCTTACAAAGTGGCCTTTCCAGGCCCTGGGAGCTCCTC TCACTCTTCAGTCCTTCTACTGTCCTGGCTACTAAATATTTTATGTACATCAGCCTGAAAAGGACTTCTG GCTATGCAAGGGTCCCTTAAAGATTTTCTGCTTGAAGTCTCCCTTGGAAATCTGCCATGAGCACAAAATT ATGGTAATTTTTCACCTGAGAAGATTTTAAAACCATTTAAACGCCACCAATTGAGCAAGATGCTGATTCA TTATTTATCAGCCCTATTCTTTCTATTCAGGCTGTTGTTGGCTTAGGGCTGGAAGCACAGAGTGGCTTGG CCTCAAGAGAATAGCTGGTTTCCCTAAGTTTACTTCTCTAAAACCCTGTGTTCACAAAGGCAGAGAGTCA GACCCTTCAATGGAAGGAGAGTGCTTGGGATCGATTATGTGACTTAAAGTCAGAATAGTCCTTGGGCAGT TCTCAAATGTTGGAGTGGAACATTGGGGAGGAAATTCTGAGGCAGGTATTAGAAATGAAAAGGAAACTTG AAACCTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACTGGA GGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAGAAATTAGC CGGTCATGGTGGTGGACACCTGTAATCCCAGCTACTCAGGTGGCTAAGGCAGGAGAATCACTTCAGCCCG GGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACGGCACTCCAGCCTGGGTGACAGTGAGACTGTGGC TCAAAAAAAAAAAAAAAAAAAGGAAAATGAAACTAGAAGAGATTTCTAAAAGTCTGAGATATATTTGCTA GATTTCTAAAGAATGTGTTCTAAAACAGCAGAAGATTTTCAAGAACCGGTTTCCAAAGACAGTCTTCTAA TTCCTCATTAGTAATAAGTAAAATGTTTATTGTTGTAGCTCTGGTATATAATCCATTCCTCTTAAAATAT AAGACCTCTGGCATGAATATTTCATATCTATAAAATGACAGATCCCACCAGGAAGGAAGCTGTTGCTTTC TTTGAGGTGATTTTTTTCCTTTGCTCCCTGTTGCTGAAACCATACAGCTTCATAAATAATTTTGCTTGCT GAAGGAAGAAAAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAGGACTAGGTC TTCCCTAGCCCCCCCAGTGTGCAAGGGCAGTGAAGACTTGATTGTACAAAATACGTTTTGTAAATGTTGT GCTGTTAACACTGCAAATAAACTTGGTAGCAAACACTTCCAAAAAAAAAAAAAAAAAA SEQ ID NO: 10  - Homo sapiens CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1, mRNA AGCGAGCTCCTCCTCCTTCCCCTCCCCACTCTCCCCGAGTCTAGGGCCCCCGGGGCGTATGACGCCGGAG CCCTCTGACCGCACCTCTGACCACAACAAACCCCTACTCCACCCGTCTTGTTTGTCCCACCCTTGGTGAC GCAGAGCCCCAGCCCAGACCCCGCCCAAAGCACTCATTTAACTGGTATTGCGGAGCCACGAGGCTTCTGC TTACTGCAACTCGCTCCGGCCGCTGGGCGTAGCTGCGACTCGGCGGAGTCCCGGCGGCGCGTCCTTGTTC TAACCCGGCGCGCCATGACCGTCGCGCGGCCGAGCGTGCCCGCGGCGCTGCCCCTCCTCGGGGAGCTGCC CCGGCTGCTGCTGCTGGTGCTGTTGTGCCTGCCGGCCGTGTGGGGTGACTGTGGCCTTCCCCCAGATGTA CCTAATGCCCAGCCAGCTTTGGAAGGCCGTACAAGTTTTCCCGAGGATACTGTAATAACGTACAAATGTG AAGAAAGCTTTGTGAAAATTCCTGGCGAGAAGGACTCAGTGATCTGCCTTAAGGGCAGTCAATGGTCAGA TATTGAAGAGTTCTGCAATCGTAGCTGCGAGGTGCCAACAAGGCTAAATTCTGCATCCCTCAAACAGCCT TATATCACTCAGAATTATTTTCCAGTCGGTACTGTTGTGGAATATGAGTGCCGTCCAGGTTACAGAAGAG AACCTTCTCTATCACCAAAACTAACTTGCCTTCAGAATTTAAAATGGTCCACAGCAGTCGAATTTTGTAA AAAGAAATCATGCCCTAATCCGGGAGAAATACGAAATGGTCAGATTGATGTACCAGGTGGCATATTATTT GGTGCAACCATCTCCTTCTCATGTAACACAGGGTACAAATTATTTGGCTCGACTTCTAGTTTTTGTCTTA TTTCAGGCAGCTCTGTCCAGTGGAGTGACCCGTTGCCAGAGTGCAGAGAAATTTATTGTCCAGCACCACC ACAAATTGACAATGGAATAATTCAAGGGGAACGTGACCATTATGGATATAGACAGTCTGTAACGTATGCA TGTAATAAAGGATTCACCATGATTGGAGAGCACTCTATTTATTGTACTGTGAATAATGATGAAGGAGAGT GGAGTGGCCCACCACCTGAATGCAGAGGAAAATCTCTAACTTCCAAGGTCCCACCAACAGTTCAGAAACC TACCACAGTAAATGTTCCAACTACAGAAGTCTCACCAACTTCTCAGAAAACCACCACAAAAACCACCACA CCAAATGCTCAAGCAACACGGAGTACACCTGTTTCCAGGACAACCAAGCATTTTCATGAAACAACCCCAA ATAAAGGAAGTGGAACCACTTCAGGTACTACCCGTCTT CTA TCTGGGCACACGTGTTTCACGT TGACAGG TTTGCTTGGGACGCTAGTAACCATGGGCTTGCTGACTTAGCCAAAGAAGAGTTAAGAAGAAAATACACAC AAGTATACAGACTGTTCCTAGTTTCTTAGACTTATCTGCATATTGGATAAAATAAATGCAATTGTGCTCT TCATTTAGGATGCTTTCATTGTCTTTAAGATGTGTTAGGAATGTCAACAGAGCAAGGAGAAAAAAGGCAG TCCTGGAATCACATTCTTAGCACACCTACACCTCTTGAAAATAGAACAACTTGCAGAATTGAGAGTGATT CCTTTCCTAAAAGTGTAAGAAAGCATAGAGATTTGTTCGTATTTAGAATGGGATCACGAGGAAAAGAGAA GGAAAGTGATTTTTTTCCACAAGATCTGTAATGTTATTTCCACTTATAAAGGAAATAAAAAATGAAAAAC ATTATTTGGATATCAAAAGCAAATAAAAACCCAATTCAGTCTCTTCTAAGCAAAATTGCTAAAGAGAGAT GAACCACATTATAAAGTAATCTTTGGCTGTAAGGCATTTTCATCTTTCCTTCGGGTTGGCAAAATATTTT AAAGGTAAAACATGCTGGTGAACCAGGGGTGTTGATGGTGATAAGGGAGGAATATAGAATGAAAGACTGA ATCTTCCTTTGTTGCACAAATAGAGTTTGGAAAAAGCCTGTGAAAGGTGTCTTCTTTGACTTAATGTCTT TAAAAGTATCCAGAGATACTACAATATTAACATAAGAAAAGATTATATATTATTTCTGAATCGAGATGTC CATAGTCAAATTTGTAAATCTTATTCTTTTGTAATATTTATTTATATTTATTTATGACAGTGAACATTCT GATTTTACATGTAAAACAAGAAAAGTTGAAGAAGATATGTGAAGAAAAATGTATTTTTCCTAAATAGAAA TAAATGATCCCATTTTTTGGTATCATGTAGTATGTGAAATTTATTCTTAAACGTGACTACTTTATTTCTA AATAAGAAATTCCCTACCTGCTTCCTACAAGCAGTTCAGAATGCCATGCCTTGGTTGTCCTAGTGTGAAT AATTTTCAGCTACTTTAAAATTATATTGTACTTTCTCAAGCATGTCATATCCTTTCCTATTAGAGTATCT ATATTACTTGTTACTGATTTACCTGAAGGCAATCTGATTAATTTCTAGGTTTTTACCATATTCTTGTCAT CTTGCCAATTACATTTTAAGTGTTAGACTAGACTAAGATGTACTAGTTGTATAGAATATAACTAGATTTA TTATGGCAATGTTTATTTTGTCATTTTGCTTCATCTGTTTTGTTGTTGAAGTACTTTAAATTTCATACGT TCATGGCATTTCACTGTAAAGACTTTAATGTGTATTTCTTAAAATAAAACTTTTTTTCCTCCTTAAAAAA AAAAAAAAAAAA SEQ ID NO: 11 - Homo sapiens cadherin 1, type 1, E-cadherin (epithelial) (CDH1), mRNA AGTGGCGTCGGAACTGCAAAGCACCTGTGAGCTTGCGGAAGTCAGTTCAGACTCCAGCCCGCTCCAGCCC GGCCCGACCCGACCGCACCCGGCGCCTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCC GCAGCCTCTCGGCGCTGCTGCTGCTGCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCA CCCTGGCTTTGACGCCGAGAGCTACACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGCGTCCTG GGCAGAGTGAATTTTGAAGATTGCACCGGTCGACAAAGGACAGCCTATTTTTCCCTCGACACCCGATTCA AAGTGGGCACAGATGGTGTGATTACAGTCAAAAGGCCTCTACGGTTTCATAACCCACAGATCCATTTCTT GGTCTACGCCTGGGACTCCACCTACAGAAAGTTTTCCACCAAAGTCACGCTGAATACAGTGGGGCACCAC CACCGCCC CCC GCCCCATCAGGCCTCCGTTTCT GGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCT CTCCTGGCCTCAGAAGACAGAAGAGAGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGG CCCATTTCCTAAAAACCTGGTTCAGATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTACAGCATC ACTGGCCAAGGAGCTGACACACCCCCTGTTGGTGTCTTTATTATTGAAAGAGAAACAGGATGGCTGAAGG TGACAGAGCCTCTGGATAGAGAACGCATTGCCACATACACTCTCTTCTCTCACGCTGTGTCATCCAACGG GAATGCAGTTGAGGATCCAATGGAGATTTTGATCACGGTAACCGATCAGAATGACAACAAGCCCGAATTC ACCCAGGAGGTCTTTAAGGGGTCTGTCATGGAAGGTGCTCTTCCAGGAACCTCTGTGATGGAGGTCACAG CCACAGACGCGGACGATGATGTGAACACCTACAATGCCGCCATCGCTTACACCATCCTCAGCCAAGATCC TGAGCTCCCTGACAAAAATATGTTCACCATTAACAGGAACACAGGAGTCATCAGTGTGGTCACCACTGGG CTGGACCGAGAGAGTTTCCCTACGTATACCCTGGTGGTTCAAGCTGCTGACCTTCAAGGTGAGGGGTTAA GCACAACAGCAACAGCTGTGATCACAGTCACTGACACCAACGATAATCCTCCGATCTTCAATCCCACCAC GTACAAGGGTCAGGTGCCTGAGAACGAGGCTAACGTCGTAATCACCACACTGAAAGTGACTGATGCTGAT GCCCCCAATACCCCAGCGTGGGAGGCTGTATACACCATATTGAATGATGATGGTGGACAATTTGTCGTCA CCACAAATCCAGTGAACAACGATGGCATTTTGAAAACAGCAAAGGGCTTGGATTTTGAGGCCAAGCAGCA GTACATTCTACACGTAGCAGTGACGAATGTGGTACCTTTTGAGGTCTCTCTCACCACCTCCACAGCCACC GTCACCGTGGATGTGCTGGATGTGAATGAAGCCCCCATCTTTGTGCCTCCTGAAAAGAGAGTGGAAGTGT CCGAGGACTTTGGCGTGGGCCAGGAAATCACATCCTACACTGCCCAGGAGCCAGACACATTTATGGAACA GAAAATAACATATCGGATTTGGAGAGACACTGCCAACTGGCTGGAGATTAATCCGGACACTGGTGCCATT TCCACTCGGGCTGAGCTGGACAGGGAGGATTTTGAGCACGTGAAGAACAGCACGTACACAGCCCTAATCA TAGCTACAGACAATGGTTCTCCAGTTGCTACTGGAACAGGGACACTTCTGCTGATCCTGTCTGATGTGAA TGACAACGCCCCCATACCAGAACCTCGAACTATATTCTTCTGTGAGAGGAATCCAAAGCCTCAGGTCATA AACATCATTGATGCAGACCTTCCTCCCAATACATCTCCCTTCACAGCAGAACTAACACACGGGGCGAGTG CCAACTGGACCATTCAGTACAACGACCCAACCCAAGAATCTATCATTTTGAAGCCAAAGATGGCCTTAGA GGTGGGTGACTACAAAATCAATCTCAAGCTCATGGATAACCAGAATAAAGACCAAGTGACCACCTTAGAG GTCAGCGTGTGTGACTGTGAAGGGGCCGCTGGCGTCTGTAGGAAGGCACAGCCTGTCGAAGCAGGATTGC AAATTCCTGCCATTCTGGGGATTCTTGGAGGAATTCTTGCTTTGCTAATTCTGATTCTGCTGCTCTTGCT GTTTCTTCGGAGGAGAGCGGTGGTCAAAGAGCCCTTACTGCCCCCAGAGGATGACACCCGGGACAACGTT TATTACTATGATGAAGAAGGAGGCGGAGAAGAGGACCAGGACTTTGACTTGAGCCAGCTGCACAGGGGCC TGGACGCTCGGCCTGAAGTGACTCGTAACGACGTTGCACCAACCCTCATGAGTGTCCCCCGGTATCTTCC CCGCCCTGCCAATCCCGATGAAATTGGAAATTTTATTGATGAAAATCTGAAAGCGGCTGATACTGACCCC ACAGCCCCGCCTTATGATTCTCTGCTCGTGTTTGACTATGAAGGAAGCGGTTCCGAAGCTGCTAGTCTGA GCTCCCTGAACTCCTCAGAGTCAGACAAAGACCAGGACTATGACTACTTGAACGAATGGGGCAATCGCTT CAAGAAGCTGGCTGACATGTACGGAGGCGGCGAGGACGACTAGGGGACTCGAGAGAGGCGGGCCCCAGAC CCATGTGCTGGGAAATGCAGAAATCACGTTGCTGGTGGTTTTTCAGCTCCCTTCCCTTGAGATGAGTTTC TGGGGAAAAAAAAGAGACTGGTTAGTGATGCAGTTAGTATAGCTTTATACTCTCTCCACTTTATAGCTCT AATAAGTTTGTGTTAGAAAAGTTTCGACTTATTTCTTAAAGCTTTTTTTTTTTTCCCATCACTCTTTACA TGGTGGTGATGTCCAAAAGATACCCAAATTTTAATATTCCAGAAGAACAACTTTAGCATCAGAAGGTTCA CCCAGCACCTTGCAGATTTTCTTAAGGAATTTTGTCTCACTTTTAAAAAGAAGGGGAGAAGTCAGCTACT CTAGTTCTGTTGTTTTGTGTATATAATTTTTTAAAAAAAATTTGTGTGCTTCTGCTCATTACTACACTGG TGTGTCCCTCTGCCTTTTTTTTTTTTTTAAGACAGGGTCTCATTCTATCGGCCAGGCTGGAGTGCAGTGG TGCAATCACAGCTCACTGCAGCCTTGTCCTCCCAGGCTCAAGCTATCCTTGCACCTCAGCCTCCCAAGTA GCTGGGACCACAGGCATGCACCACTACGCATGACTAATTTTTTAAATATTTGAGACGGGGTCTCCCTGTG TTACCCAGGCTGGTCTCAAACTCCTGGGCTCAAGTGATCCTCCCATCTTGGCCTCCCAGAGTATTGGGAT TACAGACATGAGCCACTGCACCTGCCCAGCTCCCCAACTCCCTGCCATTTTTTAAGAGACAGTTTCGCTC CATCGCCCAGGCCTGGGATGCAGTGATGTGATCATAGCTCACTGTAACCTCAAACTCTGGGGCTCAAGCA GTTCTCCCACCAGCCTCCTTTTTATTTTTTTGTACAGATGGGGTCTTGCTATGTTGCCCAAGCTGGTCTT AAACTCCTGGCCTCAAGCAATCCTTCTGCCTTGGCCCCCCAAAGTGCTGGGATTGTGGGCATGAGCTGCT GTGCCCAGCCTCCATGTTTTAATATCAACTCTCACTCCTGAATTCAGTTGCTTTGCCCAAGATAGGAGTT CTCTGATGCAGAAATTATTGGGCTCTTTTAGGGTAAGAAGTTTGTGTCTTTGTCTGGCCACATCTTGACT AGGTATTGTCTACTCTGAAGACCTTTAATGGCTTCCCTCTTTCATCTCCTGAGTATGTAACTTGCAATGG GCAGCTATCCAGTGACTTGTTCTGAGTAAGTGTGTTCATTAATGTTTATTTAGCTCTGAAGCAAGAGTGA TATACTCCAGGACTTAGAATAGTGCCTAAAGTGCTGCAGCCAAAGACAGAGCGGAACTATGAAAAGTGGG CTTGGAGATGGCAGGAGAGCTTGTCATTGAGCCTGGCAATTTAGCAAACTGATGCTGAGGATGATTGAGG TGGGTCTACCTCATCTCTGAAAATTCTGGAAGGAATGGAGGAGTCTCAACATGTGTTTCTGACACAAGAT CCGTGGTTTGTACTCAAAGCCCAGAATCCCCAAGTGCCTGCTTTTGATGATGTCTACAGAAAATGCTGGC TGAGCTGAACACATTTGCCCAATTCCAGGTGTGCACAGAAAACCGAGAATATTCAAAATTCCAAATTTTT TTCTTAGGAGCAAGAAGAAAATGTGGCCCTAAAGGGGGTTAGTTGAGGGGTAGGGGGTAGTGAGGATCTT GATTTGGATCTCTTTTTATTTAAATGTGAATTTCAACTTTTGACAATCAAAGAAAAGACTTTTGTTGAAA TAGCTTTACTGTTTCTCAAGTGTTTTGGAGAAAAAAATCAACCCTGCAATCACTTTTTGGAATTGTCTTG ATTTTTCGGCAGTTCAAGCTATATCGAATATAGTTCTGTGTAGAGAATGTCACTGTAGTTTTGAGTGTAT ACATGTGTGGGTGCTGATAATTGTGTATTTTCTTTGGGGGTGGAAAAGGAAAACAATTCAAGCTGAGAAA AGTATTCTCAAAGATGCATTTTTATAAATTTTATTAAACAATTTTGTTAAACCAT SEQ ID NO: 12 - Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mRNA CTTCTTCGTCAGCCTCCCTTCCACCGCCATATTGGGCCACTAAAAAAAGGGGGCTCGTCTTTTCGGGGTG TTTTTCTCCCCCTCCCCTGTCCCCGCTTGCTCACGGCTCTGCGACTCCGACGCCGGCAAGGTTTGGAGAG CGGCTGGGTTCGCGGGACCCGCGGGCTTGCACCCGCCCAGACTCGGACGGGCTTTGCCACCCTCTCCGCT TGCCTGGTCCCCTCTCCTCTCCGCCCTCCCGCTCGCCAGTCCATTTGATCAGCGGAGACTCGGCGGCCGG GCCGGGGCTTCCCCGCAGCCCCTGCGCGCTCCTAGAGCTCGGGCCGTGGCTCGTCGGGGTCTGTGTCTTT TGGCTCCGAGGGCAGTCGCTGGGCTTCCGAGAGGGGTTCGGGCTGCGTAGGGGCGCTTTGTTTTGTTCGG TTTTGTTTTTTTGAGAGTGCGAGAGAGGCGGTCGTGCAGACCCGGGAGAAAGATGTCAAACGTGCGAGTG TCTAACGGGAGCCCTAGCCTGGAGCGGATGGACGCCAGGCAGGCGGAGCACCCCAAGCCCTCGGCCTGCA GGAACCTCTTCGGCCCGGTGGACCACGAAGAGTTAACCCGGGACTTGGAGAAGCACTGCAGAGACATGGA AGAGGCGAGCCAGCGCAAGTGGAATTTCGATTTTCAGAATCACAAACCCCTAGAGGGCAAGTACGAGTGG CAAGAGGTGGAGAAGGGCAGCTTGCCCGAGTTCTACTACAGACCCCCGCGGCCCCCCAAAGGTGCCTGCA AGGTGCCGGCGCAGGAGAGCCAGGATGTCAGCGGGAGCCGCCCGGCGGCGCCTTTAATTGGGGCTCCGGC TAACTCTGAGGACACGCATTTGGTGGACCCAAAGACTGATCCGTCGGACAGCCAGACGGGGTTAGCGGAG CAATGCGCAGGAATAAGGAAGCGACCT GCA ACCGACGATTCTTCTACTCAAA ACAAAAGAGCCAACAGAA CAGAAGAAAATGTTTCAGACGGTTCCCCAAATGCCGGTTCTGTGGAGCAGACGCCCAAGAAGCCTGGCCT CAGAAGACGTCAAACGTAAACAGCTCGAATTAAGAATATGTTTCCTTGTTTATCAGATACATCACTGCTT GATGAAGCAAGGAAGATATACATGAAAATTTTAAAAATACATATCGCTGACTTCATGGAATGGACATCCT GTATAAGCACTGAAAAACAACAACACAATAACACTAAAATTTTAGGCACTCTTAAATGATCTGCCTCTAA AAGCGTTGGATGTAGCATTATGCAATTAGGTTTTTCCTTATTTGCTTCATTGTACTACCTGTGTATATAG TTTTTACCTTTTATGTAGCACATAAACTTTGGGGAAGGGAGGGCAGGGTGGGGCTGAGGAACTGACGTGG AGCGGGGTATGAAGAGCTTGCTTTGATTTACAGCAAGTAGATAAATATTTGACTTGCATGAAGAGAAGCA ATTTTGGGGAAGGGTTTGAATTGTTTTCTTTAAAGATGTAATGTCCCTTTCAGAGACAGCTGATACTTCA TTTAAAAAAATCACAAAAATTTGAACACTGGCTAAAGATAATTGCTATTTATTTTTACAAGAAGTTTATT CTCATTTGGGAGATCTGGTGATCTCCCAAGCTATCTAAAGTTTGTTAGATAGCTGCATGTGGCTTTTTTA AAAAAGCAACAGAAACCTATCCTCACTGCCCTCCCCAGTCTCTCTTAAAGTTGGAATTTACCAGTTAATT ACTCAGCAGAATGGTGATCACTCCAGGTAGTTTGGGGCAAAAATCCGAGGTGCTTGGGAGTTTTGAATGT TAAGAATTGACCATCTGCTTTTATTAAATTTGTTGACAAAATTTTCTCATTTTCTTTTCACTTCGGGCTG TGTAAACACAGTCAAAATAATTCTAAATCCCTCGATATTTTTAAAGATCTGTAAGTAACTTCACATTAAA AAATGAAATATTTTTTAATTTAAAGCTTACTCTGTCCATTTATCCACAGGAAAGTGTTATTTTTCAAGGA AGGTTCATGTAGAGAAAAGCACACTTGTAGGATAAGTGAAATGGATACTACATCTTTAAACAGTATTTCA TTGCCTGTGTATGGAAAAACCATTTGAAGTGTACCTGTGTACATAACTCTGTAAAAACACTGAAAAATTA TACTAACTTATTTATGTTAAAAGATTTTTTTTAATCTAGACAATATACAAGCCAAAGTGGCATGTTTTGT GCATTTGTAAATGCTGTGTTGGGTAGAATAGGTTTTCCCCTCTTTTGTTAAATAATATGGCTATGCTTAA AAGGTTGCATACTGAGCCAAGTATAATTTTTTGTAATGTGTGAAAAAGATGCCAATTATTGTTACACATT AAGTAATCAATAAAGAAAACTTCCATAGCTATT SEQ ID No: 13 - Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 3, mRNA GCAGGTTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCG TGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGG CAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATG CCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGG AACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTG GGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAGACAGAGTCTGGCCATGTTACCCAATCT GATCTTGAACTCCTGCTGTCATCTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATAAGAGGTGTGA GGCACCATCCCCGGCCAGTTTGCAGTCTAAAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAG CTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACAC TTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATG GAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGC ACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCT AAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGG CTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTC AGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCT TGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATA TTG TTTTGGAATTGATGGAAGGGG G AGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCA GATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTT TTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAG AGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGG GACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGG TATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACT TCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGA TCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGA AAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTA CTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGC TGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTT TCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATG TACAATTAAAAACCTGATGGAACCTGGAAAA SEQ ID NO: 14 - Homo sapiens colony stimulating factor 3 receptor (granulocyte) (CSF3R), transcript variant 3, mRNA GAGTACTGTGAAGATGTGGTCCCCAAGGCTAGAGCTGAAAAGAGGCTTAGGGCCGGGTGAGCCTTCCAGC CAGGGCCTGCCTCCAAGTGATGCTCCCCCAGGGCAGGGGGCATAAGGATGGCACCCAGCCAGGTGGGAGC CTGGGCCCTGCCCAGCCTCAAAGCTTTGAGCTCAGGAAATCCGGAGGCAGGGGAGGGGGACATCGTTGCC ACATTCCCCAGCCCTTTAAGACCCCCAAGGCAGGAAGGCTGCCCGGGCCTCACCAGCTTCCCTCACAGGC TCCTTCCTGGGAGGAAGGGGCTGCCTGTGCCCTCGAAGGCGCAAGGGAGGGCAGGAGGGAGGCTCGGAAG GTGTTGCAATCCCCAGCCCCCGGGCCTGTCAGAGGCTGAGCCATTAACGACAGAGCTCGGGGAGAGAAGC TGGACTGCAGCTGGTTTCAGGAACTTCTCTTGACGAGAAGAGAGACCAAGGAGGCCAAGCAGGGGCTGGG CCAGAGGTGCCAACATGGGGAAACTGAGGCTCGGCTCGGAAAGGTGAAGTAACTTGTCCAAGATCACAAA GCTGGTGAACATCAAGTTGGTGCTATGGCAAGGCTGGGAAACTGCAGCCTGACTTGGGCTGCCCTGATCA TCCT GCT GCTCCCCGGAAGTCTGGAGGAG TGCGGGCACATCAGTGTCTCAGCCCCCATCGTCCACCTGGG GGATCCCATCACAGCCTCCTGCATCATCAAGCAGAACTGCAGCCATCTGGACCCGGAGCCACAGATTCTG TGGAGACTGGGAGCAGAGCTTCAGCCCGGGGGCAGGCAGCAGCGTCTGTCTGATGGGACCCAGGAATCTA TCATCACCCTGCCCCACCTCAACCACACTCAGGCCTTTCTCTCCTGCTGCCTGAACTGGGGCAACAGCCT GCAGATCCTGGACCAGGTTGAGCTGCGCGCAGGCTACCCTCCAGCCATACCCCACAACCTCTCCTGCCTC ATGAACCTCACAACCAGCAGCCTCATCTGCCAGTGGGAGCCAGGACCTGAGACCCACCTACCCACCAGCT TCACTCTGAAGAGTTTCAAGAGCCGGGGCAACTGTCAGACCCAAGGGGACTCCATCCTGGACTGCGTGCC CAAGGACGGGCAGAGCCACTGCTGCATCCCACGCAAACACCTGCTGTTGTACCAGAATATGGGCATCTGG GTGCAGGCAGAGAATGCGCTGGGGACCAGCATGTCCCCACAACTGTGTCTTGATCCCATGGATGTTGTGA AACTGGAGCCCCCCATGCTGCGGACCATGGACCCCAGCCCTGAAGCGGCCCCTCCCCAGGCAGGCTGCCT ACAGCTGTGCTGGGAGCCATGGCAGCCAGGCCTGCACATAAATCAGAAGTGTGAGCTGCGCCACAAGCCG CAGCGTGGAGAAGCCAGCTGGGCACTGGTGGGCCCCCTCCCCTTGGAGGCCCTTCAGTATGAGCTCTGCG GGCTCCTCCCAGCCACGGCCTACACCCTGCAGATACGCTGCATCCGCTGGCCCCTGCCTGGCCACTGGAG CGACTGGAGCCCCAGCCTGGAGCTGAGAACTACCGAACGGGCCCCCACTGTCAGACTGGACACATGGTGG CGGCAGAGGCAGCTGGACCCCAGGACAGTGCAGCTGTTCTGGAAGCCAGTGCCCCTGGAGGAAGACAGCG GACGGATCCAAGGTTATGTGGTTTCTTGGAGACCCTCAGGCCAGGCTGGGGCCATCCTGCCCCTCTGCAA CACCACAGAGCTCAGCTGCACCTTCCACCTGCCTTCAGAAGCCCAGGAGGTGGCCCTTGTGGCCTATAAC TCAGCCGGGACCTCTCGTCCCACTCCGGTGGTCTTCTCAGAAAGCAGAGGCCCAGCTCTGACCAGACTCC ATGCCATGGCCCGAGACCCTCACAGCCTCTGGGTAGGCTGGGAGCCCCCCAATCCATGGCCTCAGGGCTA TGTGATTGAGTGGGGCCTGGGCCCCCCCAGCGCGAGCAATAGCAACAAGACCTGGAGGATGGAACAGAAT GGGAGAGCCACGGGGTTTCTGCTGAAGGAGAACATCAGGCCCTTTCAGCTCTATGAGATCATCGTGACTC CCTTGTACCAGGACACCATGGGACCCTCCCAGCATGTCTATGCCTACTCTCAAGAAATGGCTCCCTCCCA TGCCCCAGAGCTGCATCTAAAGCACATTGGCAAGACCTGGGCACAGCTGGAGTGGGTGCCTGAGCCCCCT GAGCTGGGGAAGAGCCCCCTTACCCACTACACCATCTTCTGGACCAACGCTCAGAACCAGTCCTTCTCCG CCATCCTGAATGCCTCCTCCCGTGGCTTTGTCCTCCATGGCCTGGAGCCCGCCAGTCTGTATCACATCCA CCTCATGGCTGCCAGCCAGGCTGGGGCCACCAACAGTACAGTCCTCACCCTGATGACCTTGACCCCAGAG GGGTCGGAGCTACACATCATCCTGGGCCTGTTCGGCCTCCTGCTGTTGCTCACCTGCCTCTGTGGAACTG CCTGGCTCTGTTGCAGCCCCAACAGGAAGAATCCCCTCTGGCCAAGTGTCCCAGACCCAGCTCACAGCAG CCTGGGCTCCTGGGTGCCCACAATCATGGAGGAGCTGCCCGGACCCAGACAGGGACAGTGGCTGGGGCAG ACATCTGAAATGAGCCGTGCTCTCACCCCACATCCTTGTGTGCAGGATGCCTTCCAGCTGCCCGGCCTTG GCACGCCACCCATCACCAAGCTCACAGTGCTGGAGGAGGATGAAAAGAAGCCGGTGCCCTGGGAGTCCCA TAACAGCTCAGAGACCTGTGGCCTCCCCACTCTGGTCCAGACCTATGTGCTCCAGGGGGACCCAAGAGCA GTTTCCACCCAGCCCCAATCCCAGTCTGGCACCAGCGATCAGGTCCTTTATGGGCAGCTGCTGGGCAGCC CCACAAGCCCAGGGCCAGGGCACTATCTCCGCTGTGACTCCACTCAGCCCCTCTTGGCGGGCCTCACCCC CAGCCCCAAGTCCTATGAGAACCTCTGGTTCCAGGCCAGCCCCTTGGGGACCCTGGTAACCCCAGCCCCA AGCCAGGAGGACGACTGTGTCTTTGGGCCACTGCTCAACTTCCCCCTCCTGCAGGGGATCCGGGTCCATG GGATGGAGGCGCTGGGGAGCTTCTAGGGCTTCCTGGGGTTCCCTTCTTGGGCCTGCCTCTTAAAGGCCTG AGCTAGCTGGAGAAGAGGGGAGGGTCCATAAGCCCATGACTAAAAACTACCCCAGCCCAGGCTCTCACCA TCTCCAGTCACCAGCATCTCCCTCTCCTCCCAATCTCCATAGGCTGGGCCTCCCAGGCGATCTGCATACT TTAAGGACCAGATCATGCTCCATCCAGCCCCACCCAATGGCCTTTTGTGCTTGTTTCCTATAACTTCAGT ATTGTAAACTAGTTTTTGGTTTGCAGTTTTTGTTGTTGTTTATAGACACTCTTGGGTGTAAAAAAAAAAA SEQ ID NO: 15 - CYHomo sapiens cathepsin S (CTSS), transcript variant 1, mRNA GACAAGGGCTCTTCTTGATGGCTTACTGTATCCACTTTGTCCCCAAGACCATAGGGAAATGACTAGAGGT GACTGTACTAGCTAGATTTTAAATGAAACTGAAATGAAAGTTCACTTCCTCATTTTGAGTACCTCATGTG ACAAGTTCCAATTTCTTTTCAAGTCAATTGAACTGAAATCTCCTTGTTGCTTTGAAATCTTAGAAGAGAG CCCACTAATTCAAGGACTCTTACTGTGGGAGCAACTGCTGGTTCTATCACAATGAAACGGCTGGTTTGTG TGCTCTTGGTGTGCTCCTCTGCAGTGGCACAGTTGCATAAAGATCCTACCCTGGATCACCACTGGCATCT CTGGAAGAAAACCTATGGCAAACAATACAAGGAAAAGAATGAAGAAGCAGTACGACGTCTCATCTGGGAA AAGAATCTAAAGTTTGTGATGCTTCACAACCTGGAGCATTCAATGGGAATGCACTCATACGATCTGGGCA TGAACCA CCT GGGAGACATGACCAGTGAAGAA GTGATGTCTTTGATGAGTTCCCTGAGAGTTCCCAGCCA GTGGCAGAGAAATATCACATATAAGTCAAACCCTAATCGGATATTGCCTGATTCTGTGGACTGGAGAGAG AAAGGGTGTGTTACTGAAGTGAAATATCAAGGTTCTTGTGGTGCTTGCTGGGCTTTCAGTGCTGTGGGGG CCCTGGAAGCACAGCTGAAGCTGAAAACAGGAAAGCTGGTGTCTCTCAGTGCCCAGAACCTGGTGGATTG CTCAACTGAAAAATATGGAAACAAAGGCTGCAATGGTGGCTTCATGACAACGGCTTTCCAGTACATCATT GATAACAAGGGCATCGACTCAGACGCTTCCTATCCCTACAAAGCCATGGATCAGAAATGTCAATATGACT CAAAATATCGTGCTGCCACATGTTCAAAGTACACTGAACTTCCTTATGGCAGAGAAGATGTCCTGAAAGA AGCTGTGGCCAATAAAGGCCCAGTGTCTGTTGGTGTAGATGCGCGTCATCCTTCTTTCTTCCTCTACAGA AGTGGTGTCTACTATGAACCATCCTGTACTCAGAATGTGAATCATGGTGTACTTGTGGTTGGCTATGGTG ATCTTAATGGGAAAGAATACTGGCTTGTGAAAAACAGCTGGGGCCACAACTTTGGTGAAGAAGGATATAT TCGGATGGCAAGAAATAAAGGAAATCATTGTGGGATTGCTAGCTTTCCCTCTTACCCAGAAATCTAGAGG ATCTCTCCTTTTTATAACAAATCAAGAAATATGAAGCACTTTCTCTTAACTTAATTTTTCCTGCTGTATC CAGAAGAAATAATTGTGTCATGATTAATGTGTATTTACTGTACTAATTAGAAAATATAGTTTGAGGCCGG GCACGGTGGCTCACGCCTGTAATCCCAGTACTTGGGAGGCCAAGGCAGGCATATCAACTTGAGGCCAGGA GTTAAAGAGCAGCCTGGCTAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAAATTAGCCGAGCAC GGTGGTGCATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCACGAGATTCCTTGAACCCAAGAGGTTG AGGCTATGTTGAGCTGAGATCACACCACTGTACTCCAGCCTGGATGACAGAGTGGAGACTCTGTTTCAAA AAAACAGAAAAGAAAATATAGTTTGATTCTTCATTTTTTTAAATTTGCAAATCTCAGGATAAAGTTTGCT AAGTAAATTAGTAATGTACTATAGATATAACTGTACAAAAATTGTTCAACCTAAAACAATCTGTAATTGC TTATTGTTTTATTGTATACTCTTTGTCTTTTTAAGACCCCTAATAGCCTTTTGTAACTTGATGGCTTAAA AATACTTAATAAATCTGCCATTTCAAATTTCTATCATTGCCACATACCATTCTTATTCCTAGGCAACTAT TAATAATCTATCCTGAGAATATTAATTGTGGTATTCTGGTGATGGGGTTTAGCAACTTTGATGGAAGAAA ATATTAGGCTATAAATGTCCTAAGGACTCAGATTGTATCTTTGTACAGAAGAGGATTCAAAACGCCACGT GTAGTGGCTCATGCCTGTAATCCCAACACTTTGGGAGGCTGAAGTAGGAGGATCGTCTTGAGCCCAGGAG TTCAAGACCAGCCTGGACAACATAGTGAGACCTTGTCTCCACAAAAATAAAAAAGAAACTATCCAGGAGT GGTGGTGTGTGCCTGTGGTCCCTGCTATGCAGATGTCTAAGACAGGAGGATCACAAGAGCCCAGGAGGTT GAGAATGCAGTGAGCTTGTAATTGCACCACTGCACTCCAGCCTGGGTGACAGAGCAAGACCCTGTCTTAA AAAAAGAGGATTCAACACATATTTTTATATTATGTTAAAGTAAAGAAATGCATAAAAGACAAGCACTTTG GAAGAATTATTTTAATGATCAACAATTTAATGTATTAGTCCAAATTATTTTTACGTAGTCATCAACAATT TGACCAGGGCCTTTATTTGGCAAATAACTGAGCCAACCAGAATAAAATAACCAATACTCCACTGCTCATA TTTTTATCTAATTCAGATGGATCTTCCTTACAACTGCTCTAGATTAGTAGATGCATCTAAGCAGGCAGCA GGAACTTTAAATTTTTTAAGTTCATGTCTATGACATGAACAATGTGTGGGATAATGTCATTAATATATCC TAAATTAACCTAAACGTATTTCACTAACTCTGGCTCCTTCTCCATAAAGCACATTTTAAGGAACAAGAAT TGCTAAATATAAAAACATAAATAATACCATAATACATGGCTATCATCAAAAGTGTATAGAATATTATAGT TTAAAAGTATTTAGTTGATTACTTTTCAGTTTTGTTTTGTTTTTTGAGACGGAGTCTCACTCTGTTGCCC AGGCTGGAGTGCAGTGGCACCATCTCAGTTCACTGCAACTTCTGCCTCCCGAGTTCAAGCGATTCTCCTG CCTCAGCCTCCCGAGTAGCTGGAATTATAGGCGTGCACCACCACGCCCAGCTAATTTTTGTATTTTTAGT AAAGACAGGGTTTTGCCACATTAGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCCCA GCCTCCCAAAGTGCTAAGATTACAGGCGTGAGCCACTGAGCCCAGCCTACTTTTCAGTTTTTAACATAAT TTTTGTTTTATCCACAACTTTTCAAGTATTGAAAGTAGAATAAAAACATGGGTTCTTAGTCTTTAGCTAT CTGTTAAAGCCTATGAATGCCTTCTTAAAATCATGTTTTTAAATGCATAAAATATATAGGATTACAAAGG AATCTAATTATATCGAAATACAGTTATTAAAATGTTAAAAGATAAGTTTGTTATATATTAATATGCATGC TTCTTTATAAATGCATTAAATAAGAGTTAATAGCTATCCTAAATTTGAAATAGTGATAAGCATAATGAAA ATAGATGCAAAAAACTAATGTGATATGAAAATATCTGGGTTTTTCTTTTGATGATGAAGTATTGCTAATA TTACCGTGGTTTATGAACTATGTTCAGAATTGAAGAAAATCCTAACTTTCAGTTAGAGGTTAGTGACGGG GTTCAGGACACCCTACACAAAATACAGCACTTTGACATATTGAATATTTTAAGCTGAAGGCATTTGAGGA AATTGCAGAAGCAGGAAGGTGACTCTGACCTTCTGCCTGCTGTTCTCCCCAGAAGCAGCCATAAAACCTG GGAAGGATTTTCTGACCTTCCCCTGAAGTAGATCATAAGACTGTCATGTAAGAGGTGCTCTCCTGGCACC CAGAGAAAAGGAGCATCCTTACCTCCAAAAGCACAGGGACACAAAGAGGAATCTAAACAAACAGGCCTCT CAGTTTCCCCCAGTTTATTACATTTAGCTTGTTCACACTTTGCCCTATGACATTTCTACATCACTGGCTG CTCTTCATCAAACCTACTATAAAAAACATTCAAGTTCAACTGTTTCTTTGGGCCTTTATTTCCTTATGGA GCCCCTCGTGTCGTGTAAAACTTATATTAAATAAATGTGCATGCTTT SEQ ID NO: 16 - Homo sapiens epoxide hydrolase 2, cytoplasmic (EPHX2), mRNA CTGGGCGGGTCATGCGCCCTGGCCTTCGCGCATCTCCCAGGTTAGCTGCGTGTCCGGGTGCTAGGCTGCA GACCCGCCGCCATGACGCTGCGCGCGGCCGTCTTCGACCTTGACGGGGTGCTGGCGCTGCCAGCGGTGTT CGGCGTCCTCGGCCGCACGGAGGAGGCCCTGGCGCTGCCCAGAGGACTTCTGAATGATGCTTTCCAGAAA GGGGGACCAGAGGGTGCCACTACCCGGCTTATGAAAGGAGAGATCACACTTTCCCAGTGGATACCACTCA TGGAAGAAAACTGCAGGAAGTGCTCCGAGACCGCTAAAGTCTGCCTCCCCAAGAATTTCTCCATAAAAGA AATCTTTGACAAGGCGATTTCAGCCAGAAAGATCAACCGCCCCATGCTCCAGGCAGCTCTCATGCTCAGG AAGAAAGGATTCACTACTGCCATCCTCACCAACACCTGGCTGGACGACCGTGCTGAGAGAGATGGCCTGG CCCAGCTGATGTGTGAGCTGAAGATGCACTTTGACTTCCTGATAGAGTCGTGTCAGGTGGGAATGGTCAA ACCTGAACCTCAGATCTACAAGTTTCTGCTGGACACCCTGAAGGCCAGCCCCAGTGAGGTCGTTTTTTTG GATGACATCGGGGCTAATCTGAAGCCAGCCCGTGACTTGGGAATGGTCACCATCCTGGTCCAGGACACTG ACACGGCCCTGAAAGAACTGGAGAAAGTGACCGGAATCCAGCTTCTCAATACCCCGGCCCCTCTGCCGAC CTCTTGCAATCCAAGTGACATGAGCCATGGGT ACG TGACAGTAAAGCCCAGGGTCCG TCTGCATTTTGTG GAGCTGGGCTCCGGCCCTGCTGTGTGCCTCTGCCATGGATTTCCCGAGAGTTGGTATTCTTGGAGGTACC AGATCCCTGCTCTGGCCCAGGCAGGTTACCGGGTCCTAGCTATGGACATGAAAGGCTATGGAGAGTCATC TGCTCCTCCCGAAATAGAAGAATATTGCATGGAAGTGTTATGTAAGGAGATGGTAACCTTCCTGGATAAA CTGGGCCTCTCTCAAGCAGTGTTCATTGGCCATGACTGGGGTGGCATGCTGGTGTGGTACATGGCTCTCT TCTACCCCGAGAGAGTGAGGGCGGTGGCCAGTTTGAATACTCCCTTCATACCAGCAAATCCCAACATGTC CCCTTTGGAGAGTATCAAAGCCAACCCAGTATTTGATTACCAGCTCTACTTCCAAGAACCAGGAGTGGCT GAGGCTGAACTGGAACAGAACCTGAGTCGGACTTTCAAAAGCCTCTTCAGAGCAAGCGATGAGAGTGTTT TATCCATGCATAAAGTCTGTGAAGCGGGAGGACTTTTTGTAAATAGCCCAGAAGAGCCCAGCCTCAGCAG GATGGTCACTGAGGAGGAAATCCAGTTCTATGTGCAGCAGTTCAAGAAGTCTGGTTTCAGAGGTCCTCTA AACTGGTACCGAAACATGGAAAGGAACTGGAAGTGGGCTTGCAAAAGCTTGGGACGGAAGATCCTGATTC CGGCCCTGATGGTCACGGCGGAGAAGGACTTCGTGCTCGTTCCTCAGATGTCCCAGCACATGGAGGACTG GATTCCCCACCTGAAAAGGGGACACATTGAGGACTGTGGGCACTGGACACAGATGGACAAGCCAACCGAG GTGAATCAGATCCTCATTAAGTGGCTGGATTCTGATGCCCGGAACCCACCGGTGGTCTCAAAGATGTAGA ACGCAGCGTGTGCCCACGCTCAGCAGGTGTGCCATCCTTCCACCTGCTGGGGCACCATTCTTAGTATACA GAGGTGGCCTTACACACATCTTGCATGGATGGCAGCATTGTTCTGAAGGGGTTTGCAGAAAAAAAAGATT TTCTTTACATAAAGTGAATCAAATTTGACATTATTTTAGATCCCAGAGAAATCAGGTGTGATTAGTTCTC CAGGCATGAATGCATCGTCCCTTTATCTGTAAGAACCCTTAGTGTCCTGTAGGGGGACAGAATGGGGTGG CCAGGTGGTGATTTCTCTTTGACCAATGCATAGTTTGGCAGAAAAATCAGCCGTTCATTTAGAAGAATCT TAGCAGAGATTGGGATGCCTTACTCAATAAAGCTAAGATGACTATGCTGCTGGCTGTCTTTGTTCTTGGA GAGGTGGAGTGACTGTTCACGGAGAA SEQ ID NO: 17 - Homo sapiens exostosin 2 (EXT2), transcript variant 2, mRNA CTGTCTGAGCATTTCACTGCGGAGCCTGAGCGCGCCTGCCTGGGAAAACACTGCAGCGGTGCTCGGACTC CTCCTGTCCAGCAGGAGGCGCGGCCCGGCAGCTCCCGCATGCGCAGTGCGCTCGGTGTCAGACGGCCCGG ATCCCGGTTACCGGCCCCTCGCTCGCTGCTCGCCAGCCCAGACTCGGCCCTGGCAGTGGCGGCTGGCGAT TCGGACCGATCCGACCTGGGCGGAGGTGGCCCGCGCCCCGCGGCATGAGCCGGTGACCAAGCTCGGGGCC GAGCGGGAGGCAGCCGTGGCCGAGGAGTGTGAGGAAGAGGCTGTCTGTGTCATTATGTGTGCGTCGGTCA AGTATAATATCCGGGGTCCTGCCCTCATCCCAAGAATGAAGACCAAGCACCGAATCTACTATATCACCCT CTTCTCCATTGTCCTCCTGGGCCTCATTGCCACTGGCATGTTTCAGTTTTGGCCCCATTCTATCGAGTCC TCAAATGACTGGAATGTAGAGAAGCGCAGCATCCGTGATGTGCCGGTTGTTAGGCTGCCAGCCGACAGTC CCATCCCAGAGCGGGGGGATCTCAGTTGCAGAATGCACACGTGTTTTGATGTCTATCGCTGTGGCTTCAA CCCAAAGAACAAAATCAAGGTGTATATCTATGCTCTGAAAAAGTACGTGGATGACTTTGGCGTCTCTGTC AGCAACACCATCTCCCGGGAGTATAATGAACTGCTCATGGCCATCTCAGACAGTGACTACTACACTGATG ACATCAACCGGGCCTGTCTGTTTGTTCCCTCCATCGATGTGCTTAACCAGAACACACTGCGCATCAAGGA GACAGCACAAGCGATGGCCCAGCTCTCTAGGTGGGATCGAGGTACGAATCACCTGTTGTTCAACATGTTG CCTGGAGGTCCCCCAGATTATAACACAGCCCTGGATGTCCCCAGAGACAGGGCCCTGTTGGCTGGTGGCG GCTTTTCTACGTGGACTTACCGGCAAGGCTACGATGTCAGCATTCCTGTCTATAGTCCACTGTCAGCTGA GGTGGATCTTCCAGAGAAAGGACCAGGTCCACGGCAATACTTCCTCCTGTCATCTCAGGTGGGTCTCCAT CCTGAGTACAGAGAGGACCTAGAAGCCCTCCAGGTCAAACATGGAGAGTCAGTGTTAGTACTCGATAAAT GCACCAACCTCTCAGAGGGTGTCCTTTCTGTCCGTAAGCGCTGCCACAAGCACCAGGTCTTCGATTACCC ACAGGTGCTACAGGAGGCTACTTTCTGTGTGGTTCTTCGTGGAGCTCGGCTGGGCCAGGCAGTATTGAGC GATGTGTTACAAGCTGGCTGTGTCCCGGTTGTCATTGCAGACTCCTATATTTTGCCTTTCTCTGAAGTTC TTGACTGGAAGAGAGCATCTGTGGTTGTACCAGAAGAAAAGATGTCAGATGTGTACAGTATTTTGCAGAG CATCCCCCAAAGACAGATTGAAGAAATGCAGAGACAGGCCCGCTGGTTCTGGGAAGCGTACTTCCAGTCA ATTAAAGCCATTGCCCTGGCCACCCTGCAGATTATCAATGACCGGATCTATCCATATGCTGCCATCTCCT ATGAAGAATGGAATGA CCC TCCTGCTGTGAAGTGGGGCAGC GTGAGCAATCCACTCTTCCTCCCGCTGAT CCCACCACAGTCTCAAGGGTTCACCGCCATAGTCCTCACCTACGACCGAGTAGAGAGCCTCTTCCGGGTC ATCACTGAAGTCTCCAAGGTGCCCAGTCTATCCAAACTACTTCTCGTCTGGAATAATCAGAATAAAAACC CTCCAGAAGATTCTCTCTGGCCCAAAATCCGGGTTCCATTAAAAGTTGTGAGGACTGCTGAAAACAAGTT AAGTAACCGTTTCTTCCCTTATGATGAAATCGAGACAGAAGCTGTTCTGGCCATTGATGATGATATCATT ATGCTGACCTCTGACGAGCTGCAATTTGGTTATGAGGTCTGGCGGGAATTTCCTGACCGGTTGGTGGGTT ACCCGGGTCGTCTGCATCTCTGGGACCATGAGATGAATAAGTGGAAGTATGAGTCTGAGTGGACGAATGA AGTGTCCATGGTGCTCACTGGGGCAGCTTTTTATCACAAGTATTTTAATTACCTGTATACCTACAAAATG CCTGGGGATATCAAGAACTGGGTAGATGCTCATATGAACTGTGAAGATATTGCCATGAACTTCCTGGTGG CCAACGTCACGGGAAAAGCAGTTATCAAGGTAACCCCACGAAAGAAATTCAAGTGTCCTGAGTGCACAGC CATAGATGGGCTTTCACTAGACCAAACACACATGGTGGAGAGGTCAGAGTGCATCAACAAGTTTGCTTCA GTCTTCGGGACCATGCCTCTCAAGGTGGTGGAACACCGAGCTGACCCTGTCCTGTACAAAGATGACTTTC CTGAGAAGCTGAAGAGCTTCCCCAACATTGGCAGCTTATGAAACGTGTCATTGGTGGAGGTCTGAATGTG AGGCTGGGACAGAGGGAGAGAACAAGGCCTCCCAGCACTCTGATGTCAGAGTAGTAGGTTAAGGGTGGAA GGTTGACCTACTTGGATCTTGGCATGCACCCACCTAACCCACTTTCTCAAGAACAAGAACCTAGAATGAA TATCCAAGCACCTCGAGCTATGCAACCTCTGTTCTTGTATTTCTTATGATCTCTGATGGGTTCTTCTCGA AAATGCCAAGTGGAAGACTTTGTGGCATGCTCCAGATTTAAATCCAGCTGAGGCTCCCTTTGTTTTCAGT TCCATGTAACAATCTGGAAGGAAACTTCACGGACAGGAAGACTGCTGGAGAAGAGAAGCGTGTTAGCCCA TTTGAGGTCTGGGGAATCATGTAAAGGGTACCCAGACCTCACTTTTAGTTATTTACATCAATGAGTTCTT TCAGGGAACCAAACCCAGAATTCGGTGCAAAAGCCAAACATCTTGGTGGGATTTGATAAATGCCTTGGGA CCTGGAGTGCTGGGCTTGTGCACAGGAAGAGCACCAGCCGCTGAGTCAGGATCCTGTCAGTTCCATGAGC TATTCCTCTTTGGTTTGGCTTTTTGATATGATTAAAATTATTTTTTATTCCTTTTTCTACTGTGTCTTAA ACACCAATTCCTGATAGTCCAAGGAACCACCTTTCTCCCTTGATATATTTAACTCCGTCTTTGGCCTGAC AACAGTCTTCTGCCCATGTCTGGGAACACACGCCAGGAGGAATGTCTGATACCCTCTGCATCAAGCGTAA GAAGGTCCCAAATCATAACCATTTTAAGAACAGATGACTCAGAAACCTCCAGAGGAATCTGTTTGCTTCC TGATTAGATCCAGTCAATGTTTTAAAGGTATTGTCAGAGAAAAACAGAGGGTCTGTACTAGCCATGCAAG GAGTCGCTCTAGCTGGTACCCGTAAAAGTTGTGGGAATTGTGACCCCCATCCCAAGGGGATGCCAAAATT TCTCTCATTCTTTTGGTATAAACTTAACATTAGCCAGGGAGGTTCTGGCTAACGTTAAATGCTGCTATAC AACTGCTTTGCAACAGTTGCTGGTATATTTAAATCATTAAATTTCAGCATTTACTAATACTGCAAAAAAA AAAAAAAAAAA SEQ ID NO: 18 - Homo sapiens FBJ murine osteosarcoma viral oncogene homolog (FOS), mRNA ATTCATAAAACGCTTGTTATAAAAGCAGTGGCTGCGGCGCCTCGTACTCCAACCGCATCTGCAGCGAGCA TCTGAGAAGCCAAGACTGAGCCGGCGGCCGCGGCGCAGCGAACGAGCAGTGACCGTGCTCCTACCCAGCT CTGCTCCACAGCGCCCACCTGTCTCCGCCCCTCGGCCCCTCGCCCGGCTTTGCCTAACCGCCACGATGAT GTTCTCGGGCTTCAACGCAGACTACGAGGCGTCATCCTCCCGCTGCAGCAGCGCGTCCCCGGCCGGGGAT AGCCTCTCTTACTACCACTCACCCGCAGACTCCTTCTCCAGCATGGGCTCGCCTGTCAA CGC GCAGGACT TCT GCACGGACCTG GCCGTCTCCAGTGCCAACTTCATTCCCACGGTCACTGCCATCTCGACCAGTCCGGA CCTGCAGTGGCTGGTGCAGCCCGCCCTCGTCTCCTCCGTGGCCCCATCGCAGACCAGAGCCCCTCACCCT TTCGGAGTCCCCGCCCCCTCCGCTGGGGCTTACTCCAGGGCTGGCGTTGTGAAGACCATGACAGGAGGCC GAGCGCAGAGCATTGGCAGGAGGGGCAAGGTGGAACAGTTATCTCCAGAAGAAGAAGAGAAAAGGAGAAT CCGAAGGGAAAGGAATAAGATGGCTGCAGCCAAATGCCGCAACCGGAGGAGGGAGCTGACTGATACACTC CAAGCGGAGACAGACCAACTAGAAGATGAGAAGTCTGCTTTGCAGACCGAGATTGCCAACCTGCTGAAGG AGAAGGAAAAACTAGAGTTCATCCTGGCAGCTCACCGACCTGCCTGCAAGATCCCTGATGACCTGGGCTT CCCAGAAGAGATGTCTGTGGCTTCCCTTGATCTGACTGGGGGCCTGCCAGAGGTTGCCACCCCGGAGTCT GAGGAGGCCTTCACCCTGCCTCTCCTCAATGACCCTGAGCCCAAGCCCTCAGTGGAACCTGTCAAGAGCA TCAGCAGCATGGAGCTGAAGACCGAGCCCTTTGATGACTTCCTGTTCCCAGCATCATCCAGGCCCAGTGG CTCTGAGACAGCCCGCTCCGTGCCAGACATGGACCTATCTGGGTCCTTCTATGCAGCAGACTGGGAGCCT CTGCACAGTGGCTCCCTGGGGATGGGGCCCATGGCCACAGAGCTGGAGCCCCTGTGCACTCCGGTGGTCA CCTGTACTCCCAGCTGCACTGCTTACACGTCTTCCTTCGTCTTCACCTACCCCGAGGCTGACTCCTTCCC CAGCTGTGCAGCTGCCCACCGCAAGGGCAGCAGCAGCAATGAGCCTTCCTCTGACTCGCTCAGCTCACCC ACGCTGCTGGCCCTGTGAGGGGGCAGGGAAGGGGAGGCAGCCGGCACCCACAAGTGCCACTGCCCGAGCT GGTGCATTACAGAGAGGAGAAACACATCTTCCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTG TGCGTGAAACACACCAGGCTGTGGGCCTCAAGGACTTGAAAGCATCCATGTGTGGACTCAAGTCCTTACC TCTTCCGGAGATGTAGCAAAACGCATGGAGTGTGTATTGTTCCCAGTGACACTTCAGAGAGCTGGTAGTT AGTAGCATGTTGAGCCAGGCCTGGGTCTGTGTCTCTTTTCTCTTTCTCCTTAGTCTTCTCATAGCATTAA CTAATCTATTGGGTTCATTATTGGAATTAACCTGGTGCTGGATATTTTCAAATTGTATCTAGTGCAGCTG ATTTTAACAATAACTACTGTGTTCCTGGCAATAGTGTGTTCTGATTAGAAATGACCAATATTATACTAAG AAAAGATACGACTTTATTTTCTGGTAGATAGAAATAAATAGCTATATCCATGTACTGTAGTTTTTCTTCA ACATCAATGTTCATTGTAATGTTACTGATCATGCATTGTTGAGGTGGTCTGAATGTTCTGACATTAACAG TTTTCCATGAAAACGTTTTATTGTGTTTTTAATTTATTTATTAAGATGGATTCTCAGATATTTATATTTT TATTTTATTTTTTTCTACCTTGAGGTCTTTTGACATGTGGAAAGTGAATTTGAATGAAAAATTTAAGCAT TGTTTGCTTATTGTTCCAAGACATTGTCAATAAAAGCATTTAAGTTGAATGCGACCAA SEQ ID NO: 19 - Homo sapiens FOS-like antigen 1 (FOSL1), mRNA ACGGGCCAAGGCGGCGCGTCTCGGGGGTGGAGCCTGGAGGTGACCGCGCCGCTGCAACGCCCCCACCCCC CGCGGTCGCAGTGGTTCAGCCCGAGAACTTTTCATTCATAAAAAGAAAAGACTCCGCACGGCGCGGGTGA GTCAGAACCCAGCAGCCGTGTACCCCGCAGAGCCGCCAGCCCCGGGCATGTTCCGAGACTTCGGGGAACC CGGCCCGAGCTCCGGGAACGGCGGCGGGTACGGCGGCCCCGCGCAGCCCCCGGCCGCAGCGCAGGCAGCC CAGCAGAAGTTCCACCTGGTGCCAAGCATCAACACCATGAGTGGCAGTCAGGAGCTGCAGTGGATGGTAC AGCCTCATTTCCTGGGGCCCAGCAGTTACCCCAGGCCTCTGACCTACCCTCAGTACAGCCCCCCACAACC CCGGCCAGGAGTCATCCGGGCCCTGGGGCCGCCTCCAGGGGTACGTCGAAGGCCTTGTGAACAGATCAGC CCGGAGGAAGAGGAGCGCCGCCGAGTAAGGCGCGAGCGGAACAAGCTGGCTGCGGCCAAGTGCAGGAACC GGAGGAAGGAACTGACCGACTTCCTGCAGGCGGAGACTGACAAACTGGAAGATGAGAAATCTGGGCTGCA GCGAGAGATTGAGGAGCTGCAGAAGCAGAAGGAGCGCCTAGAGCTGGTGCTGGAAGCCCACCGACCCATC TGCAAAATCCCGGAAGGAGCCAAGGAGGGGGACACAGGCAGTACCAGTGGCACCAGCAGCCCACCAGCCC CCTGCCGCCCTGTACCTTGTATCTCCCTTTCCCCAGGGCCTGTGCTTGAACCTGAGGCACTGCACACCCC CACACTCATGACCACACCCTCCCTAACTCCTTTCACCCCCAGCCTGGTCTTCACCTACCCCAGCACTCCT GAGCCTTGTGCCTCAGCTCATCGCAAGAGTAGCAGCAGCAGCGGAGACCCATCCTCTGACCCCCTTGGCT CTCCAACCCTCCTCGCTTTGTGAGGCGCCTGAGCCCTACTCCCTGCAGATGCCACCCTAGCCAATGTCTC CTCCCCTTCCCCCACCGGTCCAGCTGGCCTGGACAGTATCCCACAT CCA ACTCCAGCAACTTCTTCTCCA T CCCTCTAATGAGACTGACCATATTGTGCTTCACAGTAGAGCCAGCTTGGGGCCACCAAAGCTGCCCACT GTTTCTCTTGAGCTGGCCTCTCTAGCACAATTTGCACTAAATCAGAGACAAAATATTTCCCATTTGTGCC AGAGGAATCCTGGCAGCCCAGAGACTTTGTAGATCCTTAGAGGTCCTCTGGAGCCCTAACCCCTTCCAGA TCACTGCCACACTCTCCATCACCCTCTTCCTGTGATCCACCCAACCCTATCTCCTGACAGAAGGTGCCAC TTTACCCACCTAGAACACTAACTCACCAGCCCCACTGCCAGCAGCAGCAGGTGATTGGACCAGGCCATTC TGCCGCCCCCTCCTGAACCGCACAGCTCAGGAGGCGCCCTTGGCTTCTGTGATGAGCTGATCTGCGGATC TCAGCTTTGAGAAGCCTTCAGCTCCAGGGAATCCAAGCCTCCACAGCGAGGGCAGCTGCTATTTATTTTC CTAAAGAGAGTATTTTTATACAAACCTACCAAAATGGAATAAAAGGCTTGAAGCTGTG SEQ ID NO: 20 - Homo sapiens forkhead box N3 (FOXN3), transcript variant 1, mRNA CGCGATCTGCTGCAGCTCGGCCGGGAGACGGCGCGACCCGGCGGCGGGGCCACCCGCGAGTCCAGCGTCG CCGCAGCCCCCCAATGCGGCCGCGAGAAGCAGCGGGGGGGCA GGC GATCGAAGGAGCCTTCACGTAA ATG GGTCCAGTCATGCCTCCCAGTAAGAAGCCAGAAAGCTCAGGAATTAGTGTCTCCAGTGGACTGAGTCAGT GTTACGGGGGCAGCGGTTTCTCCAAGGCCCTTCAGGAAGACGATGACCTCGACTTTTCTCTGCCTGACAT CCGATTAGAAGAGGGGGCCATGGAAGATGAAGAGCTGACCAACCTGAACTGGCTGCACGAGAGCAAGAAC TTGCTGAAGAGCTTTGGGGAGTCGGTCCTCAGGAGTGTCAGCCCCGTCCAGGACCTGGACGATGACACCC CCCCATCCCCTGCCCACTCTGACATGCCCTACGATGCCAGGCAGAACCCCAACTGCAAACCCCCCTACTC CTTCAGCTGCCTCATATTTATGGCCATCGAGGACTCTCCAACCAAGCGCCTGCCAGTGAAGGATATCTAC AACTGGATCTTGGAACATTTTCCGTATTTTGCAAATGCACCTACTGGGTGGAAAAACTCAGTGAGACACA ATTTATCATTGAATAAGTGTTTTAAGAAAGTGGACAAAGAGAGGAGTCAGAGTATTGGGAAAGGGTCGTT GTGGTGCATAGACCCAGAGTATAGACAAAATCTAATTCAGGCTTTGAAAAAGACACCTTATCACCCACAC CCACACGTGTTCAATACACCTCCCACCTGTCCTCAGGCATATCAAAGCACATCAGGTCCACCCATCTGGC CGGGCAGTACCTTCTTCAAGAGAAATGGAGCCCTTCTCCAAGATCCTGACATTGATGCTGCCAGTGCCAT GATGCTTTTGAATACTCCCCCTGAGATACAAGCAGGTTTTCCTCCAGGAGTGATCCAAAATGGAGCGCGG GTCCTGAGCCGAGGGCTGTTTCCTGGCGTGCGGCCGCTGCCAATCACTCCCATTGGGGTGACAGCGGCCA TGAGGAATGGCATCACCAGCTGCCGGATGCGGACTGAGAGTGAGCCATCTTGTGGCTCCCCAGTGGTCAG CGGAGACCCCAAGGAGGATCACAACTACAGCAGTGCCAAGTCCTCCAACGCCCGGAGCACCTCGCCCACC AGCGACTCCATCTCCTCCTCCTCCTCCTCAGCCGACGACCACTATGAGTTTGCCACCAAGGGGAGCCAGG AGGGCAGCGAGGGCAGCGAGGGGAGCTTCCGGAGCCACGAGAGCCCCAGCGACACGGAAGAGGACGACAG GAAGCACAGCCAGAAGGAGCCCAAGGATTCTCTGGGGGACAGCGGGTACGCATCCCAGCACAAGAAGCGC CAGCACTTCGCCAAGGCCAGGAAGGTCCCCAGCGACACACTGCCCCTCAAAAAGAGACGCACCGAAAAGC CCCCCGAGAGCGATGATGAGGAGATGAAAGAAGCGGCAGGGTCCCTCCTGCACTTAGCAGGGATCCGGTC CTGTTTGAATAACATCACCAATCGGACGGCAAAGGGGCAGAAAGAGCAAAAGGAAACCACAAAAAATTAA AAACAAGTCACTGATTTGTTTTGAACTTACGACCATTTGGTTTCAGCATGTCAGGAGATTTCTAATGATT TGTGGCAATATCAGCAATTTTTTTTCTTTTTTCTTGTTTTTGGTTTGGTTTTCTTTCTTTTCTTTTCCTT TTATTTTGTTTTAATTTGCCCCCTCTTCTTTGTTTTGGACCCTTAAGAATTTTATTTTTAAAGGAGATTG AAGCCATAGAACTCATATTGACACTCAGCTGTTTTACAAAAGCTTTTCATTATCTGAAGACAAAACCGAA AAAGCCAAAATTACCATTGCTTCCTCCAGCTTGTCAGAAACCTGTGGCTGAATCCGCAGGGATGTCAACG TCAATATCACAGGAACACACATTCGGCACCTAGAAGGCACGTGGGCAAAGTAATCATCGTTCAGGCCCAA CCCTTAGGTTTAAAAAGTCAGGTTGTCCATCCCATTGGGTTCACTGAGTGAAGGCACATAAAGCAATTGA GGAGGAGGAGGAACCCCTCGTCCCCCTAGGAGCAGACCCAAGCTTGTGGCACCAGGCATCTGATGGTGCC AGGAAAGCCACTGGAATTGTCACACGGCGAGCACAGAGGGCCGGCCACCAGTCCTCGATGCTTCTGAACC CTGAAGCCCGATGACATCTTACGAGGTGGACGTTGGACTGTTCATGCGCATCGGGTGTCAGTGACTCATG GAGAAGAAATGGGGTAAATTTTTAGTGATGTTGCTAATCATTGAATTCTGTTCTCTATTAAATTAAGAAA ATGTTCCAAAAGCCATAAGCCTGAAGATTGGCCCTGTGCACGCACGCACACACACACACACACACACACA CACACACACACACACACGAAGGAGAGAGAGAGAAAACTGATGGGGAAAACAAGCTGTGTCTTCTTAACTG CCCAAGTGAAAAGCAACCAAGTCCAGGAAATTACAATAGCTGTTAAGGAAAGGAAATAATGGTACAGATC TTTTTCTGTCTATCAAAACTATTTGATCCAAGTGAAAAAAAAAAAAAAACTAGAAAGCTACGGAACCTGC CATTAGTATTGTGGTGTATTTTTAAGATTAAAGGTACACTGATGGACAAAAAAAAAAAGTAAAACATGGC AAAAAATAAAATAACTCCTATACTGCCCTCAAAATGGAGTTTGCAATTAATATCAGGATTTATCTTTGCA AAAATCAGTGATTTCCACATTCAGCCAGTATAGCCAGCAGAAATTTCTGATCCACAATGCATGGATTCCT TTGAAGAAAAAAAAGAAAAAGAGAAAAAAATCACAAAAACAAACTTTTTTTATTCAAAAGTAACAAAGTT CTTGTAAGGTAAATAATGTATTTAGCATGAAGCATGAATTATTTTCATATAAATATAGAAAATAGAGAAA AGGCTATGCCTGTAATTTTTAAGCCCTTAGGCTTAGAGTTTCTTTTGGTTTTCTTCTTTTTTCTTTCCTT TTCTTTGCTTTCTTTTTTTCCTTTTTGTTTTTGTTTTTGTTTTTTGTTTTTGTTTTTTTTTCGGGTTATT TTGTTTTGGTTTTTTGAAGCAGGTGTTTAAGGTTTAACCTTCTTCAGGGACAAATTCTGACTGTTGGGGA ACTTACTCTGCAATATAAAAATATCTTCATGCTCTGGTAGGGCTTGGATGGTTGAACTCTGTACTGCCTT GTGTGCACTTCAGCCCCGACCCCCTCTGATTCTCTGTTGAAAAGTGTGTCCTTTCTCTCTGTCTGTACAT GTTTAACATGACGCAATAATTTGAGGGCAAACTTAGTAGTGAGTGTGTATGATAGAATCAAGAGAATTAT GGGACGCTTACTTGAGAAAATCATTACCATGATTTGGTTCTAGGAAAAAGGCAGTGAATAATTATGCAAA TTAGCCAGAAGAAGGGGAACCGTGCTAATGGGCCTTATTGGGTGAGGGGACGAGATGGGGTTCATGTGAA GGAGGAAGCGATGCCGAGGTAGGAAAGGCCAGCCCCAGACATCCTATCGCCACAATGCCATGTCGCAATA GGAAGCAGGGGCCGGCCATCGCTACCTTCAGCACACTGACCAACCTGGAATTAAGACCACCTAGATTGCG AGAGCTGAATTTAGAAACCAGACAACGTCATGCAGCCCAGAAACTCCTGTTGTTACCTTTGCCTAAGAAA TTTTCTTTAATGGCGGGGGCGGGGGGCGGGGGTACAAAGAGAAATCTCTAAAAGAATATGATCTTCCATC CAAGTGGAGGGAAACTTTAAAACAAAAACACCCAGTACTGTGGCTCAGGATATGATGCGTGAGGAGAGGG AGGGAACAGAGATGACCTTAACTTTTAAAAAAGGGACTGCTGTGGGCCAAAGCCAAGCCCATCTGCCAGG ACGAGGTAATGTCAGAGCTCCATCAGCCCGGACAGTGGGAACTAACTGGTGCATTCCCCACACTTACCTT CCGGTGGGTTGCTGATGAGAGAACCTGAAAAAACCTACACCTCTACAGCAGGTCGAATTCATGACCTGAA GCTGAATACTTCCAGCATATTTATTCAGGGTGTAGGTGGGAATAAAGTATCTTCGCAGTGCTCTGTTCCC TCCGTCTCCCCAGACATCTGACACCCTAAAAGCCATCCACAGCTATGGAACCTGAGCGACACCTTGATTT GTGTTGTCACCTGACCAAGCCTAAAGACCTCCAGCTCAGTCCCCCACCTTCATCCCACCCCACAGATGAT AAAATTCAGACCTCTCTCCTGAAAGGCAGAGGTTCAACATTCAGGACTGTTTCTGGCCGAGGACTTCTTC CAATTAAAACCCCCACCGTGGGCTGTCTCCCCTCATTTCATTTTTCTAAAGGGGCAGAGGCCTCTTTTAG AAAATAATAAAATGCAATGTGTGTGATTTACTTTTCTGATCTCTTTGAGAAATAGAGAAATATAAAAGTG TGTTCTTAACTCCAGAACCACTCTTTTTGCATAAATACCTCATCGGGCAGCTTTCTAAGTGTGATTTTCC TGAGTCTCCCTTCGTTGGATCTGCCGGAAGACTTGTCGGGGAACCTTTAGTGAGGGTACTTCTTCCTATT TTTCTTCTGTTTTTGGAGGCATACACATTATGCATAACCAAAACAATGGCTCAATTGTGTTTAACTTTGT ATTTTGATTGTTGAGAACAAAAACAAAAAGTATCAATGTGTATGTGGCTGTTTGTAGTGAATTTATTGGA GAATGAGGTTGTCCGTGTCCTTAACAAGCCAAGGGGCAGGAGGCACCCTCTCTTATCCCCTCCTCCAAGA GCAGTAGAGAATTTAAGCACAAGCCTATTTGTGAAAGAATATTTTGCTTAAGTGTCATTCACTTTAGTCT TGGAATTCCTTCCCAAACGTCAGGTGTTCTTTTAGCTTCCAAACTAGCATATGTATCCATTAGTCTGACA GATCGCCTGAACACCATTAAGAGGTGTGGCGTTTTTGCTTTCATTTCTCCTGCTGGGAGAAGTGGCGGTT CATGTGTCATTCCAGTATCTCACATACTCACACGGGGCAGGGGGGAGGGGGAAACGGGGAACTATAGCAA TATTTAAAGATGCTTTGGAAACCAACCGTGAACACATCAACACCACGACGTCTACGATTACTTGCTATTG GCCCTCGGATACATTTAAGAGAAAGAGACAGTCACTCTTTTTTTTCTTAAATGATATACATATAAACAGT TATTTTTATCCTATTATAATTGTCTTTTGTCTTTATCTAGTACTATGTGGAAAGGGTTTGCATCATAGAT TTTTCCCAGCCTTATAATATACCATAAGCTCCTACTTCCCTGCCCCTCCCTAATCAGTATTCTTTCAAGA GTTCTTTGGTGAAGCCATCTATCTGAAACTAAAATGAACCAAACCCATATTTCACTGGTGGTTGGAGAAA ACCATGGCCAAAACGATTGTGGCAGGTCTCAATCTTGGGAGTTTTTAAGAAGGAATGTGCCAGAGGCCGA TTCCCAAGAACAGAGTTTTCTTTTGTTTTGCAGAGGCATTCAATGTGTCTAGTGCTTGCTGGCCACAGCA GTTACTACCACAGAGCCTTCTGGGAGGGGCCGTTGTGTTGAAGGAGGCTCCTGCCTGAGGGACAGCATCA GGCAGTGGGCTCTGTAGAGTGAGAACCAGGTGGAGGCCTTCTCTGCCCAGCTCAGAGTTCTGCACCACGC CAGGACTGCCCAGGCCAAGGGCTACTGACGCAAGTTCCACTCATTCCACTCTGTGGGGGGCGCCTTGGGC CTCTCCTGGAAGGGCTCTTGGAGAAGGAATTGGAGTTACGTACAAGTGACCTAAATGGGAAGCTTTTCTA GATGAGATTGGATTAAATTCCATGTGATTTCTCTTTCCCTTTAATCCAGGTTGGGACTCGTTTCTTTCTG GTGGATCACAGCTGCCCAGATGTTGCAATTGATTTTTATGTTTCTGTAGAGAAGTATTTTTCTTTCATCT TCAGGATTTTTTTTGCCACCAAAAGAAAACATTGGAACTCTGTGTTTCCTCTTGATTGTGACTTCCCAGT GTTGACAGTTAAGTCCTTAGTGTCGTAGGTCCCAGCCCACCAATACTATATCAAACACTGTTATGCACAT AATGCAGCACTCTGATCTAATTTAAATAATACTTTTTTATTATTTATACTACTATATATAATATACATCA ACACTTTTGCTATATAACCTAAGTGATAACCCTCTTTTAGTTACCTGCCAAACTCTGGACTTGGTTTATA TTGCAGTTAACACAGTTACAAAGCTGTAATGGTGTCTTTTTTTCCTTTGTAACGGAATGTGTAAATCAAA GTATATACATTGTGTGGTGTTCCTGTTTCTGGAGTTTCATGAGGATTTACACATGGCATTCAGTGTTCTG TATAGATCTGCCTACCTTTGTGAATTCATCTGTTAACCCCTCTTCCTTTGAGAGAGCACCGGCGATGGTG GTTAACTCCTTGTGTTTTCTCTCTCTCCTACTGGTTATTCTTGAATTAAGCACAGACTCGTCAGCTCGGT TGCTTTATCATGAATAATGTGTGTGACCTTGCAGTTCTTCCACAGTTCAGCAAACAAGTGCTAGCTTCAC TGACCAAAAATTAAGGAAGGAAAACACAGTTTTTAAAACGATCCATCTTTTAACAGCCGAAACCGATGTG TCTATGGTGCTGCACCTTGCTGTTGTACTTCTGAAATCAGACGTGTGTGAACGATCATTTCTGACTTAAC CGTGAGATGCTCACGAGTACCCTTCCTGTTGTTTTGTTAGCATTGAAATCGAGACTATTTATTTGGAATA TATACAACAGTGTTTTTCCACTGTATTTCATTTGCAAAAGTTGAGAACTGCTTTCTCTACCTTTTGCAAA ATAATTGATATTCCATATTGGATTCTCAAAGACTTCGATATGCTGAACCTATTAAACCTAGAAATTGTAT TCATCCTTTCATGACTGTGGCCTGAGTTCCCCAGCCCCTCTCCTCCTTTTTTTTAGATGAGATTTAGCAC ACTCTCAGTTATTTAAACATGCAACATTTCTTGAGTATGTATGTTGAGGCCATCTGAGCTCATAGCTGAT TCAGTAACCAGTTTCATGCTGTGTCATTCACACTCACTACTTAATACTGCCATGGTGAAAATGTGGAGGA AAAATGTATCCATGTGTGTCTGGGAAGCATATACACTTGTACATTTTTTAATACTCTGATTCTGTAACAT TTCTGAGTTTTGTTTTGTTTTACAGAAAAAAAAAAAAAGTGATAAAGCAATCAGAAGACCAAGAGGTTTA CTATTGATGCTTAGGGTCGTCTGACCTTGGCTGGCCAATAGACCTACACGGCCAAATTAATTTACGAGAG TAATAATTTTTCAAAAGCCAATTTTTTTTCTGTATTTTCTGTATGAAACTGCCAATATCATGAATAGAAA GGGAGAACCATAAAGGAGAAAGAACGTGATGTTCTGTTATGTTCATGTAAACCTAAAGAAACAGTGTGGA GGCAGGCGCGATCAGCCGAACTCTAGGGACTTGGTGTTGCTTGGAAGGCATCCATACCTGCATTTTGCAT TCTTCGTATGTAATCATATTGCCAAAGACAAACTATTTCATCATTTATTGTAAATAACACTTTTCCCCAG ACCTACCATAAAGTTTCTGTGATGTATTGTCTTCCAGTTGCAATAAAAATTACTGAGTTGCATCAATTGA AGAAAAACACCAAAAA SEQ ID NO: 21 - Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mRNA AAATTGAGCCCGCAGCCTCCCGCTTCGCTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTG CGTCGCCAGCCGAGCCACATCGCTCAGACACCATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCG TATT GGG CGCCTGGTCACCAGGGCTGCTT TTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCC TTCATTGACCTCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCACCG TCAAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGGAGCGAGATCCCTC CAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGCGTCTTCACCACCATGGAG AAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGGTCATCATCTCTGCCCCCTCTGCTGATGCCCCCA TGTTCGTCATGGGTGTGAACCATGAGAAGTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCAC CACCAACTGCTTAGCACCCCTGGCCAAGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACC ACAGTCCATGCCATCACTGCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCC GCGGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTCATCCCTGA GCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTGTCAGTGGTGGACCTGACC TGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAGGTGGTGAAGCAGGCGTCGGAGGGCCCCC TCAAGGGCATCCTGGGCTACACTGAGCACCAGGTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTC CACCTTTGACGCTGGGGCTGGCATTGCCCTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAAC GAATTTGGCTACAGCAACAGGGTGGTGGACCTCATGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGA CCACCAGCCCCAGCAAGAGCACAAGAGGAAGAGAGAGACCCTCACTGCTGGGGAGTCCCTGCCACACTCA GTCCCCCACCACACTGAATCTCCCCTCCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTA GGGAGCCGCACCTTGTCATGTACCATCAATAAAGTACCCTGTGCTCAACC SEQ ID NO: 22 - Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mRNA AAATTGAGCCCGCAGCCTCCCGCTTCGCTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTG CGTCGCCAGCCGAGCCACATCGCTCAGACACCATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCG TATT GGG CGCCTGGTCACCAGGGCTGCTT TTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCC TTCATTGACCTCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCACCG TCAAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGGAGCGAGATCCCTC CAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGCGTCTTCACCACCATGGAG AAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGGTCATCATCTCTGCCCCCTCTGCTGATGCCCCCA TGTTCGTCATGGGTGTGAACCATGAGAAGTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCAC CACCAACTGCTTAGCACCCCTGGCCAAGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACC ACAGTCCATGCCATCACTGCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCC GCGGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTCATCCCTGA GCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTGTCAGTGGTGGACCTGACC TGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAGGTGGTGAAGCAGGCGTCGGAGGGCCCCC TCAAGGGCATCCTGGGCTACACTGAGCACCAGGTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTC CACCTTTGACGCTGGGGCTGGCATTGCCCTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAAC GAATTTGGCTACAGCAACAGGGTGGTGGACCTCATGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGA CCACCAGCCCCAGCAAGAGCACAAGAGGAAGAGAGAGACCCTCACTGCTGGGGAGTCCCTGCCACACTCA GTCCCCCACCACACTGAATCTCCCCTCCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTA GGGAGCCGCACCTTGTCATGTACCATCAATAAAGTACCCTGTGCTCAACC SEQ ID NO: 23 - Homo sapiens GATA binding protein 3 (GATA3), transcript variant 1, mRNA GGCGCCGTCTTGATACTTTCAGAAAGAATGCATTCCCTGTAAAAAAAAAAAAAAAATACTGAGAGAGGGA GAGAGAGAGAGAAGAAGAGAGAGAGACGGAGGGAGAGCGAGACAGAGCGAGCAACGCAATCTGACCGAGC AGGTCGTACGCCGCCGCCTCCTCCTCCTCTCTGCTCTTCGCTACCCAGGTGACCCGAGGAGGGACTCCGC CTCCGAGCGGCTGAGGACCCCGGTGCAGAGGAGCCTGGCTCGCAGAATTGCAGAGTCGTCGCCCCTTTTT ACAACCTGGTCCCGTTTTATTCTGCCGTACCCAGTTTTTGGATTTTTGTCTTCCCCTTCTTCTCTTTGCT AAACGACCCCTCCAAGATAATTTTTAAAAAACCTTCTCCTTTGCTCACCTTTGCTTCCCAGCCTTCCCAT CCCCCCACCGAAAGCAAATCATTCAACGACCCCCGACCCTCCGACGGCAGGAGCCCCCCGACCTCCCAGG CGGACCGCCCTCCCTCCCCGCGCGCGGGTTCCGGGCCCGGCGAGAGGGCGCGAGCACAGCCGAGGCCATG GAGGTGACGGCGGACCAGCCGCGCTGGGTGAGCCACCACCACCCCGCCGTGCTCAACGGGCAGCACCCGG ACACGCACCACCCGGGCCTCAGCCACTCCTACATGGACGCGGCGCAGTACCCGCTGCCGGAGGAGGTGGA TGTGCTTTTTAACATCGACGGTCAAGGCAACCACGTCCCGCCCTACTACGGAAACTCGGTCAGGGCCACG GTGCAGAGGTACCCTCCGA CCC ACCACGGGAGCCAGGTGTGCCG CCCGCCTCTGCTTCATGGATCCCTAC CCTGGCTGGACGGCGGCAAAGCCCTGGGCAGCCACCACACCGCCTCCCCCTGGAATCTCAGCCCCTTCTC CAAGACGTCCATCCACCACGGCTCCCCGGGGCCCCTCTCCGTCTACCCCCCGGCCTCGTCCTCCTCCTTG TCGGGGGGCCACGCCAGCCCGCACCTCTTCACCTTCCCGCCCACCCCGCCGAAGGACGTCTCCCCGGACC CATCGCTGTCCACCCCAGGCTCGGCCGGCTCGGCCCGGCAGGACGAGAAAGAGTGCCTCAAGTACCAGGT GCCCCTGCCCGACAGCATGAAGCTGGAGTCGTCCCACTCCCGTGGCAGCATGACCGCCCTGGGTGGAGCC TCCTCGTCGACCCACCACCCCATCACCACCTACCCGCCCTACGTGCCCGAGTACAGCTCCGGACTCTTCC CCCCCAGCAGCCTGCTGGGCGGCTCCCCCACCGGCTTCGGATGCAAGTCCAGGCCCAAGGCCCGGTCCAG CACAGAAGGCAGGGAGTGTGTGAACTGTGGGGCAACCTCGACCCCACTGTGGCGGCGAGATGGCACGGGA CACTACCTGTGCAACGCCTGCGGGCTCTATCACAAAATGAACGGACAGAACCGGCCCCTCATTAAGCCCA AGCGAAGGCTGTCTGCAGCCAGGAGAGCAGGGACGTCCTGTGCGAACTGTCAGACCACCACAACCACACT CTGGAGGAGGAATGCCAATGGGGACCCTGTCTGCAATGCCTGTGGGCTCTACTACAAGCTTCACAATATT AACAGACCCCTGACTATGAAGAAGGAAGGCATCCAGACCAGAAACCGAAAAATGTCTAGCAAATCCAAAA AGTGCAAAAAAGTGCATGACTCACTGGAGGACTTCCCCAAGAACAGCTCGTTTAACCCGGCCGCCCTCTC CAGACACATGTCCTCCCTGAGCCACATCTCGCCCTTCAGCCACTCCAGCCACATGCTGACCACGCCCACG CCGATGCACCCGCCATCCAGCCTGTCCTTTGGACCACACCACCCCTCCAGCATGGTCACCGCCATGGGTT AGAGCCCTGCTCGATGCTCACAGGGCCCCCAGCGAGAGTCCCTGCAGTCCCTTTCGACTTGCATTTTTGC AGGAGCAGTATCATGAAGCCTAAACGCGATGGATATATGTTTTTGAAGGCAGAAAGCAAAATTATGTTTG CCACTTTGCAAAGGAGCTCACTGTGGTGTCTGTGTTCCAACCACTGAATCTGGACCCCATCTGTGAATAA GCCATTCTGACTCATATCCCCTATTTAACAGGGTCTCTAGTGCTGTGAAAAAAAAAATGCTGAACATTGC ATATAACTTATATTGTAAGAAATACTGTACAATGACTTTATTGCATCTGGGTAGCTGTAAGGCATGAAGG ATGCCAAGAAGTTTAAGGAATATGGGAGAAATAGTGTGGAAATTAAGAAGAAACTAGGTCTGATATTCAA ATGGACAAACTGCCAGTTTTGTTTCCTTTCACTGGCCACAGTTGTTTGATGCATTAAAAGAAAATAAAAA AAAGAAAAAAGAGAAAAGAAAAAAAAAGAAAAAAGTTGTAGGCGAATCATTTGTTCAAAGCTGTTGGCCT CTGCAAAGGAAATACCAGTTCTGGGCAATCAGTGTTACCGTTCACCAGTTGCCGTTGAGGGTTTCAGAGA GCCTTTTTCTAGGCCTACATGCTTTGTGAACAAGTCCCTGTAATTGTTGTTTGTATGTATAATTCAAAGC ACCAAAATAAGAAAAGATGTAGATTTATTTCATCATATTATACAGACCGAACTGTTGTATAAATTTATTT ACTGCTAGTCTTAAGAACTGCTTTCTTTCGTTTGTTTGTTTCAATATTTTCCTTCTCTCTCAATTTTTGG TTGAATAAACTAGATTACATTCAGTTGGCCTAAGGTGGTTGTGCTCGGAGGGTTTCTTGTTTCTTTTCCA TTTTGTTTTTGGATGATATTTATTAAATAGCTTCTAAGAGTCCGGCGGCATCTGTCTTGTCCCTATTCCT GCAGCCTGTGCTGAGGGTAGCAGTGTATGAGCTACCAGCGTGCATGTCAGCGACCCTGGCCCGACAGGCC ACGTCCTGCAATCGGCCCGGCTGCCTCTTCGCCCTGTCGTGTTCTGTGTTAGTGATCACTGCCTTTAATA CAGTCTGTTGGAATAATATTATAAGCATAATAATAAAGTGAAAATATTTTAAAACTACAA SEQ ID NO: 24 - Homo sapiens guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1, mRNA CCGGGGACGGCTGCTGGAGCGGCGCCCGCCGCGGCTCAGCGCATTCCCGCTCTCCGCTTCCCTCTCCGCT GCGTCCCCGCGCGAAGATGGCAACCGAGGGGCTGCACGAGAACGAGACGCTGGCGTCGCTGAAGAGCGAG GCCGAGAGCCTCAAGGGCAAGCTGGAGGAGGAGCGAGCCAAGCTG CAC GATGTGGAGCTGCACCAGGTGG CGGAGCGGGTGGAGGCCCTGGGGCAGTTTGTCATGAAGACCAGAAGGACCCTCAAAGGCCACGGGAACAA AGTCCTGTGCATGGACTGGTGCAAAGATAAGAGGAGGATCGTGAGCTCGTCACAGGATGGGAAGGTGATC GTGTGGGATTCCTTCACCACAAACAAGGAGCACGCGGTCACCATGCCCTGCACGTGGGTGATGGCATGTG CTTATGCCCCATCGGGATGTGCCAT TGC TTGTGGTGGTTTGGATAATAAG TGTTCTGTGTACCCCTTGAC GTTTGACAAAAATGAAAACATGGCTGCCAAAAAGAAGTCTGTTGCTATGCACACCAACTACCTGTCGGCC TGCAGCTTCACCAACTCTGACATGCAGATCCTGACAGCGAGCGGCGATGGCACATGTGCCCTGTGGGACG TGGAGAGCGGGCAGCTGCTGCAGAGCTTCCACGGACATGGGGCTGACGTCCTCTGCTTGGACCTGGCCCC CTCAGAAACTGGAAACACCTTCGTGTCTGGGGGATGTGACAAGAAAGCCATGGTGTGGGACATGCGCTCC GGCCAGTGCGTGCAGGCCTTTGAAACACATGAATCTGACATCAACAGTGTCCGGTACTACCCCAGTGGAG ATGCCTTTGCTTCAGGGTCAGATGACGCTACGTGTCGCCTCTATGACCTGCGGGCAGATAGGGAGGTTGC CATCTATTCCAAAGAAAGCATCATATTTGGAGCATCCAGCGTGGACTTCTCCCTCAGTGGTCGCCTGCTG TTTGCTGGATACAATGATTACACTATCAACGTCTGGGATGTTCTCAAAGGGTCCCGGGTCTCCATCCTGT TTGGACATGAAAACCGCGTTAGCACTCTACGAGTTTCCCCCGATGGGACTGCTTTCTGCTCTGGATCATG GGATCATACCCTCAGAGTCTGGGCCTAATCATCTTCTGACAGTGCACTCATGTATACCTGAGAATTTGAA ATCTTCACATGTAAATAGATATTACTTCTAGAGGAGCTTAGAGTTTATTGCAGTGTAGCTTAGGGGAGCA ACCCATGGCTCACAGGTCACTAAGCGTCTCCAATATGACTATTAAAACTGTCACCTCTGGAAATACACTA GTGTGAGCCTTCAGCACTGCGAGAATACCTTCAAGTACAGTATTTTTCTTTTGGAACACTTTTTAAAATG TATCTGTTTTTAAGGTTATTCTAAATTATAGTAGCCTCAACTCATTCTGTCACCAGTAGAATTCAGCAGT TAATATATTCCATATTATTTCTTTGAATCAATTCATTTTCAGAGCACTTTAAAGTCTGATATTTCTCGAT GTGCACTGTGATGCCTGGAACCTTCCTCTGGAAGTGCTGATTTTATGGACTGAGGACTGGTGACTGGTCT GTGATAGAAGCAAATTCCAATTCCAAATGTAATTAGACAAAAATCATTTTTTTAGAATGTGTTTTTATTG TAAAAGTATCTTTTTCAGCTTCCTGTTCTATTGTCTTTTTTCAGATACAACATTTTTGTCTATGGTGAAC TGCTGTAAATGACGCAGAGAAATGCCTAAAAAGGACAGGTGGTTTGACTCATGGATGATGATGATGTCAC TGTGCCACTTGGACAGGGCGTTTTCTCTGAATTGAAGGGAAAGCCAATGGTGTTTGTAAACAAATGCTTC TGAGAGCAAAGAAAAGTCTTCTGTGTGGGAACACAAGATAGTAAACTTATTTAAAAACCTATTAGTAGAA TTAGTGGAAACACTTAGGTTAAAGTGAATCTTGTCCATATAAATTATATTCATGGCCGGGCGCGGTGGCT CACGCTTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGTTCGAGACCACG GTGAAACCCTGTCTCTACTAAAAAATACAAAAAATTAGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAG CTACTCGGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGCTTGCAGTGAGCCGAGGTCG AGCCACTGCAGCCTGGGTGACAAAGCGAGACTCCGTCTCAAAAAAAAAAAAAAATTATATTCATATGTAT TGCATTGCAATTATAATTACATATGCAGATTGATTGATAGTCATGAATAATAACGTCTGCTCCTCTTACA TAGAAAAACGATATTAAAAGAAGATCTTCTCTTTATTTGAGACTCAGAATTCCTTCTAGAAGAAGGAAGT GCTTTTTGTTATAGGATCCCTTCTTTTCCTTTTTTTGTTTTTTTGTAAGATGTAGATGCTTATTCTTTGC TTTAGAAAACTTCTCACTTAAAAAGATGGCATGCACCTAGGGGAATAAAAGGTCACCTCAGACACCAGGT GTCATTCCTGGTGAGGCCTGCCTCGTCGGTGGCCTGGGGTCTGCCGGCAGGTTCTGGCTGCACCTGAAGG CTGCGTGCACCTTGTCCCCTGGACAGGTCTCCTTTCCTGGCCCTGCTCCAGCCCAGCCCTTCTTCTAGTG GTAGCTCTGGCTTTGCAGGCCCAGCTCCAGGCCCTGCTCCTCAGAGAGACTCTTCCAGAGCTGGAGCTGG GCACAGCCATAAGACAGGACTGGACCAGATGCTCCTGTAAACATCCAGGGGTGTGCCAGGCCCACCCTCA CAACTGCTTGTTCAGGTATCGTGATGGGCCACTCGGTCCAAAATCAGCCAGGCCATCTTTTCCATCATCT CACTTCAAATAAACATAATAATTATATTTGATCATTTGC SEQ ID NO: 25 - Homo sapiens glutathione S-transferase mu 4 (GSTM4), transcript variant 2, mRNA AAGCTGGCGAGGCCGAGCCCCTCCTAGTGCTTCCGGACCTTGCTCCCTGAACACTCGGAGGTGGCGGTGG ATCTTACTCCTTCCAGCCAGTGAGGATCCAGCAACCTGCTCCGTGCCTCCCGCGCCTGTTGGTTGGAAGT GACGACCTTGAAGATCGGCCGGTTGGAAGTGACGACCTTGAAGATCGGCGGGCGCAGCGGGGCCGAGGGG GCGGGTCTGGCGCTAGGTCCAGCCCCTGCGTGCCGGGAACCCCAGAGGAGGTCGCAGTTCAGCCCAGCTG AGGCCTGTCTGCAGAATCGACACCAACCAGCATCATGTCCATGACACTGGGGTACTGGGACATCCGCGGG CTGGCCCACGCCATCCGCCTGCTCCTGGAATACACAGACTCAAGCTACGAGGAAAAGAAGTATACGATGG GGGACGCTCCTGACTATGACAGAAGCCAGTGGCTGAATGAAAAATTCAAGCTGGGCCTGGACTTTCCCAA TCTGCCCTACTTGATTGATGGGGCTCACAAGATCACCCAGAGCAACGCCATCCTGTGCTACATTGCCCGC AAGCACAACCTCTGTGGGGAGACAGAAGAGGAGAAGATTCGTCTGGACATTTTGGAGAACCAGGCTATGG ACGTCTCCAATCAGCTGGCCAGAGTCTGCTA CAG CCCTGACTTTGAGAAACTGAAG CCAGAATACTTGGA GGAACTTCCTACAATGATGCAGCACTTCTCACAGTTCCTGGGGAAGAGGCCATGGTTTGTTGGAGACAAG ATCACCTTTGTAGATTTCCTCGCCTATGATGTCCTTGACCTCCACCGTATATTTGAGCCCAACTGCTTGG ACGCCTTTCCAAATCTGAAGGACTTCATCTCCCGCTTTGAGGTTTCCTGTGGCATAATGTGATGGTCAAT TTTCTGCATCAACTTGACTGGGCTAAGGGATGCTCAGATGGCAGGTAAAATCATTGTGCTTGTGAGGGTG TTTCCAGAAGAGATTTGCCTTTGAATCAGAAGACAGCAAAGATTTCCTTCAGCAATGAAGGAGGCATCCA CCAAACTGTCAGGGCCCAGAGAGAAGAAAAAGACAGGAAGGGTGAATTTGACCTCTCTGACTGGGACATC CATCTCTGCCTATCCTGGGACCTCCACACTCCTGGTTCTCTGGCCTTCAGACTTGATCAGGGACTAACAC CATCGCCTCCCACCCCCACCTTTGTTCTGAGGCCTTTAGCCTCTGAATGATACCACTGGCTTTCCTGCTT CTCTATCCTGCAGTCGGCAGATCATGGGACTTCTTCACTCCAAAATTGTGTGAGCCAATTCCCATAACAG ATAGATAAATTTATAAATAAACACACAAATTTCCTACAGCCT SEQ ID NO: 26 - Homo sapiens major histocompatibility complex, class II, DR alpha (HLA-DRA), mRNA TTTTAATGGTCAGACTCTATTACACCCCACATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCC CGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATGGCCATAAGTGGAGTCCCTGTGCTAGGAT TTTTCATCATAGCTGTGCTGATGAGCGCTCAGGAATCA TGG GCTATCAAAGAAGAACATGTGA TCATCCA GGCCGAGTTCTATCTGAATCCTGACCAATCAGGCGAGTTTATGTTTGACTTTGATGGTGATGAGATTTTC CATGTGGATATGGCAAAGAAGGAGACGGTCTGGCGGCTTGAAGAATTTGGACGATTTGCCAGCTTTGAGG CTCAAGGTGCATTGGCCAACATAGCTGTGGACAAAGCCAACCTGGAAATCATGACAAAGCGCTCCAACTA TACTCCGATCACCAATGTACCTCCAGAGGTAACTGTGCTCACAAACAGCCCTGTGGAACTGAGAGAGCCC AACGTCCTCATCTGTTTCATAGACAAGTTCACCCCACCAGTGGTCAATGTCACGTGGCTTCGAAATGGAA AACCTGTCACCACAGGAGTGTCAGAGACAGTCTTCCTGCCCAGGGAAGACCACCTTTTCCGCAAGTTCCA CTATCTCCCCTTCCTGCCCTCAACTGAGGACGTTTACGACTGCAGGGTGGAGCACTGGGGCTTGGATGAG CCTCTTCTCAAGCACTGGGAGTTTGATGCTCCAAGCCCTCTCCCAGAGACTACAGAGAACGTGGTGTGTG CCCTGGGCCTGACTGTGGGTCTGGTGGGCATCATTATTGGGACCATCTTCATCATCAAGGGATTGCGCAA AAGCAATGCAGCAGAACGCAGGGGGCCTCTGTAAGGCACATGGAGGTGATGGTGTTTCTTAGAGAGAAGA TCACTGAAGAAACTTCTGCTTTAATGGCTTTACAAAGCTGGCAATATTACAATCCTTGACCTCAGTGAAA GCAGTCATCTTCAGCATTTTCCAGCCCTATAGCCACCCCAAGTGTGGATATGCCTCTTCGATTGCTCCGT ACTCTAACATCTAGCTGGCTTCCCTGTCTATTGCCTTTTCCTGTATCTATTTTCCTCTATTTCCTATCAT TTTATTATCACCATGCAATGCCTCTGGAATAAAACATACAGGAGTCTGTCTCTGCTATGGAATGCCCCAT GGGGCATCTCTTGTGTACTTATTGTTTAAGGTTTCCTCAAACTGTGATTTTTCTGAACACAATAAACTAT TTTGATGATCTTGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 27 - Homo sapiens v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 3, mRNA TGCCCTGCGCCCGCAACCCGAGCCGCACCCGCCGCGGACGGAGCCCATGCGCGGGGCGAACCGCGCGCCC CCGCCCCCGCCCCGCCCCGGCCTCGGCCCCGGCCCTGGCCCCGGGGGCAGTCGCGCCTGTGAACGGTGGG GCAGGAGACCCTGTAGGAGGACCCCGGGCCGCAGGCCCCTGAGGAGCGATGACGGAATATAAGCTGGTGG TGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACCATCCAGCTGATCCAGAACCATTTTGTGGACGA ATACGACCCCACTATAGAGGATTCCTACCGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGAC ATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCT TCCTGTGTGTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAGATCAA ACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGTGACCTGGCTGCACGCACT GTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCCTACATCGAGACCTCGGCC AAGA CCC GGCAGGGAGTGGAGGATG CCTTCTACACGTTGGTGCGTGAGATCCGGCAGCACAAGCTGCGGAAGCT GAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGAGCTGCAAGTGTGTGCTCTCCTGACGCAGGTGAGGG GGACTCCCAGGGCGGCCGCCACGCCCACCGGATGACCCCGGCTCCCCGCCCCTGCCGGTCTCCTGGCCTG CGGTCAGCAGCCTCCCTTGTGCCCCGCCCAGCACAAGCTCAGGACATGGAGGTGCCGGATGCAGGAAGGA GGTGCAGACGGAAGGAGGAGGAAGGAAGGACGGAAGCAAGGAAGGAAGGAAGGGCTGCTGGAGCCCAGTC ACCCCGGGACCGTGGGCCGAGGTGACTGCAGACCCTCCCAGGGAGGCTGTGCACAGACTGTCTTGAACAT CCCAAATGCCACCGGAACCCCAGCCCTTAGCTCCCCTCCCAGGCCTCTGTGGGCCCTTGTCGGGCACAGA TGGGATCACAGTAAATTATTGGATGGTCTTGAAAAAAAAAAAAAAAAAA SEQ ID NO: 28 - Homo sapiens interferon, alpha-inducible protein 27 (IFI27), transcript variant 1, mRNA GGGAACACATCCAAGCTTAAGACGGTGAGGTCAGCTTCACATTCTCAGGAACTCTCCTTCTTTGGGTCTG GCTGAAGTTGAGGATCTCTTACTCTCTAGGCCACGGAATTAACCCGAGCAGGCATGGAGGCCTCTGCTCT CACCTCATCAGCAGTGACCAGTGTGGCCAAAGTGGTCAGGGTGGCCTCTGGCTCTGCCGTAGTTTT GCC C CTG GCCAGGATTGCTACAGTT GTGATTGGAGGAGTTGTGGCCATGGCGGCTGTGCCCATGGTGCTCAGTG CCATGGGCTTCACTGCGGCGGGAATCGCCTCGTCCTCCATAGCAGCCAAGATGATGTCCGCGGCGGCCAT TGCCAATGGGGGTGGAGTTGCCTCGGGCAGCCTTGTGGCTACTCTGCAGTCACTGGGAGCAACTGGACTC TCCGGATTGACCAAGTTCATCCTGGGCTCCATTGGGTCTGCCATTGCGGCTGTCATTGCGAGGTTCTACT AGCTCCCTGCCCCTCGCCCTGCAGAGAAGAGAACCATGCCAGGGGAGAAGGCACCCAGCCATCCTGACCC AGCGAGGAGCCAACTATCCCAAATATACCTGGGGTGAAATATACCAAATTCTGCATCTCCAGAGGAAAAT AAGAAATAAAGATGAATTGTTGCAACTCTTCAAAA SEQ ID NO: 29 - Homo sapiens interleukin 11 receptor, alpha (IL11RA), transcript variant 3, mRNA AGAGGGCGAGGGCGAGGGCAGAGGGCGCTGGCGGCAGCGGCCGCGGAAGATGAGCAGCAGCTGCTCAGGG CTGAGCAGGGTCCTGGTGGCCGTGGCTACAGCCCTGGTGTCTGCCTCCTCCCCCTGCCCCCAGGCCTGGG GCCCCCCAGGGGTCCAGTATGGGCAGCCAGGCAGGTCCGTGAAGCTGTGTTGTCCTGGAGTGACTGCCGG GGACCCAGTGTCCTGGTTTCGGGATGGGGAGCCAAAGCTGCTCCAGGGACCTGACTCTGGGCTAGGGCAT GAACTGGTCCTGGCCCAGGCAGACAGCACTGATGAGGGCACCTACATCTGCCAGACCCTGGATGGTGCAC TTGGGGGCACAGTGACCCTGCAGCTGGGCTACCCTCCAGCCCGCCCTGTTGTCTCCTGCCAAGCAGCCGA CTATGAGAACTTCTCTTGCACTTGGAGTCCCAGCCAGATCAGCGGTTTACCCACCCGCTACCTCACCTCC TACAGGAAGAAGACAGTCCTAGGAGCTGATAGCCAGAGGAGGAGTCCATCCACAGGGCCCTGGCCATGCC CACAGGATCCCCTAGGGGCTGCCCGCTGTGTTGTCCACGGGGCTGAGTTCTGGAGCCAGTACCGGATTAA TGTGACTGAGGTGAACCCACTGGGTGCCAGCACACGCCTGCTGGATGTGAGCT TGC AGAGCATCTTGCGC CCT GACCC ACCCCAGGGCCTGCGGGTAGAGTCAGTACCAGGTTACCCCCGACGCCTGCGAGCCAGCTGGA CATACCCTGCCTCCTGGCCGTGCCAGCCCCACTTCCTGCTCAAGTTCCGTTTGCAGTACCGTCCGGCGCA GCATCCAGCCTGGTCCACGGTGGAGCCAGCTGGACTGGAGGAGGTGATCACAGATGCTGTGGCTGGGCTG CCCCATGCTGTACGAGTCAGTGCCCGGGACTTTCTAGATGCTGGCACCTGGAGCACCTGGAGCCCGGAGG CCTGGGGAACTCCGAGCACTGGGACCATACCAAAGGAGATACCAGCATGGGGCCAGCTACACACGCAGCC AGAGGTGGAGCCTCAGGTGGACAGCCCTCCTCCTCCAAGGCCCTCCCTCCAACCACACCCTCCCCTACTT GATCACAGGGACTCTGTGGAGCAGGTAGCTGTGCTGGCGTCTTTGGGAATCCTTTCTTTCCTGGGACTGG TGGCTGGGGCCCTGGCACTGGGGCTCTGGCTGAGGCTGAGACGGGGTGGGAAGGATGGATCCCCAAAGCC TGGGTTCTTGGCCTCAGTGATTCCAGTGGACAGGCGTCCAGGAGCTCCAAACCTGTAGAGGACCCAGGAG GGCTTCGGCAGATTCCACCTATAATTCTGTCTTGCTGGTGTGGATAGAAACCAGGCAGGACAGTAGATCC CTATGGTTGGATCTCAGCTGGAAGTTCTGTTTGGAGCCCATTTCTGTGAGACCCTGTATTTCAAATTTGC AGCTGAAAGGTGCTTGTACCTCTGATTTCACCCCAGAGTTGGAGTTCTGCTCAAGGAACGTGTGTAATGT GTACATCTGTGTCCATGTGTGACCATGTGTCTGTGAGGCAGGGAACATGTATTCTCTGCATGCATGTATG TAGGTGCCTGGGGAGTGTGTGTGGGTCCTTGGCTCTTGGCCTTTCCCCTTGCAGGGGTTGTGCAGGTGTG AATAAAGAGAATAAGGAAGTTCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA SEQ ID NO: 30 - Homo sapiens jun proto-oncogene (JUN), mRNA GACATCATGGGCTATTTTTAGGGGTTGACTGGTAGCAGATAAGTGTTGAGCTCGGGCTGGATAAGGGCTC AGAGTTGCACTGAGTGTGGCTGAAGCAGCGAGGCGGGAGTGGAGGTGCGCGGAGTCAGGCAGACAGACAG ACACAGCCAGCCAGCCAGGTCGGCAGTATAGTCCGAACTGCAAATCTTATTTTCTTTTCACCTTCTCTCT AACTGCCCAGAGCTAGCGCCTGTGGCTCCCGGGCTGGTGTTTCGGGAGTGTCCAGAGAGCCTGGTCTCCA GCCGCCCCCGGGAGGAGAGCCCTGCTGCCCAGGCGCTGTTGACAGCGGCGGAAAGCAGCGGTACCCACGC GCCCGCCGGGGGAAGTCGGCGAGCGGCTGCAGCAGCAAAGAACTTTCCCGGCTGGGAGGACCGGAGACAA GTGGCAGAGTCCCGGAGCGAACTTTTGCAAGCCTTTC CGT CGTCTTAGGCTTCTCCACGGCGGTA AAGAC CAGAAGGCGGCGGAGAGCCACGCAAGAGAAGAAGGACGTGCGCTCAGCTTCGCTCGCACCGGTTGTTGAA CTTGGGCGAGCGCGAGCCGCGGCTGCCGGGCGCCCCCTCCCCCTAGCAGCGGAGGAGGGGACAAGTCGTC GGAGTCCGGGCGGCCAAGACCCGCCGCCGGCCGGCCACTGCAGGGTCCGCACTGATCCGCTCCGCGGGGA GAGCCGCTGCTCTGGGAAGTGAGTTCGCCTGCGGACTCCGAGGAACCGCTGCGCCCGAAGAGCGCTCAGT GAGTGACCGCGACTTTTCAAAGCCGGGTAGCGCGCGCGAGTCGACAAGTAAGAGTGCGGGAGGCATCTTA ATTAACCCTGCGCTCCCTGGAGCGAGCTGGTGAGGAGGGCGCAGCGGGGACGACAGCCAGCGGGTGCGTG CGCTCTTAGAGAAACTTTCCCTGTCAAAGGCTCCGGGGGGCGCGGGTGTCCCCCGCTTGCCAGAGCCCTG TTGCGGCCCCGAAACTTGTGCGCGCAGCCCAAACTAACCTCACGTGAAGTGACGGACTGTTCTATGACTG CAAAGATGGAAACGACCTTCTATGACGATGCCCTCAACGCCTCGTTCCTCCCGTCCGAGAGCGGACCTTA TGGCTACAGTAACCCCAAGATCCTGAAACAGAGCATGACCCTGAACCTGGCCGACCCAGTGGGGAGCCTG AAGCCGCACCTCCGCGCCAAGAACTCGGACCTCCTCACCTCGCCCGACGTGGGGCTGCTCAAGCTGGCGT CGCCCGAGCTGGAGCGCCTGATAATCCAGTCCAGCAACGGGCACATCACCACCACGCCGACCCCCACCCA GTTCCTGTGCCCCAAGAACGTGACAGATGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGCGCGCCCTGGCC GAACTGCACAGCCAGAACACGCTGCCCAGCGTCACGTCGGCGGCGCAGCCGGTCAACGGGGCAGGCATGG TGGCTCCCGCGGTAGCCTCGGTGGCAGGGGGCAGCGGCAGCGGCGGCTTCAGCGCCAGCCTGCACAGCGA GCCGCCGGTCTACGCAAACCTCAGCAACTTCAACCCAGGCGCGCTGAGCAGCGGCGGCGGGGCGCCCTCC TACGGCGCGGCCGGCCTGGCCTTTCCCGCGCAACCCCAGCAGCAGCAGCAGCCGCCGCACCACCTGCCCC AGCAGATGCCCGTGCAGCACCCGCGGCTGCAGGCCCTGAAGGAGGAGCCTCAGACAGTGCCCGAGATGCC CGGCGAGACACCGCCCCTGTCCCCCATCGACATGGAGTCCCAGGAGCGGATCAAGGCGGAGAGGAAGCGC ATGAGGAACCGCATCGCTGCCTCCAAGTGCCGAAAAAGGAAGCTGGAGAGAATCGCCCGGCTGGAGGAAA AAGTGAAAACCTTGAAAGCTCAGAACTCGGAGCTGGCGTCCACGGCCAACATGCTCAGGGAACAGGTGGC ACAGCTTAAACAGAAAGTCATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGCAA ACATTTTGAAGAGAGACCGTCGGGGGCTGAGGGGCAACGAAGAAAAAAAATAACACAGAGAGACAGACTT GAGAACTTGACAAGTTGCGACGGAGAGAAAAAAGAAGTGTCCGAGAACTAAAGCCAAGGGTATCCAAGTT GGACTGGGTTGCGTCCTGACGGCGCCCCCAGTGTGCACGAGTGGGAAGGACTTGGCGCGCCCTCCCTTGG CGTGGAGCCAGGGAGCGGCCGCCTGCGGGCTGCCCCGCTTTGCGGACGGGCTGTCCCCGCGCGAACGGAA CGTTGGACTTTTCGTTAACATTGACCAAGAACTGCATGGACCTAACATTCGATCTCATTCAGTATTAAAG GGGGGAGGGGGAGGGGGTTACAAACTGCAATAGAGACTGTAGATTGCTTCTGTAGTACTCCTTAAGAACA CAAAGCGGGGGGAGGGTTGGGGAGGGGCGGCAGGAGGGAGGTTTGTGAGAGCGAGGCTGAGCCTACAGAT GAACTCTTTCTGGCCTGCCTTCGTTAACTGTGTATGTACATATATATATTTTTTAATTTGATGAAAGCTG ATTACTGTCAATAAACAGCTTCATGCCTTTGTAAGTTATTTCTTGTTTGTTTGTTTGGGTATCCTGCCCA GTGTTGTTTGTAAATAAGAGATTTGGAGCACTCTGAGTTTACCATTTGTAATAAAGTATATAATTTTTTT ATGTTTTGTTTCTGAAAATTCCAGAAAGGATATTTAAGAAAATACAATAAACTATTGGAAAGTACTCCCC TAACCTCTTTTCTGCATCATCTGTAGATACTAGCTATCTAGGTGGAGTTGAAAGAGTTAAGAATGTCGAT TAAAATCACTCTCAGTGCTTCTTACTATTAAGCAGTAAAAACTGTTCTCTATTAGACTTTAGAAATAAAT GTACCTGATGTACCTGATGCTATGGTCAGGTTATACTCCTCCTCCCCCAGCTATCTATATGGAATTGCTT ACCAAAGGATAGTGCGATGTTTCAGGAGGCTGGAGGAAGGGGGGTTGCAGTGGAGAGGGACAGCCCACTG AGAAGTCAAACATTTCAAAGTTTGGATTGTATCAAGTGGCATGTGCTGTGACCATTTATAATGTTAGTAG AAATTTTACAATAGGTGCTTATTCTCAAAGCAGGAATTGGTGGCAGATTTTACAAAAGATGTATCCTTCC AATTTGGAATCTTCTCTTTGACAATTCCTAGATAAAAAGATGGCCTTTGCTTATGAATATTTATAACAGC ATTCTTGTCACAATAAATGTATTCAAATACCAAAAAAAAAAAAAAAAA SEQ ID NO: 31 - Homo sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mRNA GGCCGCGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTGGCGGCGGCTCGGCCAGTA CTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGCAGGCACTGAAGGCGGCGGCGGGGCCAGA GGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGG AGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGAT CCAACAATAGAGGATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCG ACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCTTTCTTTG TGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAGAACAAATTAAAAGAGTT AAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTGCCTTCTAGAACAGTAGACA CAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCTTTTATTGAAACATC AGC AAAGACAAGACA GGG TGTTGAT GATGCCTTCTATACATTAGTTCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGAT GGTAAAAAGAAGAAAAAGAAGTCAAAGACAAAGTGTGTAATTATGTAAATACAATTTGTACTTTTTTCTT AAGGCATACTAGTACAAGTGGTAATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAGCATTACC TAATTTTTTTCCTGCTCCATGCAGACTGTTAGCTTTTACCTTAAATGCTTATTTTAAAATGACAGTGGAA GTTTTTTTTTCCTCTAAGTGCCAGTATTCCCAGAGTTTTGGTTTTTGAACTAGCAATGCCTGTGAAAAAG AAACTGAATACCTAAGATTTCTGTCTTGGGGTTTTTGGTGCATGCAGTTGATTACTTCTTATTTTTCTTA CCAATTGTGAATGTTGGTGTGAAACAAATTAATGAAGCTTTTGAATCATCCCTATTCTGTGTTTTATCTA GTCACATAAATGGATTAATTACTAATTTCAGTTGAGACCTTCTAATTGGTTTTTACTGAAACATTGAGGG AACACAAATTTATGGGCTTCCTGATGATGATTCTTCTAGGCATCATGTCCTATAGTTTGTCATCCCTGAT GAATGTAAAGTTACACTGTTCACAAAGGTTTTGTCTCCTTTCCACTGCTATTAGTCATGGTCACTCTCCC CAAAATATTATATTTTTTCTATAAAAAGAAAAAAATGGAAAAAAATTACAAGGCAATGGAAACTATTATA AGGCCATTTCCTTTTCACATTAGATAAATTACTATAAAGACTCCTAATAGCTTTTCCTGTTAAGGCAGAC CCAGTATGAAATGGGGATTATTATAGCAACCATTTTGGGGCTATATTTACATGCTACTAAATTTTTATAA TAATTGAAAAGATTTTAACAAGTATAAAAAATTCTCATAGGAATTAAATGTAGTCTCCCTGTGTCAGACT GCTCTTTCATAGTATAACTTTAAATCTTTTCTTCAACTTGAGTCTTTGAAGATAGTTTTAATTCTGCTTG TGACATTAAAAGATTATTTGGGCCAGTTATAGCTTATTAGGTGTTGAAGAGACCAAGGTTGCAAGGCCAG GCCCTGTGTGAACCTTTGAGCTTTCATAGAGAGTTTCACAGCATGGACTGTGTCCCCACGGTCATCCAGT GTTGTCATGCATTGGTTAGTCAAAATGGGGAGGGACTAGGGCAGTTTGGATAGCTCAACAAGATACAATC TCACTCTGTGGTGGTCCTGCTGACAAATCAAGAGCATTGCTTTTGTTTCTTAAGAAAACAAACTCTTTTT TAAAAATTACTTTTAAATATTAACTCAAAAGTTGAGATTTTGGGGTGGTGGTGTGCCAAGACATTAATTT TTTTTTTAAACAATGAAGTGAAAAAGTTTTACAATCTCTAGGTTTGGCTAGTTCTCTTAACACTGGTTAA ATTAACATTGCATAAACACTTTTCAAGTCTGATCCATATTTAATAATGCTTTAAAATAAAAATAAAAACA ATCCTTTTGATAAATTTAAAATGTTACTTATTTTAAAATAAATGAAGTGAGATGGCATGGTGAGGTGAAA GTATCACTGGACTAGGAAGAAGGTGACTTAGGTTCTAGATAGGTGTCTTTTAGGACTCTGATTTTGAGGA CATCACTTACTATCCATTTCTTCATGTTAAAAGAAGTCATCTCAAACTCTTAGTTTTTTTTTTTTACAAC TATGTAATTTATATTCCATTTACATAAGGATACACTTATTTGTCAAGCTCAGCACAATCTGTAAATTTTT AACCTATGTTACACCATCTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGAGGTGAAGTTTATATTTGAA TATCCATTCTCGTTTTAGGACTCTTCTTCCATATTAGTGTCATCTTGCCTCCCTACCTTCCACATGCCCC ATGACTTGATGCAGTTTTAATACTTGTAATTCCCCTAACCATAAGATTTACTGCTGCTGTGGATATCTCC ATGAAGTTTTCCCACTGAGTCACATCAGAAATGCCCTACATCTTATTTCCTCAGGGCTCAAGAGAATCTG ACAGATACCATAAAGGGATTTGACCTAATCACTAATTTTCAGGTGGTGGCTGATGCTTTGAACATCTCTT TGCTGCCCAATCCATTAGCGACAGTAGGATTTTTCAAACCTGGTATGAATAGACAGAACCCTATCCAGTG GAAGGAGAATTTAATAAAGATAGTGCTGAAAGAATTCCTTAGGTAATCTATAACTAGGACTACTCCTGGT AACAGTAATACATTCCATTGTTTTAGTAACCAGAAATCTTCATGCAATGAAAAATACTTTAATTCATGAA GCTTACTTTTTTTTTTTGGTGTCAGAGTCTCGCTCTTGTCACCCAGGCTGGAATGCAGTGGCGCCATCTC AGCTCACTGCAACCTCCATCTCCCAGGTTCAAGCGATTCTCGTGCCTCGGCCTCCTGAGTAGCTGGGATT ACAGGCGTGTGCCACTACACTCAACTAATTTTTGTATTTTTAGGAGAGACGGGGTTTCACCCTGTTGGCC AGGCTGGTCTCGAACTCCTGACCTCAAGTGATTCACCCACCTTGGCCTCATAAACCTGTTTTGCAGAACT CATTTATTCAGCAAATATTTATTGAGTGCCTACCAGATGCCAGTCACCGCACAAGGCACTGGGTATATGG TATCCCCAAACAAGAGACATAATCCCGGTCCTTAGGTAGTGCTAGTGTGGTCTGTAATATCTTACTAAGG CCTTTGGTATACGACCCAGAGATAACACGATGCGTATTTTAGTTTTGCAAAGAAGGGGTTTGGTCTCTGT GCCAGCTCTATAATTGTTTTGCTACGATTCCACTGAAACTCTTCGATCAAGCTACTTTATGTAAATCACT TCATTGTTTTAAAGGAATAAACTTGATTATATTGTTTTTTTATTTGGCATAACTGTGATTCTTTTAGGAC AATTACTGTACACATTAAGGTGTATGTCAGATATTCATATTGACCCAAATGTGTAATATTCCAGTTTTCT CTGCATAAGTAATTAAAATATACTTAAAAATTAATAGTTTTATCTGGGTACAAATAAACAGGTGCCTGAA CTAGTTCACAGACAAGGAAACTTCTATGTAAAAATCACTATGATTTCTGAATTGCTATGTGAAACTACAG ATCTTTGGAACACTGTTTAGGTAGGGTGTTAAGACTTACACAGTACCTCGTTTCTACACAGAGAAAGAAA TGGCCATACTTCAGGAACTGCAGTGCTTATGAGGGGATATTTAGGCCTCTTGAATTTTTGATGTAGATGG GCATTTTTTTAAGGTAGTGGTTAATTACCTTTATGTGAACTTTGAATGGTTTAACAAAAGATTTGTTTTT GTAGAGATTTTAAAGGGGGAGAATTCTAGAAATAAATGTTACCTAATTATTACAGCCTTAAAGACAAAAA TCCTTGTTGAAGTTTTTTTAAAAAAAGCTAAATTACATAGACTTAGGCATTAACATGTTTGTGGAAGAAT ATAGCAGACGTATATTGTATCATTTGAGTGAATGTTCCCAAGTAGGCATTCTAGGCTCTATTTAACTGAG TCACACTGCATAGGAATTTAGAACCTAACTTTTATAGGTTATCAAAACTGTTGTCACCATTGCACAATTT TGTCCTAATATATACATAGAAACTTTGTGGGGCATGTTAAGTTACAGTTTGCACAAGTTCATCTCATTTG TATTCCATTGATTTTTTTTTTCTTCTAAACATTTTTTCTTCAAACAGTATATAACTTTTTTTAGGGGATT TTTTTTTAGACAGCAAAAACTATCTGAAGATTTCCATTTGTCAAAAAGTAATGATTTCTTGATAATTGTG TAGTAATGTTTTTTAGAACCCAGCAGTTACCTTAAAGCTGAATTTATATTTAGTAACTTCTGTGTTAATA CTGGATAGCATGAATTCTGCATTGAGAAACTGAATAGCTGTCATAAAATGAAACTTTCTTTCTAAAGAAA GATACTCACATGAGTTCTTGAAGAATAGTCATAACTAGATTAAGATCTGTGTTTTAGTTTAATAGTTTGA AGTGCCTGTTTGGGATAATGATAGGTAATTTAGATGAATTTAGGGGAAAAAAAAGTTATCTGCAGATATG TTGAGGGCCCATCTCTCCCCCCACACCCCCACAGAGCTAACTGGGTTACAGTGTTTTATCCGAAAGTTTC CAATTCCACTGTCTTGTGTTTTCATGTTGAAAATACTTTTGCATTTTTCCTTTGAGTGCCAATTTCTTAC TAGTACTATTTCTTAATGTAACATGTTTACCTGGAATGTATTTTAACTATTTTTGTATAGTGTAAACTGA AACATGCACATTTTGTACATTGTGCTTTCTTTTGTGGGACATATGCAGTGTGATCCAGTTGTTTTCCATC ATTTGGTTGCGCTGACCTAGGAATGTTGGTCATATCAAACATTAAAAATGACCACTCTTTTAATTGAAAT TAACTTTTAAATGTTTATAGGAGTATGTGCTGTGAAGTGATCTAAAATTTGTAATATTTTTGTCATGAAC TGTACTACTCCTAATTATTGTAATGTAATAAAAATAGTTACAGTGACAAAAAAAAAAAAAAA SEQ ID NO: 32 - Homo sapiens leprecan-like 4 (LEPREL4), mRNA GCTTCCTGGGCTTCCCATCTCTGGCGGGAAGCGCTCCCCGACGCATTCTCTACCTAGGGGACACCCCCAA GGCAGGAGCCCGGGCCGACGGAGAGGACTTAACGACACTATCGGACCCTCTGGGAAAAGAGGGGAGACGT CGTGACCCAGGCCCCGCCCCACCTTGCCGCCTCGTGCCCGGCGCTAAGACCCAGCGGGCGCGCCGCCCGC CCGGGGCCCGGCCCTGTCCCCTTCCGTCCGCGGGGCAGCCAGCTCAGCTCCGGAGAGCCGGCGGCGCGGC GGGCATGGCTCGGGTGGCGTGGGGGCTGCTGTGGTTGCTGCTGGGCAGCGCCGGGGCGCAGTACGAGAAG TACAGCTTCCGGGGCTTCCCGCCCGAGGACCTGATGCCGCTGGCCGCGGCGTACGGGCACGCTCTGGAGC AGTACGAGGGAGAGAGCTGGCGCGAGAGCGCGCGCTACCTGGAGGCGGCGCTGCGGCTGCACCGGCTCCT GCGCGACAGCGAGGCCTTCTGCCACGCCAACTGCAGCGGCCCCGCGCCCGCGGCCAAGCCCGATCCCGAC GGCGGCCGCGCAGACGAGTGGGCCTGCGAGCTGCGGCTCTTCGGCCGCGTCCTGGAGCGAGCCGCCTGCC TGCGGCGCTGCAAGCGGACGCTGCCCGCCTTCCAGGTGCCCTACCCGCCGCGGCAGCTGCTGCGTGACTT CCAGAGCCGCCTGCCCTACCAGTACCTGCACTACGCGCTGTTCAAGGCTAACCGGCTGGAGAAGGCGGTG GCGGCGGCCTACACCTTCCTCCAGAGGAACCCGAAGCACGAGCTGACCGCCAAGTATCTCAACTACTATC AGGGGATGCTGGACGTCGCCGACGAGTCCCTCACGGACCTAGAGGCCCAGCCCTACGAGGCCGTGTTCCT CCGGGCTGTGAAGCTCTACAACAGCGGGGATTTCCGCAGCAGCACGGAGGACATGGAGCGGGCCTTGTCA GAGTACCTGGCAGTCTTTGCCCGGTGCCTGGCCGGCTGTGAAGGGGCCCATGAGCAGGTGGACTTCAAGG ACTTCTACCCGGCCATAGCAGATCTCTTTGCAGAGTCCCTGCAGTGCAAGGTGGACTGTGAGGCCAATTT GACCCCCAATGTGGGTGGCTACTTCGTGGACAAGTTCGTGGCCACCATGTACCACTACCTGCAGTTTGCC TACTATAAGTTGAATGATGTGCGCCAGGCTGCCCGCAGCGCCGCCAGCTACATGCTCTTCGACCCCAAGG ACAGCGTCATGCAGCAGAACCTGGTGTATTACCGGTTCCACCGGGCTCGCTGGGGCCTGGAAGAGGAGGA CTTCCAGCCCCGGGAGGAGGCCATGCTCTACCACAACCAGACCGCCGAGCTGCGGGAGCTGCTGGAGTTC ACCCACATGTACCTGCAGTCAGAT GAT GAGATGGAGCTGGAGGAGACAG AACCGCCCCTGGAGCCTGAGG ATGCCCTATCTGACGCCGAGTTTGAGGGGGAGGGTGACTACGAGGAGGGCATGTATGCTGACTGGTGGCA GGAGCCGGATGCCAAGGGTGACGAGGCCGAGGCTGAGCCAGAGCCTGAACTCGCATGAGAAGGGGACACC CCACACCGCTCAAGCTTGGGAAGCCTGGTGCCGATGGCCCCACCCTCACCAGCCTGGGCAGCAGCAAGAA CTATTTATTAAAAACTTAAGATGGGCCAGGTGCGGTGGCTCACACCTGTAATCCCAGCATTTTGGGAGGC CAAGGTGGGTGGATCACTTGAGGCCAGGAGTTCAAGACCAGCCTGGCCAACATGATGAGACCTCCGTCTC TACTAAAATACATAAATTAGCCGGGTGTGGTGGCAGGCGCCTGAAATCCCAGCTACTCAAGAGGCTGAGG CAGGAGAATCGCTTGAACCTGGGAGGCAAAGGTTGCAGTGAACTGAGATTGCGCCACCGCACTCCAGCCT GGGCGACAGAGCGAGACTCCATCTTTAAAAAAAAACAAGACGGGCCGGCACGGTGGCTCACGCCTGTAAT CCCAGCACTGAGAGGCCGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCC ATCTCTACTAAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAATCGTAGCTGAGGCAGGA GAATCGCCTGAACCCAGGAGGCGGAGCTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGCG ACAGAGTGAGACTCCATCTCAAAAAAAAAAAAAAAAACTTAAGATGGACACAGCTGACTGGACCCCCATC CTGCCTCACCCATGGGTGCTGCACCCCAGACCCATCCTGCCACTTCTATGTCTCTGGACCACAGGATGGT GGTGGCATTGCAGGTTGGCAAGTGGGCTGATGGGGTCCGCCCTCCTCACTGCTGAGCTCCTCACCTGGAC AGTCTCCTGGACAAGGAGTTTCCAGCTGCTGGCTGGAGTCTCAGGCCAAATTGCAGAGGGTCCTCCAGGG TCCTGAAGAGCACTGGACTAAGAGTCTAGTGGTTCCAGGGCCCTGACCAGTAGGTGCTCAATAAATGTTT GTTGTTGAATGAAAAAAAAAAAAAAAAAA SEQ ID NO: 33 - Homo sapiens lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 2, mRNA GGAGGTGAGCAGGAAGGAGACGGCCGCCCAGCAGCCCGTGGGCAGGCGCGGCGGAGCGAGCGGGGCCGGC GGCGGGCGCCGAGGGACGCCGAGGCCTCGGGCGGGGGCTGGCCCGGGGTTCCAGGTCTCCAGTGGGGGCT GCAGACTAAGCAAAATGAGGCGGTTCCTGAGGCCAGGGCATGACCCTGTGCGGGAGAGGCTCAAGCGGGA CCTGTTCCAGTTTAACAAGACGGTGGAGCATGGCTTCCCGCACCAGCCCAGCGCCCTCGGCTACAGCCCG TCCCTGCGCATCCTGGCCATCGGCACCCGTTCTGGAGCCATCAAGCTCTACGGAGCCCCAGGCGTGGAGT TCATGGGGCTGCACCAGGAGAACAACGCTGTGACGCAGATCCACCTCCTGCCCGGCCAGTGCCAGCTGGT CACCCTGCTGGATGACAACAGCCTGCACCTTTGGAGCCTGAAGGTCAAGGGCGGGGCATCGGAGCTGCAG GAGGATGAGAGCTTCACACTGCGTGGACCCCCAGGGGCTGCCCCCAGTGCCACACAGATCACCGTGGTCC TGCCACATTCCTCCTGCGAGCTGCTCTACCTGGGCACCGAGAGTGGCAACGTGTTTGTGGTGCAGCTGCC AGCTTTTCGTGCGCTGGAGGACCGGACCATCAGCTCGGACGCGGTGC TGC AGCGGTTGCCAGAGGAGGCC CG CCACCGGCGTGTGTTCGAGATGGTGGAGGCACTGCAGGAGCACCCTCGAGACCCCAACCAGATCCTGA TCGGCTACAGCCGAGGCCTCGTTGTCATCTGGGACCTACAGGGCAGCCGCGTGCTCTACCACTTCCTCAG CAGCCAGCAACTGGAGAACATCTGGTGGCAGCGGGACGGCCGCCTGCTCGTCAGCTGTCACTCTGACGGC AGCTACTGCCAGTGGCCCGTGTCCAGCGAAGCCCAGCAACCAGAGCCCCTCCGCAGCCTCGTGCCTTACG GTCCCTTTCCTTGCAAAGCGATTACCAGAATCCTCTGGCTGACCACTAGGCAGGGGTTGCCCTTCACCAT CTTCCAGGGTGGCATGCCACGGGCCAGCTACGGGGACCGCCACTGCATCTCAGTGATCCACGATGGCCAG CAGACGGCCTTCGACTTCACCTCCCGTGTCATCGGCTTCACTGTCCTCACAGAGGCAGACCCTGCAGCCA GTAGGAGAGCTTCGGGAGTGGGTGCCCAGGGTTAGGTGTGGGAGGCATGGGGCAGGACCATCAGTAAAGA CAGGGCCAGGTGCAGTGGCTCCTGCCTGTAACCCCAGTGCTGTGGGAGGCCAAGGTGGTAGGATCGCTTG AACCCAGGAGTTCAAGTCCAGCCTGGACAACGTAGGGAGACCCTTGTCTCTACAAAAAATAAAAAAATTA GCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 34 - Homo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), mRNA GAAACGTCCCGTGTGGGAGGGGCGGGTCTGGGTGCGGCCTGCCGCATGACTCGTGGTTCGGAGGCCCACG TGGCCGGGGCGGGGACTCAGGCGCCTGGGGCGCCGACTGATTACGTAGCGGGCGGGGCCGGAAGTGCCGC TCCTTGGTGGGGGCTGTTCATGGCGGTTCCGGGGTCTCCAACATTTTTCCCGGCTGTGGTCCTAAATCTG TCCAAAGCAGAGGCAGTGGAGCTTGAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTGGT TGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACAATCCAGCTAATCCAGAACCACTTTGTAGATGAATAT GATCCCACCATAGAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACCTGTTTGTTGGACATAC TGGATACAGCTGGACAAGAAGAGTACAGTGCCATGAGAGACCAATACATGAGGACAGGCGAAGGCTTCCT CTGTGTATTTGCCATCAATAATAGCAAGTCATTTGCGGATATTAACCTCTACAGGGAGCAGATTAAGCGA GTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGATTTGCCAACAAGGACAGTTG ATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATTCATTGAAACCT CAG CCAAGACCAG ACA GGGTGTTGA AGATGCTTTTTACACACTGGTAAGAGAAATACGCCAGTACCGAATGAAAAAACTCAAC AGCAGTGATGATGGGACTCAGGGTTGTATGGGATTGCCATGTGTGGTGATGTAACAAGATACTTTTAAAG TTTTGTCAGAAAAGAGCCACTTTCAAGCTGCACTGACACCCTGGTCCTGACTTCCCTGGAGGAGAAGTAT TCCTGTTGCTGTCTTCAGTCTCACAGAGAAGCTCCTGCTACTTCCCCAGCTCTCAGTAGTTTAGTACAAT AATCTCTATTTGAGAAGTTCTCAGAATAACTACCTCCTCACTTGGCTGTCTGACCAGAGAATGCACCTCT TGTTACTCCCTGTTATTTTTCTGCCCTGGGTTCTTCCACAGCACAAACACACCTCTGCCACCCCAGGTTT TTCATCTGAAAAGCAGTTCATGTCTGAAACAGAGAACCAAACCGCAAACGTGAAATTCTATTGAAAACAG TGTCTTGAGCTCTAAAGTAGCAACTGCTGGTGATTTTTTTTTTCTTTTTACTGTTGAACTTAGAACTATG CTAATTTTTGGAGAAATGTCATAAATTACTGTTTTGCCAAGAATATAGTTATTATTGCTGTTTGGTTTGT TTATAATGTTATCGGCTCTATTCTCTAAACTGGCATCTGCTCTAGATTCATAAATACAAAAATGAATACT GAATTTTGAGTCTATCCTAGTCTTCACAACTTTGACGTAATTAAATCCAACTTTCACAGTGAAGTGCCTT TTTCCTAGAAGTGGTTTGTAGACTTCCTTTATAATATTTCAGTGGAATAGATGTCTCAAAAATCCTTATG CATGAAATGAATGTCTGAGATACGTCTGTGACTTATCTACCATTGAAGGAAAGCTATATCTATTTGAGAG CAGATGCCATTTTGTACATGTATGAAATTGGTTTTCCAGAGGCCTGTTTTGGGGCTTTCCCAGGAGAAAG ATGAAACTGAAAGCACATGAATAATTTCACTTAATAATTTTTACCTAATCTCCACTTTTTTCATAGGTTA CTACCTATACAATGTATGTAATTTGTTTCCCCTAGCTTACTGATAAACCTAATATTCAATGAACTTCCAT TTGTATTCAAATTTGTGTCATACCAGAAAGCTCTACATTTGCAGATGTTCAAATATTGTAAAACTTTGGT GCATTGTTATTTAATAGCTGTGATCAGTGATTTTCAAACCTCAAATATAGTATATTAACAAATTACATTT TCACTGTATATCATGGTATCTTAATGATGTATATAATTGCCTTCAATCCCCTTCTCACCCCACCCTCTAC AGCTTCCCCCACAGCAATAGGGGCTTGATTATTTCAGTTGAGTAAAGCATGGTGCTAATGGACCAGGGTC ACAGTTTCAAAACTTGAACAATCCAGTTAGCATCACAGAGAAAGAAATTCTTCTGCATTTGCTCATTGCA CCAGTAACTCCAGCTAGTAATTTTGCTAGGTAGCTGCAGTTAGCCCTGCAAGGAAAGAAGAGGTCAGTTA GCACAAACCCTTTACCATGACTGGAAAACTCAGTATCACGTATTTAAACATTTTTTTTTCTTTTAGCCAT GTAGAAACTCTAAATTAAGCCAATATTCTCATTTGAGAATGAGGATGTCTCAGCTGAGAAACGTTTTAAA TTCTCTTTATTCATAATGTTCTTTGAAGGGTTTAAAACAAGATGTTGATAAATCTAAGCTGATGAGTTTG CTCAAAACAGGAAGTTGAAATTGTTGAGACAGGAATGGAAAATATAATTAATTGATACCTATGAGGATTT GGAGGCTTGGCATTTTAATTTGCAGATAATACCCTGGTAATTCTCATGAAAAATAGACTTGGATAACTTT TGATAAAAGACTAATTCCAAAATGGCCACTTTGTTCCTGTCTTTAATATCTAAATACTTACTGAGGTCCT CCATCTTCTATATTATGAATTTTCATTTATTAAGCAAATGTCATATTACCTTGAAATTCAGAAGAGAAGA AACATATACTGTGTCCAGAGTATAATGAACCTGCAGAGTTGTGCTTCTTACTGCTAATTCTGGGAGCTTT CACAGTACTGTCATCATTTGTAAATGGAAATTCTGCTTTTCTGTTTCTGCTCCTTCTGGAGCAGTGCTAC TCTGTAATTTTCCTGAGGCTTATCACCTCAGTCATTTCTTTTTTAAATGTCTGTGACTGGCAGTGATTCT TTTTCTTAAAAATCTATTAAATTTGATGTCAAATTAGGGAGAAAGATAGTTACTCATCTTGGGCTCTTGT GCCAATAGCCCTTGTATGTATGTACTTAGAGTTTTCCAAGTATGTTCTAAGCACAGAAGTTTCTAAATGG GGCCAAAATTCAGACTTGAGTATGTTCTTTGAATACCTTAAGAAGTTACAATTAGCCGGGCATGGTGGCC CGTGCCTGTAGTCCCAGCTACTTGAGAGGCTGAGGCAGGAGAATCACTTCAACCCAGGAGGTGGAGGTTA CAGTGAGCAGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAAGAGAGACTTGTCTCCAAAAAAAAAGT TACACCTAGGTGTGAATTTTGGCACAAAGGAGTGACAAACTTATAGTTAAAAGCTGAATAACTTCAGTGT GGTATAAAACGTGGTTTTTAGGCTATGTTTGTGATTGCTGAAAAGAATTCTAGTTTACCTCAAAATCCTT CTCTTTCCCCAAATTAAGTGCCTGGCCAGCTGTCATAAATTACATATTCCTTTTGGTTTTTTTAAAGGTT ACATGTTCAAGAGTGAAAATAAGATGTTCTGTCTGAAGGCTACCATGCCGGATCTGTAAATGAACCTGTT AAATGCTGTATTTGCTCCAACGGCTTACTATAGAATGTTACTTAATACAATATCATACTTATTACAATTT TTACTATAGGAGTGTAATAGGTAAAATTAATCTCTATTTTAGTGGGCCCATGTTTAGTCTTTCACCATCC TTTAAACTGCTGTGAATTTTTTTGTCATGACTTGAAAGCAAGGATAGAGAAACACTTTAGAGATATGTGG GGTTTTTTTACCATTCCAGAGCTTGTGAGCATAATCATATTTGCTTTATATTTATAGTCATGAACTCCTA AGTTGGCAGCTACAACCAAGAACCAAAAAATGGTGCGTTCTGCTTCTTGTAATTCATCTCTGCTAATAAA TTATAAGAAGCAAGGAAAATTAGGGAAAATATTTTATTTGGATGGTTTCTATAAACAAGGGACTATAATT CTTGTACATTATTTTTCATCTTTGCTGTTTCTTTGAGCAGTCTAATGTGCCACACAATTATCTAAGGTAT TTGTTTTCTATAAGAATTGTTTTAAAAGTATTCTTGTTACCAGAGTAGTTGTATTATATTTCAAAACGTA AGATGATTTTTAAAAGCCTGAGTACTGACCTAAGATGGAATTGTATGAACTCTGCTCTGGAGGGAGGGGA GGATGTCCGTGGAAGTTGTAAGACTTTTATTTTTTTGTGCCATCAAATATAGGTAAAAATAATTGTGCAA TTCTGCTGTTTAAACAGGAACTATTGGCCTCCTTGGCCCTAAATGGAAGGGCCGATATTTTAAGTTGATT ATTTTATTGTAAATTAATCCAACCTAGTTCTTTTTAATTTGGTTGAATGTTTTTTCTTGTTAAATGATGT TTAAAAAATAAAAACTGGAAGTTCTTGGCTTAGTCATAATTCTT SEQ ID NO: 35 - Homo sapiens 2′-5′-oligoadenylate synthetase 1, 40/46 kDa (OAS1), transcript variant 3, mRNA TCCCTTCTGAGGAAACGAAACCAACAGCAGTCCAAGCTCAGTCAGCAGAAGAGATAAAAGCAAACAGGTC TGGGAGGCAGTTCTGTTGCCACTCTCTCTCCTGTCAATGATGGATCTCAGAAATACCCCAGCCAAATCTC TGGACAAGTTCATTGAAGACTATCTCTTGCCAGACACGTGTTTCCGCATGCAAATCAACCATGCCATTGA CATCATCTGTGGGTTCCTGAAGGAAAGGTGCTTCCGAGGTAGCTCCTACCCTGTGTGTGTGTCCAAGGTG GTAAAGGGTGGCTCCTCAGGCAAGGGCACCACCCTCAGAGGCCGATCTGACGCTGACCTGGTTGTCTTCC TCAGTCCTCTCACCACTTTTCAGGATCAGTTAAATCGCCGGGGAGAGTTCATCCAGGAAATTAGGAGACA GCTGGAAGCCTGTCAAAGAGAGAGAGCATTTTCCGTGAAGTTTGAGGTCCAGGCTCCACGCTGGGGCAAC CCCCGTGCGCTCAGCTTCGTACTGAGTTCGCTCCAGCTCGGGGAGGGGGTGGAGTTCGATGTGCTGCCTG CCTTT GAT GCCCTGGGTCAGTTGACTGGCG GCTATAAACCTAACCCCCAAATCTATGTCAAGCTCATCGA GGAGTGCACCGACCTGCAGAAAGAGGGCGAGTTCTCCACCTGCTTCACAGAACTACAGAGAGACTTCCTG AAGCAGCGCCCCACCAAGCTCAAGAGCCTCATCCGCCTAGTCAAGCACTGGTACCAAAATTGTAAGAAGA AGCTTGGGAAGCTGCCACCTCAGTATGCCCTGGAGCTCCTGACGGTCTATGCTTGGGAGCGAGGGAGCAT GAAAACACATTTCAACACAGCCCAGGGATTTCGGACGGTCTTGGAATTAGTCATAAACTACCAGCAACTC TGCATCTACTGGACAAAGTATTATGACTTTAAAAACCCCATTATTGAAAAGTACCTGAGAAGGCAGCTCA CGAAACCCAGGCCTGTGATCCTGGACCCGGCGGACCCTACAGGAAACTTGGGTGGTGGAGACCCAAAGGG TTGGAGGCAGCTGGCACAAGAGGCTGAGGCCTGGCTGAATTACCCATGCTTTAAGAATTGGGATGGGTCC CCAGTGAGCTCCTGGATTCTGCTGACCCAGCACACTCCAGGCAGCATCCACCCCACAGGCAGAAGAGGAC TGGACCTGCACCATCCTCTGAATGCCAGTGCATCTTGGGGGAAAGGGCTCCAGTGTTATCTGGACCAGTT CCTTCATTTTCAGGTGGGACTCTTGATCCAGAGAGGACAAAGCTCCTCAGTGAGCTGGTGTATAATCCAG GACAGAACCCAGGTCTCCTGACTCCTGGCCTTCTATGCCCTCTATCCTATCATAGATAACATTCTCCACA GCCTCACTTCATTCCACCTATTCTCTGAAAATATTCCCTGAGAGAGAACAGAGAGATTTAGATAAGAGAA TGAAATTCCAGCCTTGACTTTCTTCTGTGCACCTGATGGGAGGGTAATGTCTAATGTATTATCAATAACA ATAAAAATAAAGCAAATACCATTTAAAAAAAAAAA SEQ ID NO: 36 - Homo sapiens origin recognition complex, subunit 1 (ORC1), transcript variant 3, mRNA ACGGTCTGGGGGCGGGGCCACGCCGATTGGCGCGAAGTTTTCTTTTCTCCTTCCACCTTCTTTTCATTTC TAGTGAGACACACGCTTTGGTCCTGGCTTTCGGCCCGTAGTTGTAGAAGGAGCCCTGCTGGTGCAGGTTA GAGGTGCCGCATCCCCCGGAGCTCTCGAAGTGGAGGCGGTAGGAAACGGAGGGCTTGCGGCTAGCCGGAG GAAGC TTT GGAGCCGGAAGCCATGGCACAC TACCCCACAAGGCTGAAGACCAGAAAAACTTATTCATGGG TTGGCAGGCCCTTGTTGGATCGAAAACTGCACTACCAAACCTATAGAGAAATGTGTGTGAAAACAGAAGG TTGTTCCACCGAGATTCACATCCAGATTGGACAGTTTGTGTTGATTGAAGGGGATGATGATGAAAACCCG TATGTTGCTAAATTGCTTGAGTTGTTCGAAGATGACTCTGATCCTCCTCCTAAGAAACGTGCTCGAGTAC AGTGGTTTGTCCGATTCTGTGAAGTCCCTGCCTGTAAACGGCATTTGTTGGGCCGGAAGCCTGGTGCACA GGAAATATTCTGGTATGATTACCCGGCCTGTGACAGCAACATTAATGCGGAGACCATCATTGGCCTTGTT CGGGTGATACCTTTAGCCCCAAAGGATGTGGTACCGACGAATCTGAAAAATGAGAAGACACTCTTTGTGA AACTATCCTGGAATGAGAAGAAATTCAGGCCACTTTCCTCAGAACTATTTGCGGAGTTGAATAAACCACA AGAGAGTGCAGCCAAGTGCCAGAAACCCGTGAGAGCCAAGAGTAAGAGTGCAGAGAGCCCTTCTTGGACC CCAGCAGAACATGTGGCCAAAAGGATTGAATCAAGGCACTCCCCCTCCAAATCTCGCCAAACTCCTACCC ATCCTCTTACCCCAAGAGCCAGAAAGAGGCTGGAGCTTGGCAACTTAGGTAACCCTCAGATGTCCCAGCA GACTTCATGTGCCTCCTTGGATTCTCCAGGAAGAATAAAACGGAAAGTGGCCTTCTCGGAGATCACCTCA CCTTCTAAGAGATCTCAGCCTGATAAACTTCAAACCTTGTCTCCAGCTCTGAAAGCCCCAGAGAAAACCA GAGAGACTGGACTCTCTTATACTGAGGATGACAAGAAGGCTTCACCTGAACATCGCATAATCCTGAGAAC CCGAATTGCAGCTTCGAAAACCATAGACATTAGAGAGGAGAGAACACTTACCCCTATCAGTGGGGGACAG AGATCTTCAGTGGTGCCATCCGTGATTCTGAAACCAGAAAACATCAAAAAGAGGGATGCAAAAGAAGCAA AAGCCCAGAATGAAGCGACCTCTACTCCCCATCGTATCCGCAGAAAGAGTTCTGTCTTGACTATGAATCG GATTAGGCAGCAGCTTCGGTTTCTAGGTAATAGTAAAAGTGACCAAGAAGAGAAAGAGATTCTGCCAGCA GCAGAGATTTCAGACTCTAGCAGTGACGAAGAAGAGGCTTCCACACCGCCCCTTCCAAGGAGAGCACCCA GAACTGTGTCCAGGAACCTGCGATCTTCCTTGAAGTCATCCTTACATACCCTCACGAAGCTCAAGCCTAG AACGCCACGTTGTGCCGCTCCTCAGATCCGTAGTCGAAGCCTGGCTGCCCAGGAGCCAGCCAGTGTGCTG GAGGAAGCCCGACTGAGGCTGCATGTTTCTGCTGTACCTGAGTCTCTTCCCTGTCGGGAACAGGAATTCC AAGACATCTACAATTTTGTGGAAAGCAAACTCCTTGACCATACCGGAGGGTGCATGTACATCTCCGGTGT CCCTGGGACAGGGAAGACTGCCACTGTTCATGAAGTGATACGCTGCCTGCAGCAGGCAGCCCAAGCCAAT GATGTTCCTCCCTTTCAATACATTGAGGTCAATGGCATGAAGCTGACGGAGCCCCACCAAGTCTATGTGC AAATCTTGCAGAAGCTAACAGGCCAAAAAGCAACAGCCAACCATGCGGCAGAACTGCTGGCAAAGCAATT CTGCACCCGAGGGTCACCTCAGGAAACCACCGTCCTGCTTGTGGATGAGCTCGACCTTCTGTGGACTCAC AAACAAGACATAATGTACAATCTCTTTGACTGGCCCACTCATAAGGAGGCCCGGCTTGTGGTCCTGGCAA TTGCCAACACAATGGACCTGCCAGAGCGAATCATGATGAACCGGGTGTCCAGCCGACTGGGTCTTACCAG GATGTGCTTCCAGCCCTATACATATAGCCAGCTGCAGCAGATCCTAAGGTCCCGGCTCAAGCATCTAAAG GCCTTTGAAGATGATGCCATCCAGCTGGTAGCCAGGAAGGTAGCAGCACTGTCTGGAGATGCACGACGGT GCCTGGACATCTGCAGGCGTGCCACAGAGATCTGTGAGTTCTCCCAGCAGAAGCCTGACTCCCCTGGCCT GGTCACCATAGCCCACTCAATGGAAGCTGTGGATGAGATGTTTTCATCATCATACATCACGGCCATCAAA AATTCCTCTGTTCTGGAACAGAGCTTCCTGAGAGCCATCCTCGCAGAGTTCCGTCGATCAGGACTGGAGG AAGCCACGTTTCAACAGATATATAGTCAACATGTGGCACTGTGCAGAATGGAGGGACTGCCGTACCCCAC CATGTCAGAGACCATGGCCGTGTGTTCTCACCTGGGCTCCTGTCGCCTCCTGCTTGTGGAGCCCAGCAGG AACGATCTGCTCCTTCGGGTGCGGCTCAACGTCAGCCAGGATGATGTGCTGTATGCGCTGAAAGACGAGT AAAGGGGCTTCACAAGTTAAAAGACTGGGGTCTTGCTGGGTTTTGTTTTTTGAGACAGGGTCTTGCTCTG TCGCCCAGGCTGGAGTGCAGTGGCACGATCATGGCTCACTGCAGCCTTGACTTCTCAGGCTTAGGTGACC CCCCAACCTCATCCTCCCAGGTGGCTGAAACTACAGGCACATGCCACCATGCCCAGCTGATTTTTTGTAG AGACAGGGCTTCACCATGTTGCCAAGCTAGTCTACAAAGCATCTGATTTTGGAAGTACATGGAATTGTTG TAACAAAGTATATTGAATGGAAATGGCTCTCATGTATTTTGGAATTTTCCATTAAATAATTTGCTTTTTC CTGAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 37 - Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA GAGAGCAGCGGCCGGGAAGGGGCGGTGCGGGAGGCGGGGTGTGGGGCGGTAGTGTGGGCCCTGTTCCTGC CCGCGCGGTGTTCCGCATTCTGCAAGCCTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAAT CACCGACCTCTCTCCCCAGCTGTATTTCCAAAATGTCGCTTTCTAACAAGCTGACGCTGGACAAGCTGGA CGTTAAAGGGAAGCGGGTCGTTATGAGAGTCGACTTCAATGTTCCTATGAAGAACAACCAGATAACAAAC AACCAGAGGATTAAGGCTGCTGTCCCAAGCATCAAATTCTGCTTGGACAATGGAGCCAAGTCGGTAGTCC TTATGAGCCACCTAGGCCGGCCTGATGGTGTGCCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGT AGAACTCAAATCTCTGCTGGGCAAGGATGTTCTGTTCTTGAAGGACTGTGTAGGCCCAGAAGTGGAGAAA GCCTGTGCCAACCCAGCTGCTGGGTCTGTCATCCTGCTGGAGAACCTCCGCTTTCATGTGGAGGAAGAAG GGAAGGGAAAAGATGCTTCTGGGAA CAA GGTTAAAGCCGAGCCAGCCAAA ATAGAAGCTTTCCGAGCTTC ACTTTCCAAGCTAGGGGATGTCTATGTCAATGATGCTTTTGGCACTGCTCACAGAGCCCACAGCTCCATG GTAGGAGTCAATCTGCCACAGAAGGCTGGTGGGTTTTTGATGAAGAAGGAGCTGAACTACTTTGCAAAGG CCTTGGAGAGCCCAGAGCGACCCTTCCTGGCCATCCTGGGCGGAGCTAAAGTTGCAGACAAGATCCAGCT CATCAATAATATGCTGGACAAAGTCAATGAGATGATTATTGGTGGTGGAATGGCTTTTACCTTCCTTAAG GTGCTCAACAACATGGAGATTGGCACTTCTCTGTTTGATGAAGAGGGAGCCAAGATTGTCAAAGACCTAA TGTCCAAAGCTGAGAAGAATGGTGTGAAGATTACCTTGCCTGTTGACTTTGTCACTGCTGACAAGTTTGA TGAGAATGCCAAGACTGGCCAAGCCACTGTGGCTTCTGGCATACCTGCTGGCTGGATGGGCTTGGACTGT GGTCCTGAAAGCAGCAAGAAGTATGCTGAGGCTGTCACTCGGGCTAAGCAGATTGTGTGGAATGGTCCTG TGGGGGTATTTGAATGGGAAGCTTTTGCCCGGGGAACCAAAGCTCTCATGGATGAGGTGGTGAAAGCCAC TTCTAGGGGCTGCATCACCATCATAGGTGGTGGAGACACTGCCACTTGCTGTGCCAAATGGAACACGGAG GATAAAGTCAGCCATGTGAGCACTGGGGGTGGTGCCAGTTTGGAGCTCCTGGAAGGTAAAGTCCTTCCTG GGGTGGATGCTCTCAGCAATATTTAGTACTTTCCTGCCTTTTAGTTCCTGTGCACAGCCCCTAAGTCAAC TTAGCATTTTCTGCATCTCCACTTGGCATTAGCTAAAACCTTCCATGTCAAGATTCAGCTAGTGGCCAAG AGATGCAGTGCCAGGAACCCTTAAACAGTTGCACAGCATCTCAGCTCATCTTCACTGCACCCTGGATTTG CATACATTCTTCAAGATCCCATTTGAATTTTTTAGTGACTAAACCATTGTGCATTCTAGAGTGCATATAT TTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTTAGCTTAGTTCTCTTTTGATGTAGGTTATTATGA TTAGCTTTGTCACTGTTTCACTACTCAGCATGGAAACAAGATGAAATTCCATTTGTAGGTAGTGAGACAA AATTGATGATCCATTAAGTAAACAATAAAAGTGTCCATTGAAACCGTGATTTTTTTTTTTTTCCTGTCAT ACTTTGTTAGGAAGGGTGAGAATAGAATCTTGAGGAACGGATCAGATGTCTATATTGCTGAATGCAAGAA GTGGGGCAGCAGCAGTGGAGAGATGGGACAATTAGATAAATGTCCATTCTTTATCAAGGGCCTACTTTAT GGCAGACATTGTGCTAGTGCTTTTATTCTAACTTTTATTTTTATCAGTTACACATGATCATAATTTAAAA AGTCAAGGCTTATAACAAAAAAGCCCCAGCCCATTCCTCCCATTCAAGATTCCCACTCCCCAGAGGTGAC CACTTTCAACTCTTGAGTTTTTCAGGTATATACCTCCATGTTTCTAAGTAATATGCTTATATTGTTCACT TCTTTTTTTTTTATTTTTTAAAGAAATCTATTTCATACCATGGAGGAAGGCTCTGTTCCACATATATTTC CACTTCTTCATTCTCTCGGTATAGTTTTGTCACAATTATAGATTAGATCAAAAGTCTACATAACTAATAC AGCTGAGCTATGTAGTATGCTATGATTAAATTTACTTATGTAAAAAAAAAAAAAAAAAA SEQ ID NO: 38 - Homo sapiens phorbol-12-myristate-13-acetate- induced protein 1 (PMAIP1), mRNA ACTGGACAAAAGCGTGGTCTCTGGCGCGGGGATCTCAGAGTTTCCCGGGCACTCACCGTGTGTAGTTGGC ATCTCCGCGCGTCCGGACACCCGATCCCAGCATCCCTGCCTGCAGGACTGTTCGTGTTCAGCTCGCGTCC TGCAGCTGTCCGAGGTGCTCCAGTTGGAGGCTGAGGTTCCCGGGCTCTGTAGCTGAGTGGGCGGCGGCAC CGGCGGAGATGCCTGGGAAGAAGGCGCGCAAGAACGCTCAACCGAGCCCCGCGC GGG CTCCAGCAGAGCT GGA AGTCGA GTGTGCTACTCAACTCAGGAGATTTGGAGACAAACTGAACTTCCGGCAGAAACTTCTGAAT CTGATATCCAAACTCTTCTGCTCAGGAACCTGACTGCATCAAAAACTTGCATGAGGGGACTCCTTCAAAA GAGTTTTCTCAGGAGGTGCACGTTTCATCAATTTGAAGAAAGACTGCATTGTAATTGAGAGGAATGTGAA GGTGCATTCATGGGTGCCCTTGGAAACGGAAGATGGAATACATCAAAGTGAATTTCTGTTCAAGTTTTCC CAGATTATCATTCTTTGGGATGAGAGAACATTATAAAACCACTTTGTTTATTTTAAAGCAAGAATGGAAG ACCCTTGAAAATAAAGAAGTAATTATTGACACATTTCTTTTTTACTTAGAGAATCGTTCTAGTGTTTTTG CCGAAGATTACCGCTGGCCTACTGTGAAGGGAGATGACCTGTGATTAGACTGGGCGGCTGGGGAGAAACA GTTCAGTGCATTGTTGTTGTTGCTGTTTTTGGTGTTTTGCTTTTCAGTGCCAACTCAGCACATTGTATAT GATTCGGTTTATACATATTACCTTGTTATAATGAAAAAACTCATTCTGAGAACACTGAAATGTTATACTC AGTGTTGATTTCTTCGGTCACTACACAACGTAAAATCATTTGTTTCTTTTGACTCAAATTGTATTGCTTC TGTTCAGATGATCTTTCATTCAATGTGTTCCTGTTGGGCGTTACTAGAAACTATGGAAAACTGGAAAATA ACTTTGAAAAAATTGGATAAAGTATAGGAGGGTTACTTGGGGCCAGTAAATCAGTAGACTGAACATTCAA TATAATAAAAGAACATGGGGATTTTGTATAACCAGGGATAATAAAAAGAAAAAAGAAGTTAATTTTTAAT TGATGTTTTTGAAACTTAGTAGAACAAATATTCAGAAGTAACTTGATAAGATATGAATGTTTCTAAAGAA GTTTCTAAAGGTTCGGAAAATGCTCCTTGTCACATTAGTGTGCATCCTACAAAAAGTGATCTCTTAATGT AAATTAAGAATATTTTCATAATTGGAATATACTTTTCTTAAAAAAAAGGAACAGTTAGTTCTCATCTAGA ATGAAAGTTCCATATATGCATTGGTGAATATATATGTATACACATACTTACATACTTATATGGGTATCTG TATAGATAATTTGTATTAGAGTATTATATAGCTTCTTAGTAGGGTCTCAAGTAAGTTTCATTTTTTTTAT CTGGGCTATATACAGTCCTCAAATAAATAATGTCTTGATTTTATTTCAGCAGGAATAATTTTATTTATTT TGCCTATTTATAATTAAAGTATTTTTCTTTAGTTTGAAAATGTGTATTAAAGTTACATTTTTGAGTTACA AGAGTCTTATAACTACTTGAATTTTTAGTTAAAATGTCTTAATGTAGGTTGTAGTCACTTTAGATGGAAA ATTACCTCACATCTGTTTTCTTCAGTATTACTTAAGATTGTTTATTTAGTGGTAGAGAGTTTTTTTTTTC AGCCTAGAGGCAGCTATTTTACCATCTGGTATTTATGGTCTAATTTGTATTTAAACATATGCACACATAT AAAAGTTGATACTGTGGCAGTAAACTATTAAAAGTTTTCACTGTTCAAAAAAAAAAAAAAAAAA SEQ ID NO: 39 - Homo sapiens POU class 6 homeobox 1 (POU6F1), transcript variant 2, non-coding RNA AATCGGTGGCCGCCAGACACCCGCGGCGAAGGCGGCTCGGGCTCGGGCTCCGGATGTGCTAGGTGTGGGC CGGCCCCCACCCGACCCTGACAAGTGACCATGGATCCTGGAGCCGGGTCAGAGACATCTCTGACTGTCAA TGAGCAGGTCATCGTGATGTCAGGTCATGAGACCATCCGAGTGCTGGAAGTCGGAGTGGATGCCCAACTC CCTGCTGAGGAAGAGAGCAAAGGACTGGAGGGTGTGGCCGCCGAGGGCTCCCAGAGCGGAGACCCTGCTG AAGCCAGTCAAGCTGCTGGTGAAGCTGGGCCAGACAACCTGGGCTCCTCTGCAGAGGCAACTGTGAAGTC ACCCCCGGGGATCCCTCCGAGCCCTGCCCCTGCCATTGCCACCTTCAGCCAAGCCCCAAGCCAGCCTCAG GCATCGCAGACCCTGACGCCACTGGCTGTACAAGCTGCCCCCCAGTATTGCAGGTCAAGTGGCTGGTCAG CAGGGGCTGGCCGTGTGGACAATTCCTACAGCAACTGTGGCTGCCCTCCCAGGACTGACCGCTGCTTCTC CTACGGGGGGAGTGTTCAAGCCACCTTTAGCCGGTCTCCAAGCAGCTGCTGTGCTGAACACCGCTCTTCC GGCACCGGTACAAGCTGCCGCACCAGTACAGGCCTCCTCGACGGCCCAACCCCGGCCACCAGCCCAGCCC CAGACGCTGTTCCAGACCCAGCCGCTGCTGCAGACCACACCTGCCATCCTCCCGCAGCCCACTGCTGCCA CCGCTGCTGCCCCTACCCCCAAGCCAGTGGACACCCCCCCACAGATCACCGTCCAGCCTGCAGGCTTCGC ATTTAGCCCAGGAATCATCAGTGCTGCTTCCCTCGGGGGACAGACCCAGATCCTGGGGTCCCTCACTACA GCTCCAGTCATTACCAGCGCCATTCCCAGCATGCCAGGGATCAGCAGTCAGATCCTCACCAATGCTCAGG GACAGGTTATTGGAACCCTTCCATGGGTAGTGAACTCAGCTAGTGTGGCGGCCCCAGCACCAGCCCAAAG CCTGCAGGTCCAGGCCGTGACCCCCCAGCTGTTGTTGAACGCCCAGGGCCAGGTGATTGCGACCCTGGCT AGCAGCCCCCTGCCTCCACCTGTGGCTGTCCGGAAGCCAAGCACACCTGAGTCCCCTG CTA AGAGTGAGG TGC AGCCCATCCA GCCCACACCAACCGTGCCCCAGCCTGCTGTGGTCATTGCCAGCCCAGCTCCAGCCGC CAAGCCATCTGCCTCTGCTCCTATCCCAATTACCTGCTCAGAGACCCCCACCGTCAGCCAGTTGGTGTCC AAGCCACATACTCCAAGTCTGGATGAGGATGGGATCAACTTAGAAGAGATCCGGGAGTTTGCCAAGAACT TTAAGATCCGGCGGCTCTCGCTGGGCCTTACACAGACCCAGGTGGGTCAGGCTCTGACTGCAACGGAAGG TCCAGCCTACAGCCAGTCAGCCATCTGCCGGTTCGAGAAGCTAGACATCACACCCAAGAGTGCCCAGAAG CTAAAGCCGGTGCTGGAAAAGTGGCTAAACGAAGCTGAACTGCGGAACCAGGAAGGCCAGCAGAACCTGA TGGAGTTTGTGGGAGGCGAGCCCTCCAAGAAACGCAAACGCCGCACCTCCTTCACCCCCCAGGCCATAGA GGCTCTCAATGCCTATTTTGAGAAGAACCCACTGCCCACAGGCCAGGAGATCACTGAAATTGCTAAGGAG CTCAACTACGACCGTGAGGTAGTGCGGGTCTGGTTCTGCAATCGGCGCCAGACGCTCAAGAACACCAGCA AGCTGAACGTCTTTCAGATCCCTTAGGGCTCAGCCCCTGGCCCTGTGTTCTAGCACTTTGTCCATTTCCC GTGGCATCCGGCTGCAGCCACTGCCATGACAGCACCTGTCATTTTGCCACGTGCAGCTGTGCTCACCCCA GGTCATCAGACTCCACCGTGTGCATGTGCATCAATGTCCCTCTTTTCTCCCACACATCTCACATCATGGG GAGGCCAGAGGGGGCCACACGAGAGCTCCAGGCTCTGGGCTGGTCACTCCGAAGAAGAGGATTTGTGACG TCACTTAGAGAAGCACCTTGCTAGCATGGTTTCTGAAGGGTGAATTCTGGTGGGGAACCAGAAACTCCCT GTCTTTGGGGCAGGGCTAAAGCAGCTCCTAAGGACCACTGGCCATTAGCTCTTGCTTTTGATGGCATTCT CTTTCCACCTTGTCTTCTCCTTTGCTCCTCTGTGTTAGTGTGGCAGGTATGACAACTCATCCAGTGGAAA CACAGCCTCACACTGCCCTTCCGCCCCCCACACTTTGCCTGCAGGTGCACCGAAAGGACCTGGGAGATAA AATTCAAAAAAGTGTGATGTGCTGCTCAGAAGGTCAGACTCCATGTCTGCCTTGACCTCAAGGTCAGAAG GTTCCCAAACCCCTGGGGCTGGAACATGGGATCTCCTCTTCCACCTCTTCCTGGTTCCTTTGCGGGGAAA ATTGCACTAAAACAGAACCTTTTCTTAATCCATGTTGGAAGGAAGCAACAGTGAACTCTACCTGTTCTGG AGTTCTCCTGGGTCTGCAGAAGGTTGGGAATTTAGAAAATAAGGCTGTTCTTTCATATTTTAATTTAATC TCTGTCAATGGCCATCCCTCCCACAAAAAAACGTGGGTTAAGAGAACTTGCAGACTGGATATGCAAGCAA ACGGGCAACTCTGGAGAAAAATAAGGAAAGGAATGCTGACTTTCTCTTTCTTTCTCTTGTCCCCACACCC ATTCCCAACCCAATACTGGGGCCTTCTCAAAAGGAGCAAATTAAACAATAAACCAGACAGCAAGGCCCTG GGGGAAAGGACAACATCCTGAAATAAATGATGGAGCCCAGGAAGGTCTCTTGTGGAAGTTGACTTAACTC TAATTTTCTTTGTAACTTTAAGCCTTGGATACGGGAGGAGAAATCTCATTTTGTCGAGTCTCAGACCATG TCTGTGTGTAAGCAATCCCCACAGTCTCCTCTGAGCCAAGGACACCCCCAGATCAGATTGAGTTTTGCTT CTAGACGGGGTAGCTATGGTACCTTGGGGGTTAGCTCTCATCCAAGCTGTTAAGTGAGTTTCCAGCCTCA CTGTGGCTGGAAAGCCCCTAAAATTCAGTATGTAACTCCAGGAAGTCAGGAGAGAACTGAGATTTGCCTA GATGACCACAGGCTTGCGGTGTAGATTATCCCTAAAGGGCCCCAAGTCACGGGGGTCAACCACCCCTGTC TTCAGTACTCTTATCCTTACAGAGGCTGGTCTCTAACAGCTGCCTCCAGTGGACCTCCCATGATCCACCC TGAGGGAAGGACCGTCAGCTGGGGACACATCACCACCTCTGTCAGTCACTGGTGCAGAGCCACCTCCTAG CCTAGCTTCCTCTGGTGTCCTGTTTCCTTTCCCACTTACTGTTGGTGCCTCCCAGGCCCTGCAGTGCCAG CGTGGCCACCCTCTTGGTAGCCTGGCCAGTAAGAGGAGGACAGTTGTGTGCTGAATTAGCACACGCACGT GCAGCGCGCACAGACGCGCGCACACACACACACATACACGCTCTGCTGCATTTGGACAAACCATGCCTGC CAGAGTGTAGCAGAGGTGAGGAAGCAGGTGGGCAGCTTGCCTGACCCAGCTTTTCAGGAGAGCGTGTCTC CAACAGAGAGTCTCCACACTCTAGTTCAGGGTTATCGACCTGCCTCAATGAGATGACAGACTCATTTGGG AGGGGTGTTGCAAACAAGTTTTCAGTGAGAATAGTTAAGTTCCAGAGCTTGTAAAGGATTCAGTGACTGA CACTTCAGTAAATTAGGCCAGGCACATTGGCTTATGCCTGTAATTCCAACACTTTGGAAGGCCGAGGTGG GCGGATCATTTGAGGTCTGGAGTTCGAGACCAGCCTGACCAACATGGTGAAACCCCGTCTCTACTAAAAA TACAAAAATTAGCCAGGTGTGGTAGTGCACATCTGTAATCCCAGCTACTTGGGAGGTGGAGGCAGGAGAA TTGCTTGAACCCTGGAGGTTGCAATGAGCTGAGATCACACTACTTCACTCCAGCCTGGGTGACAGAGCAA GACTCGGTCTCAAACAAACAAAAACTTATGGCGATGCAGGTTTTCATGCTCAGACGCTTGCATTCAGGTA TGCTTTCTTTTTTGAGAGAGACAAATGGGTCACAGCTGGCACCCTGGGAATAGCACATAATCCAGGGTGT GTCTGTGGTGGTGGACGTGCAGGGGAACACCATCTGTCCTGTCTCATGATGGGAAAACAATCATGAACCA CTGGTCTAAATTAGGCCTGGCCATGCTTTCTCAGCCCCTCCCTCATTTAAATTTGTCTTCCCAAAGCTGA GCTAAAACTAAACCATTTCTCCTCTGCTGGAATGATGGATTGGTCATTCAGAGGAACAATACCAGGGGTG GGAGGTTTGCAGGCTGAGTTCCCCAGGCATGGGGGTGCAGGGTGTCCCTGAGGTTTACCCAAAGCACAGC TCGCTGGCCTGTGACCTCTGCCCTTCCTCCCACAGTGTAAGACCCCCCAGGAAGCAGCTGGGGCCTGAAC CTCTCACCTAGGAGGTAGGTTTATTTTATTTTTTGTTAGCATCAGGCTCTGAAGGAGTTGGTATACATTT TGTTTTGAAAACATCTTCTGGACTTACACCAGAGCTTAGTGTCGTCTTTACTATGGAAAGAGAGGAGAAT GGACAGAAATGGTTTAACTGTGTGGAGTTTTGTTTGTTTTGTTTTAAATGGAAGAAAGACCAAAACTTTC CTGGTGGATCAGCTAGGGCCTTTGACCCTGCATTACCACGGCATTTTATCCAGGTGAAGTCCAGGGAAAG AACTCAGCCAAATGGACTAAGGAACACACGAGTTTGGAATGCGAGACTCTGACATTTTTGTGTTCTTGGA AATCCAATTACCTTCCCATGCCCAGATTTCCTTCCTGCCTCTTGGACCAGGCTCTGGCACTGAGGTTCTC ACTGTTCCCAACACAGACAAAGCTTCCTGAGGGCTGGAGGGGCAGCAAGGGGAGAGGAGAATGGGGAAGA AGCGCTTGATGTAGTTGTGTGGAATAAACAGTATTTTTTCTTTTGTAAAAAAAAAAAAAAAAA SEQ ID NO: 40 - Homo sapiens Ran GTPase activating protein 1 (RANGAP1), mRNA AAATCCTCCTCCTCCGCCATCATCCGCCGCGGTGCGGAGAGCAGGTGGTGCTGGAAGCGCGTGAGGCCGG GAGCTCGAGAGAGCTAACAGACTAGCCGGCTGGACATCTGGACCGCTGGATCCGGAGGTGGCGACCCCGG CCTGACCCGGACCCTAAATCCGTCCCCGCCCCAGAGGGCGGAGGCGCGCGCTCGATTCCCCCCACGCGGC GGCGCCGCCTGTTTACGTCTGCAGATCTCCAGGGGAGCCCACCAGCCTAGTCAACATGGCCTCGGAAGAC ATTGCCAAGCTGGCAGAGACACTTGCCAAGACTCAGGTGGCCGGGGGACAGCTGAGTTTCAAAGGCAAGA GCCTCAAACTCAACACTGCAGAAGATGCTAAAGATGTGATTAAAGAGATTGAAGACTTTGACAGCTTGGA GGCTCTGCGTCTGGAAGGCAACACAGTGGGCGTGGAAGCAGCCAGGGTCATCGCCAAGGCCTTAGAGAAG AAGTCGGAGTTGAAGCGCTGCCACTGGAGTGACATGTTCACGGGAAGGCTGCGGACCGAG ATC CCACCAG CCC TGATCTCACTAG GGGAAGGACTCATCACAGCTGGGGCTCAGCTGGTGGAGCTGGACTTAAGCGACAA CGCATTCGGGCCCGACGGTGTGCAAGGCTTCGAGGCCCTGCTCAAGAGCTCAGCCTGCTTCACCCTGCAG GAACTCAAGCTCAACAACTGTGGCATGGGCATTGGCGGCGGCAAGATCCTGGCTGCAGCTCTGACCGAAT GTCACCGGAAATCCAGTGCCCAAGGCAAGCCTCTGGCCCTGAAGGTCTTTGTGGCTGGCAGAAACCGTCT GGAGAATGATGGCGCCACTGCCTTGGCAGAAGCTTTTAGGGTCATCGGGACCCTGGAGGAGGTCCACATG CCACAGAATGGGATCAACCACCCTGGCATCACTGCCCTGGCCCAGGCTTTCGCTGTCAACCCCCTGCTGC GGGTCATCAACCTGAATGACAACACCTTCACTGAGAAGGGCGCCGTGGCCATGGCCGAGACCTTGAAGAC CTTGCGGCAGGTGGAGGTGATTAATTTTGGGGACTGCCTGGTGCGCTCCAAGGGTGCAGTTGCCATTGCA GATGCCATCCGCGGCGGCCTGCCCAAGCTAAAGGAGCTGAACTTGTCATTCTGTGAAATCAAGAGGGATG CTGCCCTGGCTGTTGCTGAGGCCATGGCAGACAAAGCTGAGCTGGAGAAGCTGGACCTGAATGGCAACAC CCTGGGAGAAGAAGGCTGTGAACAGCTTCAGGAGGTGCTGGAGGGCTTCAACATGGCCAAGGTGCTGGCG TCCCTCAGTGATGACGAGGACGAGGAGGAGGAGGAGGAAGGAGAAGAGGAAGAAGAGGAAGCAGAAGAAG AGGAGGAGGAAGATGAGGAAGAGGAGGAAGAAGAGGAGGAGGAGGAGGAAGAAGAGCCTCAGCAGCGAGG GCAGGGAGAGAAGTCAGCCACGCCCTCACGGAAGATTCTGGACCCTAACACTGGGGAGCCAGCTCCCGTG CTGTCCTCCCCACCTCCTGCAGACGTCTCCACCTTCCTGGCTTTTCCCTCTCCAGAGAAGCTGCTGCGCC TAGGGCCCAAGAGCTCCGTGCTGATAGCCCAGCAGACTGACACGTCTGACCCCGAGAAGGTGGTCTCTGC CTTCCTAAAGGTGTCATCTGTGTTCAAGGACGAAGCTACTGTGAGGATGGCAGTGCAGGATGCAGTAGAT GCCCTGATGCAGAAGGCTTTCAACTCCTCGTCCTTCAACTCCAACACCTTCCTCACCAGGCTGCTCGTGC ACATGGGTCTGCTCAAGAGTGAAGACAAGGTCAAGGCCATTGCCAACCTGTACGGCCCCCTGATGGCGCT GAACCACATGGTGCAGCAGGACTATTTCCCCAAGGCCCTTGCACCCCTGCTGCTGGCGTTCGTGACCAAG CCCAACAGCGCCCTGGAATCCTGCTCCTTCGCCCGCCACAGTCTGCTGCAGACGCTGTACAAGGTCTAGA CTCAAAGCCTCTCCCATCCCTTGGCCTGGACCAGTGAGCTGGGGAGGGACTCGGATGAACTGAGGCGCAG CCTACGCCATTGCCTTGGACAGGACTCTGGCCACAGGCAGGGCGGGTCTGTGTCCCATGTGTCCTGTCAG TCCCCTGAGTATGTGTGTGGGTGTGGCGCATGTGCAGGTCTGTGCCTCCTGTCGGGATTTGGGTTTTAAC GTCTTCTGCTGGCCCAGCCCTGCTCTGTTGTGGGGAGTTGGCCCCCAGGGGAAAGGGCTGTGAGCTGCTC CGCCATTAAACTCACCTCCACCTGAGGGCGCTCTGCTGATCTCCGCCTGGGCCCTGATGGCCGTCCCCAC CCACCTGCCTTCCGGCCCGGCTCCCTGGCGGAGCCAGAACCCAGGGAGTTGCCCGCGTGCTGTCCTTCCC CTCTGTGTTGTGATTGGGTTGTTTCCTGCCCTGCCTGGGGCTGCTTCTCGTCACCAAGCCCTGGTCCTGC GGCAGCTGTCACCCCTACCATCCATACCACTGTGCTGACCGCTCAGCCTGAAGAGCAGAGAATGCCATGG GTGGGACTGTGGGGGTCGGATCGTGGGGTTGTTGGCAGAGGGCAACCCTGGGCCCCACACCGTGTGGACA GGCAGACACCAGATTGTCCAGGAGCAGGAGCTGCTGGGACTGCGCTGGCCCCGGACCTAGTGGGCCTTCT CCTGGCTGCTGAGATGTCGTCTGTGACTGGCCTGGCTGGAGGGGGAGTGTTGACAACCCAAAGCTGTTCT CCAGTCTGGGGAGGGAGAGGCAGGGTCCCCAATGTCCGAGCTGCATCTGGACGCTGCTCTTAAAGGACCT CCTGGGGCAGGGGAGCGGTAGGGTCTGGACTGGGCAGATGCTGTATGACCTCCCTGAGCACCCGTGACTG CCCCATGCTTTCCCCTTTGTGCTCTGTGTGTGTCTGGGCTGTGCCCGGGGGCTTCACAAATAAAGTCGTG TGGCAGCTTCAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 41 - Homo sapiens Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), mRNA GGCAAACAGCCCGCCCGGCACCACCATGCTCGCCCTGGAGGCTGCACAGCTCGACGGG CCA CACTTCAGC TGT CTGTACCCAG ATGGCGTCTTCTATGACCTGGACAGCTGCAAGCATTCCAGCTACCCTGATTCAGAGG GGGCTCCTGACTCCCTGTGGGACTGGACTGTGGCCCCACCTGTCCCAGCCACCCCCTATGAAGCCTTCGA CCCGGCAGCAGCCGCTTTTAGCCACCCCCAGGCTGCCCAGCTCTGCTACGAACCCCCCACCTACAGCCCT GCAGGGAACCTCGAACTGGCCCCCAGCCTGGAGGCCCCGGGGCCTGGCCTCCCCGCATACCCCACGGAGA ACTTCGCTAGCCAGACCCTGGTTCCCCCGGCATATGCCCCGTACCCCAGCCCTGTGCTATCAGAGGAGGA AGACTTACCGTTGGACAGCCCTGCCCTGGAGGTCTCGGACAGCGAGTCGGATGAGGCCCTCGTGGCTGGC CCCGAGGGGAAGGGATCCGAGGCAGGGACTCGCAAGAAGCTGCGCCTGTACCAGTTCCTGCTGGGGCTAC TGACGCGCGGGGACATGCGTGAGTGCGTGTGGTGGGTGGAGCCAGGCGCCGGCGTCTTCCAGTTCTCCTC CAAGCACAAGGAACTCCTGGCGCGCCGCTGGGGCCAGCAGAAGGGGAACCGCAAGCGCATGACCTACCAG AAGCTGGCGCGCGCCCTCCGAAACTACGCCAAGACCGGCGAGATCCGCAAGGTCAAGCGCAAGCTCACCT ACCAGTTCGACAGCGCGCTGCTGCCTGCAGTCCGCCGGGCCTGAGCACACCCGAGGCTCCCACCTGCGGA GCCGCTGGGGGACCTCACGTCCCAGCCAGGATCCCCCTGGAAGAAAAAGGGCGTCCCCACACTCTAGGTG ATAGGACTTACGCATCCCCACCTTTTGGGGTAAGGGGAGTGCTGCCCTGCCATAATCCCCAAGCCCAGCC CGGGCCTGTCTGGGATTCCCCACTTGTGCCTGGGGTCCCTCTGGGATTTCTTTGTCATGTACAGACTCCC TGGGATCCTCATGTTTTGGGTGACAGGACCTATGGACCACTATACTCGGGGAGGCAGGGTAGCAGTTCTT CCAGAATCCCAAGAGCTTCTCTGGGATTTTCTTGTGATATCTGATTCCCCAGTGAGGCCTGGGACGTTTT TAAGATCGCTGTGTGTCTGTAAACCCTGAATCTCATCTGGGGTGGGGGCCCTGCTGGCAACCCTGAGCCC TGTCCAAGGTTCCCTCTTGTCAGATCTGAGATTTCCTAGTTATGTCTGGGGCCCTCTGGGAGCTGTTATC ATCTCAGATCTCTTCGCCCATCTATGGCTGTGTTGTCACATCTGTCCCCTCATTTTTGAGATCCCCCAAT TCTCTGGAACTATTCTGCTGCCCCTTTTTATGTGTCTGGAGTTCCCCAATCACATCTAGGGCTCCTCCAA GAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 42 - Homo sapiens TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28 kDa (TAF11), mRNA AAGATCCTGGCCTGTGCAGCTCGGGTTTCCGAGCTTCTGCCTCAGGCATCTCCGCGATCTCCTCTCCCCT CCAATCCTATCCGTGATGGACGATGCCCACGAGTCGCCCTCCGACAAAGGTGGAGAGACAGGGGAGTCGG ATGAGACGGCCCCTGTGCCCGGGGACCCGGGGGCTACCGACACCGATGGAATCCCAGAGGAAACTGACGG AGACGCAGATGTGGACTTGAAAGAAGCTGCAGCGGAG GAA GGCGAGCTCGAGAGTCAGGATG TCTCAGAT TTAACAACAGTTGAAAGGGAAGACTCATCATTACTTAATCCTGCAGCCAAAAAACTGAAAATAGATACCA AAGAAAAGAAAGAGAAAAAGCAGAAAGTAGATGAAGATGAGATTCAGAAGATGCAAATCCTGGTTTCTTC TTTTTCTGAGGAGCAGCTGAACCGTTATGAAATGTATCGCCGCTCAGCTTTCCCTAAGGCAGCCATCAAA AGGCTGATCCAGTCCATCACTGGCACCTCTGTGTCTCAGAATGTTGTTATTGCTATGTCTGGTATTTCCA AGGTTTTCGTCGGGGAGGTGGTAGAAGAAGCACTGGATGTGTGTGAGAAGTGGGGAGAAATGCCACCACT ACAACCCAAACATATGAGGGAAGCCGTTAGAAGGTTAAAGTCAAAAGGACAGATCCCTAACTCGAAGCAC AAAAAAATCATCTTCTTCTAGACCAAAGTCTAGAAAGGCCTATGTTACTGACGGAAGAAGTATTGGTTCC AGACTTCCTATAAGACTGTCTGCATTGGTGCTTTAGTATCTCAGGCCTCCAAGGATTCCATGATGATTTT AATGTCTTTCTCAAAACTCTGATATTTGTCACACCTAGAAAGTATGTAGCCTGATTGATACTTGCCTTGA CTAAATTTTGGGACCTCTTGGGGCATTTTGAAGTATTTAACTGTCTTGACCAGTTGGAAGAAGATACGTG GGCCATAAGCATCTTCTGGACAGGGGAACTGCTTTCAGAGAGAAAACCTTTCCAAGAGAGTTTTGTTTTG TTTTGGTTTCGTTTTGTTTGAGATAGGGTCTTGCTCTATCACCTAGGCTGGAGTGCAGCGGCATGACTGC AGCCTTGAACTCCTGGGCTTAAGTGACCCTCCCACCTCAGTCTCCTGAGTAGCTAGGACTACAGGCACAC ACTACTGTGCCCAGCTAACTTATTTTTATTTTTTATGGAGATGGGGTCTTGCTTTGTTGCCCAGGCTGGT CGTGAACTCCTGGCTTCAAGCAGTCCTCCTGCCTCAGCCTCCTAAAGTGCCGAGGGCTTTAATGGTTTCA CATTGAAGCCTGAAGTTGCTAAGACTTAGGTTGTTTCTTATATCTGGTTTTAAGTAGATGAAACAACCAG AAACTTTTACTTGTGATACTCTACCATGAAGGATGCGGTAATGGCAGGAATAGCAGAATAATTGGTGCTT GTAAACATTTAAGATTCTCCTGTGGATTTTGGTGAGTGATCATTAAACTGTTTTCCAACTTGCAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO: 43 - Homo sapiens TATA box binding protein (TBP), transcript variant 2, mRNA GGCGGAAGTGACATTATCAACGCGCGCCAGGGGTTCAGTGAGGTCGGGCAGGTTCGCTGTGGCGGGCGCC TGGGCCGCCGGCTGTTTAACTTCGCTTCCGCTGGCCCATAGTGATCTTTGCAGTGACCCAGGGTGCCATG ACTCCCGGAATCCCTATCTTTAGTCCAATGATGCCTTATGGCACTGGACTGACCCCACAGCCTATTCAGA ACACCAATAGTCTGTCTATTTTGGAAGAGCAACAAAGGCAGCAGCAGCAACAACAACAGCAGCAGCAGCA GCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG CAGCAGCAACAGGCAGTGGCAGCTGCAGCCGTTCAGCAGTCAACGTCCCAGCAGGCAACACAGGGAACCT CAGGCCAGGCACCACAGCTCTTCCACTCACAGACTCTCACAACTGCACCCTTGCCGGGCACCACTCCACT GTATCCCTCCCCCATGACTCCCATGACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATT GTACC GCA GCTGCAAAATATTGTATCCACA GTGAATCTTGGTTGTAAACTTGACCTAAAGACCATTGCAC TTCGTGCCCGAAACGCCGAATATAATCCCAAGCGGTTTGCTGCGGTAATCATGAGGATAAGAGAGCCACG AACCACGGCACTGATTTTCAGTTCTGGGAAAATGGTGTGCACAGGAGCCAAGAGTGAAGAACAGTCCAGA CTGGCAGCAAGAAAATATGCTAGAGTTGTACAGAAGTTGGGTTTTCCAGCTAAGTTCTTGGACTTCAAGA TTCAGAATATGGTGGGGAGCTGTGATGTGAAGTTTCCTATAAGGTTAGAAGGCCTTGTGCTCACCCACCA ACAATTTAGTAGTTATGAGCCAGAGTTATTTCCTGGTTTAATCTACAGAATGATCAAACCCAGAATTGTT CTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAACAGGTGCTAAAGTCAGAGCAGAAATTTATGAAGCAT TTGAAAACATCTACCCTATTCTAAAGGGATTCAGGAAGACGACGTAATGGCTCTCATGTACCCTTGCCTC CCCCACCCCCTTCTTTTTTTTTTTTTAAACAAATCAGTTTGTTTTGGTACCTTTAAATGGTGGTGTTGTG AGAAGATGGATGTTGAGTTGCAGGGTGTGGCACCAGGTGATGCCCTTCTGTAAGTGCCCACCGCGGGATG CCGGGAAGGGGCATTATTTGTGCACTGAGAACACCGCGCAGCGTGACTGTGAGTTGCTCATACCGTGCTG CTATCTGGGCAGCGCTGCCCATTTATTTATATGTAGATTTTAAACACTGCTGTTGACAAGTTGGTTTGAG GGAGAAAACTTTAAGTGTTAAAGCCACCTCTATAATTGATTGGACTTTTTAATTTTAATGTTTTTCCCCA TGAACCACAGTTTTTATATTTCTACCAGAAAAGTAAAAATCTTTTTTAAAAGTGTTGTTTTTCTAATTTA TAACTCCTAGGGGTTATTTCTGTGCCAGACACATTCCACCTCTCCAGTATTGCAGGACAGAATATATGTG TTAATGAAAATGAATGGCTGTACATATTTTTTTCTTTCTTCAGAGTACTCTGTACAATAAATGCAGTTTA TAAAAGTGTTAGATTGTTGTTAAAAAAAAAAAAAAAAAA SEQ ID NO: 44 - Homo sapiens transforming growth factor, beta receptor II (70/80 kDa) (TGFBR2), transcript variant 1, mRNA GGAGAGGGAGAAGGCTCTCGGGCGGAGAGAGGTCCTGCCCAGCTGTTGGCGAGGAGTTTCCTGTTTCCCC CGCAGCGCTGAGTTGAAGTTGAGTGAGTCACTCGCGCGCACGGAGCGACGACACCCCCGCGCGTGCACCC GCTCGGGACAGGAGCCGGACTCCTGTGCAGCTTCCCTCGGCCGCCGGGGGCCTCCCCGCGCCTCGCCGGC CTCCAGGCCCCCTCCTGGCTGGCGAGCGGGCGCCACATCTGGCCCGCACATCTGCGCTGCCGGCCCGGCG CGGGGTCCGGAGAGGGCGCGGCGCGGAGGCGCAGCCAGGGGTCCGGGAAGGCGCCGTCCGCTGCGCTGGG GGCTCGGTCTATGACGAGCAGCGGGGTCTGCCATGGGTCGGGGGCTGCTCAGGGGCCTGTGGCCGCTGCA CATCGTCCTGTGGACGCGTATCGCCAGCACGATCCCACCGCACGTTCAGAAGTCGGATGTGGAAATGGAG GCCCAGAAAGATGAAATCATCTGCCCCAGCTGTAATAGGACTGCCCATCCACTGAGACATATTAATAACG ACATGATAGTCACTGACAACAACGGTGCAGTCAAGTTTCCACAACTGTGTAAATTTTGTGATGTGAGATT TTCCACCTGTGACAACCAGAAATCCTGCATGAGCAACTGCAGCATCACCTCCATCTCTGAGAAGCCACAG GAAGTCTGTGTGGCTGTATGGAGAAAGAATGACGAGAACATAACACTAGAGACAGTTTGCCATGACCCCA AGCTCCCCTACCATGACTTTATTCTGGAAGATGCTGCTTCTCCAAAGTGCATTATGAAGGAAAAAAAAAA GCCTGGTGAGACTTTCTTCATGTGTTCCTGTAGCTCTGATGAGTGCAATGACAACATCATCTTCTCAGAA GAATATAACACCAGCAATCCTGACTTGTTGCTAGTCATATTTCAAGTGACAGGCATCAGCCTCCTGCCAC CACTGGGAGTTGCCATATCTGTCATCATCATCTTCTACTGCTACCGCGTTAACCGGCAGCAGAAGCTGAG TTCAACCTGGGAAACCGGCAAGACGCGGAAGCTCATGGAGTTCAGCGAGCACTGTGCCATCATCCTGGAA GATGACCGCTCTGACATCAGCTCCACGTGTGCCAACAACATCAACCACAACACAGAGCTGCTGCCCATTG AGCTGGACACCCTGGTGGGGAAAGGTCGCTTTGCTGAGGTCTATAAGGCCAAGCTGAAGCAGAACACTTC AGAGCAGTTTGAGACAGTGGCAGTCAAGATCTTTCCCTATGAGGAGTATGCCTCTTGGAAGACAGAGAAG GACATCTTCTCAGACATCAATCTGAAGCATGAGAACATACTCCAGTTCCTGACGGCTGAGGAGCGGAAGA CGGAGTTGGGGAAACAATACTGGCTGATCACCGCCTTCCACGCCAAGGGCAACCTACAGGAGTACCTGAC GCGGCATGTCATCAGCTGGGAGGACCTGCGCAAGCTGGGCAGCTCCCTCGCCCGGGGGATTGCTCACCTC CACAGTGATCACACTCCATGTGGGAGGCCCAAGATGCCCATCGTGCACAGGGACCTCAAGAGCTCCAATA TCCTCGTGAAGAACGACCTAACCTGCTGCCTGTGTGACTTTGGGCTTTCCCTGCGTCTGGACCCTACTCT GTCTGTGGATGACCTGGCTAACAGTGGGCAGGTGGGAACTGCAAGATACATGGCTCCAGAAGTCCTAGAA TCCAGGATGAATTTGGAGAATGTTGAGTCCTTCAAGCAGACCGATGTCTACTCCATGGCTCTGGTGCTCT GGGAAATGACATCTCGCTGTAATGCAGTGGGAGAAGTAAAAGATTATGAGCCTCCATTTGGTTCCAAGGT GCGGGAGCACCCCTGTGTCGAAAGCATGAAGGACAACGTGTTGAGAGATCGAGGGCGACCAGAAATTCCC AGCTTCTGG CTC AACCACCAGGGCATCCAGATGG TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACC CAGAGGCCCGTCTCACAGCCCAGTGTGTGGCAGAACGCTTCAGTGAGCTGGAGCATCTGGACAGGCTCTC GGGGAGGAGCTGCTCGGAGGAGAAGATTCCTGAAGACGGCTCCCTAAACACTACCAAATAGCTCTTCTGG GGCAGGCTGGGCCATGTCCAAAGAGGCTGCCCCTCTCACCAAAGAACAGAGGCAGCAGGAAGCTGCCCCT GAACTGATGCTTCCTGGAAAACCAAGGGGGTCACTCCCCTCCCTGTAAGCTGTGGGGATAAGCAGAAACA ACAGCAGCAGGGAGTGGGTGACATAGAGCATTCTATGCCTTTGACATTGTCATAGGATAAGCTGTGTTAG CACTTCCTCAGGAAATGAGATTGATTTTTACAATAGCCAATAACATTTGCACTTTATTAATGCCTGTATA TAAATATGAATAGCTATGTTTTATATATATATATATATATCTATATATGTCTATAGCTCTATATATATAG CCATACCTTGAAAAGAGACAAGGAAAAACATCAAATATTCCCAGGAAATTGGTTTTATTGGAGAACTCCA GAACCAAGCAGAGAAGGAAGGGACCCATGACAGCATTAGCATTTGACAATCACACATGCAGTGGTTCTCT GACTGTAAAACAGTGAACTTTGCATGAGGAAAGAGGCTCCATGTCTCACAGCCAGCTATGACCACATTGC ACTTGCTTTTGCAAAATAATCATTCCCTGCCTAGCACTTCTCTTCTGGCCATGGAACTAAGTACAGTGGC ACTGTTTGAGGACCAGTGTTCCCGGGGTTCCTGTGTGCCCTTATTTCTCCTGGACTTTTCATTTAAGCTC CAAGCCCCAAATCTGGGGGGCTAGTTTAGAAACTCTCCCTCAACCTAGTTTAGAAACTCTACCCCATCTT TAATACCTTGAATGTTTTGAACCCCACTTTTTACCTTCATGGGTTGCAGAAAAATCAGAACAGATGTCCC CATCCATGCGATTGCCCCACCATCTACTAATGAAAAATTGTTCTTTTTTTCATCTTTCCCCTGCACTTAT GTTACTATTCTCTGCTCCCAGCCTTCATCCTTTTCTAAAAAGGAGCAAATTCTCACTCTAGGCTTTATCG TGTTTACTTTTTCATTACACTTGACTTGATTTTCTAGTTTTCTATACAAACACCAATGGGTTCCATCTTT CTGGGCTCCTGATTGCTCAAGCACAGTTTGGCCTGATGAAGAGGATTTCAACTACACAATACTATCATTG TCAGGACTATGACCTCAGGCACTCTAAACATATGTTTTGTTTGGTCAGCACAGCGTTTCAAAAAGTGAAG CCACTTTATAAATATTTGGAGATTTTGCAGGAAAATCTGGATCCCCAGGTAAGGATAGCAGATGGTTTTC AGTTATCTCCAGTCCACGTTCACAAAATGTGAAGGTGTGGAGACACTTACAAAGCTGCCTCACTTCTCAC TGTAAACATTAGCTCTTTCCACTGCCTACCTGGACCCCAGTCTAGGAATTAAATCTGCACCTAACCAAGG TCCCTTGTAAGAAATGTCCATTCAAGCAGTCATTCTCTGGGTATATAATATGATTTTGACTACCTTATCT GGTGTTAAGATTTGAAGTTGGCCTTTTATTGGACTAAAGGGGAACTCCTTTAAGGGTCTCAGTTAGCCCA AGTTTCTTTTGCTTATATGTTAATAGTTTTACCCTCTGCATTGGAGAGAGGAGTGCTTTACTCCAAGAAG CTTTCCTCATGGTTACCGTTCTCTCCATCATGCCAGCCTTCTCAACCTTTGCAGAAATTACTAGAGAGGA TTTGAATGTGGGACACAAAGGTCCCATTTGCAGTTAGAAAATTTGTGTCCACAAGGACAAGAACAAAGTA TGAGCTTTAAAACTCCATAGGAAACTTGTTAATCAACAAAGAAGTGTTAATGCTGCAAGTAATCTCTTTT TTAAAACTTTTTGAAGCTACTTATTTTCAGCCAAATAGGAATATTAGAGAGGGACTGGTAGTGAGAATAT CAGCTCTGTTTGGATGGTGGAAGGTCTCATTTTATTGAGATTTTTAAGATACATGCAAAGGTTTGGAAAT AGAACCTCTAGGCACCCTCCTCAGTGTGGGTGGGCTGAGAGTTAAAGACAGTGTGGCTGCAGTAGCATAG AGGCGCCTAGAAATTCCACTTGCACCGTAGGGCATGCTGATACCATCCCAATAGCTGTTGCCCATTGACC TCTAGTGGTGAGTTTCTAGAATACTGGTCCATTCATGAGATATTCAAGATTCAAGAGTATTCTCACTTCT GGGTTATCAGCATAAACTGGAATGTAGTGTCAGAGGATACTGTGGCTTGTTTTGTTTATGTTTTTTTTTC TTATTCAAGAAAAAAGACCAAGGAATAACATTCTGTAGTTCCTAAAAATACTGACTTTTTTCACTACTAT ACATAAAGGGAAAGTTTTATTCTTTTATGGAACACTTCAGCTGTACTCATGTATTAAAATAGGAATGTGA ATGCTATATACTCTTTTTATATCAAAAGTCTCAAGCACTTATTTTTATTCTATGCATTGTTTGTCTTTTA CATAAATAAAATGTTTATTAGATTGAATAAAGCAAAATACTCAGGTGAGCATCCTGCCTCCTGTTCCCAT TCCTAGTAGCTAAA SEQ ID NO: 45 - Homo sapiens tumor protein p53 (TP53), transcript variant 4, mRNA GATTGGGGTTTTCCCCTCCCATGTGCTCAAGACTGGCGCTAAAAGTTTTGAGCTTCTCAAAAGTCTAGAG CCACCGTCCAGGGAGCAGGTAGCTGCTGGGCTCCGGGGACACTTTGCGTTCGGGCTGGGAGCGTGCTTTC CACGACGGTGACACG CTT CCCTGGATTGGCAGCCAGACTG CCTTCCGGGTCACTGCCATGGAGGAGCCGC AGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCAGACCTATGGAAACTACTTCCTGA AAACAACGTTCTGTCCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAA CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCC CTGCACCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCC TTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCT GTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGC TGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATGGCCATCTACAAGCAGTCACAGCA CATGACGGAGGTTGTGAGGCGCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTCTGGCCCCTCCT CAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGACAGAAACACTTTTCGACATA GTGTGGTGGTGCCCTATGAGCCGCCTGAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTG TAACAGTTCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACCATCATCACACTGGAAGACTCCAGT GGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGGCGCACAG AGGAAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACTAAGCGAGCACT GCCCAACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCAG ATGCTACTTGACTTACGATGGTGTTACTTCCTGATAAACTCGTCGTAAGTTGAAAATATTATCCGTGGGC GTGAGCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATGCCCAGGCTGGGAAGGA GCCAGGGGGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAA AAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGACATTCTCCACTTCTTGTTCCCCACTGACAGC CTCCCACCCCCATCTCTCCCTCCCCTGCCATTTTGGGTTTTGGGTCTTTGAACCCTTGCTTGCAATAGGT GTGCGTCAGAAGCACCCAGGACTTCCATTTGCTTTGTCCCGGGGCTCCACTGAACAAGTTGGCCTGCACT GGTGTTTTGTTGTGGGGAGGAGGATGGGGAGTAGGACATACCAGCTTAGATTTTAAGGTTTTTACTGTGA GGGATGTTTGGGAGATGTAAGAAATGTTCTTGCAGTTAAGGGTTAGTTTACAATCAGCCACATTCTAGGT AGGGGCCCACTTCACCGTACTAACCAGGGAAGCTGTCCCTCACTGTTGAATTTTCTCTAACTTCAAGGCC CATATCTGTGAAATGCTGGCATTTGCACCTACCTCACAGAGTGCATTGTGAGGGTTAATGAAATAATGTA CATCTGGCCTTGAAACCACCTTTTATTACATGGGGTCTAGAACTTGACCCCCTTGAGGGTGCTTGTTCCC TCTCCCTGTTGGTCGGTGGGTTGGTAGTTTCTACAGTTGGGCAGCTGGTTAGGTAGAGGGAGTTGTCAAG TCTCTGCTGGCCCAGCCAAACCCTGTCTGACAACCTCTTGGTGAACCTTAGTACCTAAAAGGAAATCTCA CCCCATCCCACACCCTGGAGGATTTCATCTCTTGTATATGATGATCTGGATCCACCAAGACTTGTTTTAT GCTCAGGGTCAATTTCTTTTTTCTTTTTTTTTTTTTTTTTTCTTTTTCTTTGAGACTGGGTCTCGCTTTG TTGCCCAGGCTGGAGTGGAGTGGCGTGATCTTGGCTTACTGCAGCCTTTGCCTCCCCGGCTCGAGCAGTC CTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTCATGCCACCATGGCCAGCCAACTTTTGCATGTTT TGTAGAGATGGGGTCTCACAGTGTTGCCCAGGCTGGTCTCAAACTCCTGGGCTCAGGCGATCCACCTGTC TCAGCCTCCCAGAGTGCTGGGATTACAATTGTGAGCCACCACGTCCAGCTGGAAGGGTCAACATCTTTTA CATTCTGCAAGCACATCTGCATTTTCACCCCACCCTTCCCCTCCTTCTCCCTTTTTATATCCCATTTTTA TATCGATCTCTTATTTTACAATAAAACTTTGCTGCCACCTGTGTGTCTGAGGGGTG SEQ ID NO: 46 - Homo sapiens tumor protein p53 (TP53), transcript variant 2, mRNA GATTGGGGTTTTCCCCTCCCATGTGCTCAAGACTGGCGCTAAAAGTTTTGAGCTTCTCAAAAGTCTAGAG CCACCGTCCAGGGAGCAGGTAGCTGCTGGGCTCCGGGGACACTTTGCGTTCGGGCTGGGAGCGTGCTTTC CACGACGGTGACACGCTTCCCTGGATTGGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGT CAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCAGACCTATGGAAACTACTTCCTGAAAA CAACGTTCTGTCCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAA TGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTG CACCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTC CCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAG TCT GTG ACT TGCACGTACTCCCCTG CCCTCAACAAGATGTTTTGCCAACTGGCCAAGACCTGCCCTGTGCAGCTGT GGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATGGCCATCTACAAGCAGTCACAGCACAT GACGGAGGTTGTGAGGCGCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTCTGGCCCCTCCTCAG CATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGACAGAAACACTTTTCGACATAGTG TGGTGGTGCCCTATGAGCCGCCTGAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAA CAGTTCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACCATCATCACACTGGAAGACTCCAGTGGT AATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGG AAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACTAAGCGAGCACTGCC CAACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAATATTTCACCCTTCAGATC CGTGGGCGTGAGCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATGCCCAGGCTG GGAAGGAGCCAGGGGGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCG CCATAAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGACATTCTCCACTTCTTGTTCCCCAC TGACAGCCTCCCACCCCCATCTCTCCCTCCCCTGCCATTTTGGGTTTTGGGTCTTTGAACCCTTGCTTGC AATAGGTGTGCGTCAGAAGCACCCAGGACTTCCATTTGCTTTGTCCCGGGGCTCCACTGAACAAGTTGGC CTGCACTGGTGTTTTGTTGTGGGGAGGAGGATGGGGAGTAGGACATACCAGCTTAGATTTTAAGGTTTTT ACTGTGAGGGATGTTTGGGAGATGTAAGAAATGTTCTTGCAGTTAAGGGTTAGTTTACAATCAGCCACAT TCTAGGTAGGGGCCCACTTCACCGTACTAACCAGGGAAGCTGTCCCTCACTGTTGAATTTTCTCTAACTT CAAGGCCCATATCTGTGAAATGCTGGCATTTGCACCTACCTCACAGAGTGCATTGTGAGGGTTAATGAAA TAATGTACATCTGGCCTTGAAACCACCTTTTATTACATGGGGTCTAGAACTTGACCCCCTTGAGGGTGCT TGTTCCCTCTCCCTGTTGGTCGGTGGGTTGGTAGTTTCTACAGTTGGGCAGCTGGTTAGGTAGAGGGAGT TGTCAAGTCTCTGCTGGCCCAGCCAAACCCTGTCTGACAACCTCTTGGTGAACCTTAGTACCTAAAAGGA AATCTCACCCCATCCCACACCCTGGAGGATTTCATCTCTTGTATATGATGATCTGGATCCACCAAGACTT GTTTTATGCTCAGGGTCAATTTCTTTTTTCTTTTTTTTTTTTTTTTTTCTTTTTCTTTGAGACTGGGTCT CGCTTTGTTGCCCAGGCTGGAGTGGAGTGGCGTGATCTTGGCTTACTGCAGCCTTTGCCTCCCCGGCTCG AGCAGTCCTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTCATGCCACCATGGCCAGCCAACTTTTG CATGTTTTGTAGAGATGGGGTCTCACAGTGTTGCCCAGGCTGGTCTCAAACTCCTGGGCTCAGGCGATCC ACCTGTCTCAGCCTCCCAGAGTGCTGGGATTACAATTGTGAGCCACCACGTCCAGCTGGAAGGGTCAACA TCTTTTACATTCTGCAAGCACATCTGCATTTTCACCCCACCCTTCCCCTCCTTCTCCCTTTTTATATCCC ATTTTTATATCGATCTCTTATTTTACAATAAAACTTTGCTGCCACCTGTGTGTCTGAGGGGTG SEQ ID NO: 47 - Homo sapiens TXK tyrosine kinase (TXK), mRNA GATTTCAGTTGAAAGATGTGTTTTTGTGAGTAGAGCACCGCAGAAGAACTGAAGACTGTTGTGTGCTCCC CGCAGAAGGGGCTACCATGATCCTTTCCTCCTATAACACCATCCAGTCGGTTTTCTGTTGCTGCTGTTGC TGTTCAGTGCAGAAGCGACAAATGAGAACACAGATAAGCCTGAGCACAGATGAAGAGCTTCCAGAAAAAT ACACCCAGCGTCGCAGGCCGTGGCTCAGCCAATTGTCAAATAAGAAGCAATCCAACACGGGCCGTGTGCA GCCGTCAAAACGAAAGCCACTGCCTCCCCTCCCACCCTCTGAGGTTGCTGAAGAGAAGATCCAAGTCAAG GCACTTTATGATTTTCTGCCCAGAGAACCCTGTAATTTAGCCTTAAGGAGAGCAGAAGAATACCTGATAC TGGAGAAATACAATCCTCACTGGTGGAAGGCAAGAGACCGTTTGGGGAATGAAGGCTTAATCCCAAGCAA CTATGTGACTGAAAACAAAATAACTAATTTAGAAATATATGAGTGGTACCATAGAAACATTACCAGAAAT CAGGCAGAACATCTATTGAGACAAGAGTCTAAAGAAGGTGCATTTATTGTCAGAGATTCAAGACATTTAG GATCCTACACAATTTCCGTATTTATGGGAGCTAGAAGAAGTACGGAGGCTGCCATAAAACATTATCAGAT AAAAAAGAATGACTCAGGACAGTGGTATGTGGCTGAAAGACACGCCTTTCAATCAATCCCTGAGTTAATC TGGTATCACCAGCACAATGCAGCCGGTCTCATGACTCGTCTCCGATATCCAGTTGGGCTGATGGGCAGTT GTTTACCAGCCACAGCTGGGTTTAGCTACGAAAAGTGGGAGATAGATCCATCTGAGTTGGCTTTTATAAA GGAGATTGGAAGCGGTCAGTTTGGAGTGGTCCATTTAGGTGAATGGCGGTCACATATCCAGGTAGCTATC AAGGCCATCAATGAAGGCTCCATGTCTGAAGAGGATTTCATTGAAGAGGCCAAAGTGATGATGAAATTAT CTCATTCAAAGCTAGTGCAACTTTATGGAGTCTGTATACAGCGGAAGCCCCTTTACATTGTGACAGAGTT CATGGAAAATGGCTGCCTGCTTAACTATCTCAGGGAGAATAAAGGAAAGCTTAGGAAGGAAATGCTACTG AGTGTATGCCAGGATATATGTGAAGGAATGGAATATCTGGAGAGGAATGGCTATATTCATAG GGATTTGG CGG CAAGGAATTGTTTG GTCAGTTCAACATGCATAGTAAAAATTTCAGACTTTGGAATGACAAGGTACGT TTTGGATGATGAGTATGTCAGTTCTTTTGGAGCCAAGTTCCCAATCAAGTGGTCCCCTCCTGAAGTTTTT CTTTTCAATAAGTACAGCAGTAAATCTGATGTCTGGTCATTTGGAGTTTTAATGTGGGAAGTTTTTACAG AAGGAAAAATGCCTTTTGAAAATAAGTCAAATTTGCAAGTCGTGGAAGCTATTTCTGAAGGCTTCAGGCT ATATCGCCCTCACCTGGCACCAATGTCCATATATGAAGTCATGTACAGCTGCTGGCATGAGAAACCTGAA GGCCGCCCTACATTTGCCGAGCTGCTGCGGGCTGTCACAGAGATTGCGGAAACCTGGTGACCGGAAACAG AATGCCAACCCAAAGAGTCATCTTGCAAAACTGTCATTTATTGTGAATATCTTCACCATATGGGGTCACT TATGGTGAATATCTTTCTTCAGAGTTGCTGACTCTTGAAAACAGTGCAAAGATCACAGTTTTTAAAAGTT TTAAAAATTTAAGAATATTCACACAATCGTTTTTCTATGTGTGAGAGGGATTTGCACACTCTTATTTTTC TGTAAAATATTTCACATCCCAAATGTGAAGAAGTGAAAAAGACTTCGCAGCAGTCTTCATTGTGGTGCTC TTCATGATCATAGCCCCAGGAACCCTTGAGGTTCTTCTTCACAAGGCTGAGAGTGCTTCCTTCTTGAAGA CGAGTGACATTCATCACTTCAGTGATCCATGCATAGAATATGAAAATAAATTCTTCCAACTCATGGGATA AAGGGGACTCCCTTGAAGAATTTCATGTTTTTGGGCTGTATAGCTCTTTACAGAAAATGCACCTTTATAA ATCACATGAATGTTAGTATTCTGGAAATGTCTTTTGTTAATATAATCTTCCCATGTTATTTAACAAATTG TTTTTGCACATATCTGATTATATTGAAAGCAGTTTTTTGCATTCGAGTTTTAAACACTGTTATAAAATGT AGCCAAAGCTCACCTTTGAACAGATCCCGGTGACATTCTATTTCCAGGAAAATCCGGAACCTGATTTTAG TTCTGTGATTTTACACTTTTTACATGTGAGATTGGACAGTTTCAGAGGCCTTATTTTGTCATACTAAGTG TCTCCTGTAATTTTCAGGAAGATGATTTGTTCTTTCCAGAAGAGGAGACAAAAGCAAGATAGCCAAATGT GACATCAAGCTCCATTGTTTCGGAAATCCAGGATTTTGAATTCGAGATGAAACAACCAGCAATCACAGTT AAATCTTAACTTTGCCTGCACTCTTTGTAGGAATGATCAGAAATTTATCTTTATCATTCTGAGTGCTTCA GGAGTACAATAGGAAGAAAGATACTGGAGAAAGCACTAATGTAATCACCATGAAGTCTGACAACAGGAGC CCATTATTTGCGTACTGTCCCACCCTGTATCATGGTTCTCTGGGAACAAGCTTTATGATTCTCATTAGAG TTTATTTGTTGATTGTCAGTAGTTGCGACTTTTAAATTATATTTCCCCCACTCAAAGAATGGTATCTTTA TATATCAATGACATTCAATAAATGTGTATTATTTCTAATGAGAA

Throughout this document, various references are mentioned. All such references are incorporated herein by reference, including the references set forth in the following list:

REFERENCES

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1. A method for characterizing multiple sclerosis in a subject, comprising: (a) providing a biological sample from the subject; (b) determining expression levels of at least two genes in the biological sample; (c) calculating one or more ratios of the expression levels of the at least two genes; and (d) comparing each ratios to a reference, wherein the multiple sclerosis is characterized based on a difference in the ratios of the expression values of the at least two genes in the biological sample from the subject as compared to the references.
 2. The method of claim 1, wherein the determining is of the expression levels of at least two genes represented by SEQ ID NOs: 1-47.
 3. (canceled)
 4. The method of claim 1, wherein the determining is of the expression levels of at least two genes corresponding to those set forth in Table A.
 5. (canceled)
 6. The method of claim 1, wherein the determining is of the expression levels of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP.
 7. The method of claim 1, wherein the determining is of the expression levels of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP.
 8. The method of claim 1, wherein the determining is of the expression levels of at least 2 genes corresponding to those set forth in Table B.
 9. The method claim 1, wherein the one or more ratios are ratios of expression levels of genes corresponding to those set forth in Table A, wherein each ratio is calculated by dividing the expression level of a first gene in Table A by the expression level of a second gene in Table A.
 10. The method of claim 1, wherein the one or more ratios are ratios are selected from those set forth in Table B. 11-15. (canceled)
 16. The method of claim 1, wherein the reference is a reference ratio of a comparator group, a standard reference ratio, or a healthy control.
 17. (canceled)
 18. The method of claim 1, and further comprising comparing each ratio to a second reference.
 19. (canceled)
 20. The method of claim 18, wherein the second reference is not a healthy control or wherein the second reference comprises other neurologic disorders (OND). 21-22. (canceled)
 23. The method of claim 1, wherein the characterization comprises diagnosing or prognosticating MS.
 24. The method of claim 1, wherein MS is predicted.
 25. (canceled)
 26. The method of claim 1, wherein the characterization comprises an exclusion of a diagnosis of MS.
 27. The method of claim 1, and further comprising providing a series of biological sample obtained from the subject; and determining a presence of any change in the ratios in each of the biological samples from the series. 28-32. (canceled)
 33. The method of claim 1, wherein the determining comprises a technique selected from the group consisting of a reverse transcription-polymerase chain reaction (RT-PCR), hybridization to nucleotide probes, and a Northern blot. 34-39. (canceled)
 40. A kit, comprising primer pairs for determining expression levels of at least two genes in a biological sample, wherein the at least two genes are represented by SEQ ID NOs: 1-47 or are set forth in Table A. 41-44. (canceled)
 45. The kit of claim 40, comprising primer pairs for determining expression levels of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP; or comprising primer pairs for determining expression levels of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. 46-47. (canceled)
 48. A device, comprising probes for detecting at least two genes in a biological sample, wherein the at least two genes are represented by SEQ ID NOs: 1-47 or are set forth in Table A. 49-52. (canceled)
 53. The device of claim 48, comprising probes for detecting each of the genes corresponding to CD55, FOS, JUN, PMAIP1, SPIB, TAF11, and TBP; or comprising probes for detecting each of the genes corresponding to ACTB, CDKN1B, CTSS, GAPDH-1, KRAS, PGK1, and TBP. 54-55. (canceled) 